FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_D06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_D06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3785
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT80.21136063408190223No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA70.18494055482166447No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA60.15852047556142668No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG60.15852047556142668No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT60.15852047556142668No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC50.13210039630118892No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG50.13210039630118892No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGT50.13210039630118892No Hit
CCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAG50.13210039630118892No Hit
GTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA50.13210039630118892No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG50.13210039630118892No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA50.13210039630118892No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA50.13210039630118892No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT40.10568031704095111No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG40.10568031704095111No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT40.10568031704095111No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG40.10568031704095111No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT40.10568031704095111No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC40.10568031704095111No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT40.10568031704095111No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC40.10568031704095111No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT40.10568031704095111No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT40.10568031704095111No Hit
ATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTA40.10568031704095111No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGA40.10568031704095111No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG40.10568031704095111No Hit
CCAGGAGATTGATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATT40.10568031704095111No Hit
ATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTTT40.10568031704095111No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA40.10568031704095111No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG40.10568031704095111No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC40.10568031704095111No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG40.10568031704095111No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT40.10568031704095111No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)