FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3992
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA120.30060120240480964No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG80.2004008016032064No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC70.17535070140280562No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT60.15030060120240482No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG60.15030060120240482No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG50.125250501002004No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA50.125250501002004No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC50.125250501002004No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC50.125250501002004No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG50.125250501002004No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT50.125250501002004No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA40.1002004008016032No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT40.1002004008016032No Hit
ATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCC40.1002004008016032No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT40.1002004008016032No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT40.1002004008016032No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT40.1002004008016032No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA40.1002004008016032No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG40.1002004008016032No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA40.1002004008016032No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT40.1002004008016032No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG40.1002004008016032No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40.1002004008016032No Hit
TCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTT40.1002004008016032No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)