FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATA12.380952380952381No Hit
TTATGAGACTTCTAAAAGGATGACCAGAACATCGCAGAACGCCGTGTAAC12.380952380952381No Hit
CACTATTAGAGCAAAATCGTCTTATGATTGGAGACCATACAACAAGTATA12.380952380952381No Hit
TCCTTAGACAGAATCCCACTGAGGAACAAGCCGTAGACATATGCAAGGCA12.380952380952381No Hit
GGATTCTCACAGTAGGATTGCATGATCAAGTCAGTTAGGTTCTATCTGAA12.380952380952381No Hit
GGGCGTGGCTTGTCTCCGAAAATCACACTGCATATGTATCATATCAGATA12.380952380952381No Hit
CCATAACGGTTGCTCTTTCAAAAGGGAGCTTCCGCTGCACTGAGAATGTA12.380952380952381No Hit
ACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCC12.380952380952381No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA12.380952380952381No Hit
TTGATGCTGTGCCATCTCATAGAAGCTCCATTAGGTTCAGTGTTTACTTA12.380952380952381No Hit
GAATAGGATTGGCCCAACAACAAGTACAGTTCATTCCCCTAAGGTAGAGA12.380952380952381No Hit
GTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGCC12.380952380952381No Hit
ACACTGCTGCTCTTCTTACTATGTTTCTAGCAGCGATAATCAAAATTTGG12.380952380952381No Hit
CCCTGCTTCTTTAATTCATATGATATTAGCGGATGATGTTCTGTGGATGC12.380952380952381No Hit
CCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGT12.380952380952381No Hit
GACCGGTGGCACATTTCCAAGCGCGAAGCTAATCGGTCTGCTGACGCTCC12.380952380952381No Hit
AAAAAAGGGTACATAAACTAACTCAAGGTCGCAAGAATTATGATTGAACA12.380952380952381No Hit
CACTTGGCATAGCGCAGGAATCAAGACCATGTCTCCTATTTCAAGAAATC12.380952380952381No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA12.380952380952381No Hit
GAGTAGATGAATACTACAGCCAGGAGAGAGTGGTAGTGAGTATTGACCGA12.380952380952381No Hit
GGATGGGAACAGTGACCACAGAGGCTGCTTTGGGTCTAGTGTGTGCCACT12.380952380952381No Hit
ATATTACTGTTGGCTCTGTCTAGTGCTGATGGATTAGGTAGTTTTTATTT12.380952380952381No Hit
TTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATA12.380952380952381No Hit
CCTGGTTGCCTTTCTGAGAATAGCTGGTGCGCTTCTCCCACCCATTGTGA12.380952380952381No Hit
TCATTAAAAGCAAGAGTAAACAACATTGAACTAAAAAACGAAAAAGCCAA12.380952380952381No Hit
ATGGGATTCCTTTCGGCAGTCCGAAAGAGGCGAAGAGACAATTGAAGAAA12.380952380952381No Hit
CTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCAT12.380952380952381No Hit
GGTTTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCC12.380952380952381No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA12.380952380952381No Hit
TTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTG12.380952380952381No Hit
CATGCAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCACCAGCTAT12.380952380952381No Hit
CTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATAGAA12.380952380952381No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA12.380952380952381No Hit
CCGCTAATGAGTCAGGAAGGCTAATAGATTTCTGAAAGGATGTAAGGGAA12.380952380952381No Hit
GATATGTTCTCTGGCTAATCAATGTAATAATGTTTAATGTCTCCAAAATA12.380952380952381No Hit
ATCAGGCACTCCTTCCGTAGAAGTCCCTCGTTTAAAACCGTAGTTAAAGC12.380952380952381No Hit
GGAATACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGG12.380952380952381No Hit
GGGCGGGGTGGGCGGGGGCGAGTTGGGCCGGGCCGGCCGGGGGGTGGGGG12.380952380952381No Hit
GAACTGAGATATCTAATGTAGCAGTCAAGCACTCGCGAGATACTCACTAA12.380952380952381No Hit
AGGTCTTACTCATATCATGAGTTGGCATTCCAACCTGAATTATGCCACAT12.380952380952381No Hit
CAATAGGTGCCCCCCTCACATTATGATTGTAACAATAGTTAAGCAAAAGG12.380952380952381No Hit
ATGTTCAATAGGGTCAACATCTTATACTATTTCATCAAGTTCTATCCAGC12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)