FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S4_R2_D08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_D08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTAAACAATGGAAGTTGTTCAACAAACAAGGGTAGATAAACTAACTC19.090909090909092No Hit
ATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTC19.090909090909092No Hit
GTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAA19.090909090909092No Hit
CTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAAT19.090909090909092No Hit
TTGCAGGAGGGTTTACAATCAACTCCTGTCCCAGATCTTGGCTTGCAAGA19.090909090909092No Hit
AACTAACTCAAGGTCGCCAGACTTATGATTGGACATTAAACAGAAATCAA19.090909090909092No Hit
GTAACATCAGCTTCCTCAGTTCAGATCCTCTAGGTGATCTTGGAGGTGAT19.090909090909092No Hit
TTTCCCTAGTCTTGCCATTTTGTTTGAGAACATTATAGGTGCCATGCTCA19.090909090909092No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA19.090909090909092No Hit
TTCTATTAGAATCCATTTATAAATGGACAGCTAAGGCATAGAAAGATTGA19.090909090909092No Hit
CTCCTGCACCTGCTCCTGCACCTGGTCCTCCTCCTGCACCTGCACCTGAT19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)