FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAATGCTCAACAATATATTTAACACAACACGAGTAGAGAAGCCTAAA15.263157894736842No Hit
TGCCTAGTCTGATTAGCAACCTCAATGGCATCCGCTGCCTGTTCACTCGA15.263157894736842No Hit
AAAGGAATCACATAGACTACTACTGTACATTTCTTGTATTATAGTGAAAA15.263157894736842No Hit
GATCAGTAGGATCTGCTGAACAGGTGAAGCGCCAGCTGTGCCGTGTCATT15.263157894736842No Hit
GGGCACTAGGGGAAAATATGGCACCAGAAAAAGTAGACTTTGATGAATGC15.263157894736842No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA15.263157894736842No Hit
ATCTGGGCCGTTGCAGGACCAAGGTCATTGATTATCATGTAGTTCTTTAT15.263157894736842No Hit
CTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGACA15.263157894736842No Hit
ATCTAGAGGATCAGGCTGTCCGTGAAGCAACCCCCGGAGGATGATATGAG15.263157894736842No Hit
GAACCACAAAAGAGCCTGAATAGCCAAACAACCTTAAAAAAGGAAAGCAA15.263157894736842No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGTTTTAAACATGGTCCCTTCG15.263157894736842No Hit
CAGCAAGGTGGCGGACATGGTAGAAAGAGAAAAGGTGAGTAAATAAACCT15.263157894736842No Hit
GAATAAGAAGTGCTAACTGGCAAACTAAAAACCCTGAAAATAAGAGGAAA15.263157894736842No Hit
CATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATG15.263157894736842No Hit
TTAACATAATCAATCACTTTATCACTGACAAAGTGATTCACGCCATTACA15.263157894736842No Hit
ACGGGAAACTATGCAAAATAATAGGGGTCGCCCCATTGCATTGGGGAAAA15.263157894736842No Hit
AATTAATTGGGTATATTAATATAATTAAGGAAGAAAGCTATAATCCCGTA15.263157894736842No Hit
TTATGATGAAATAATATGCATTAACACATTCATTCGCGGGAAAGAGCCAA15.263157894736842No Hit
ATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGCA15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)