FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT12.857142857142857No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT12.857142857142857No Hit
AGCAGGTTCTGATCCAAAATGGAAGACTATGTGAGACAATGCTTCAATCC12.857142857142857No Hit
CCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGC12.857142857142857No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA12.857142857142857No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT12.857142857142857No Hit
CTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGA12.857142857142857No Hit
CAACCACCTCCTGAATATTCCGCCTGCTGATGAACAGTAAACACTCAATC12.857142857142857No Hit
GAATAGAACGATTCCTCTTTGGAATCCAGGAATGTGTAGTTGCAACGGCA12.857142857142857No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA12.857142857142857No Hit
CACTCACTGCCCCGAGACTGGAGCCACACAAATGGGAAAAATACTGTGTT12.857142857142857No Hit
ATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGC12.857142857142857No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA12.857142857142857No Hit
AAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGGGAAGA12.857142857142857No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC12.857142857142857No Hit
GCAGATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAA12.857142857142857No Hit
CAAATGGAGACACGAGGGGAGCGCCATGAAGACACAGAATGCAGACCAGC12.857142857142857No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT12.857142857142857No Hit
TAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGCATGT12.857142857142857No Hit
CCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGA12.857142857142857No Hit
GTGCAACACTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAA12.857142857142857No Hit
GTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGTTTCTCCCAGTAG12.857142857142857No Hit
GTTCTTTGCGTGACCAACGTGATGGTCAGGGTGTCGCTTTCTCTCCTTTT12.857142857142857No Hit
AGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTTTTTTGCC12.857142857142857No Hit
GTTGTGAGAAAGATGATGACTAATTCACAAGACACAGAGATTTCTTTCAC12.857142857142857No Hit
TGACACATTCTCTGAGTCGAAGTCTCTCAGTTATCCATCGTGCTCGGTTC12.857142857142857No Hit
GAACAACATGGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAA12.857142857142857No Hit
CCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTC12.857142857142857No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA12.857142857142857No Hit
ATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTTGACAA12.857142857142857No Hit
ATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGA12.857142857142857No Hit
CTGCAAAGATGTTGGAGACCTTAAACAGTATGACAGTGATGAGCCAGAGC12.857142857142857No Hit
GGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCA12.857142857142857No Hit
TCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCT12.857142857142857No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)