FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_D03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_D03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCAAGCTTTCAGGTAGAATGTTTCCTTTGGCATATCCGCAAGCGATT17.6923076923076925No Hit
AAACAGTCCCTAACATTGGACTAAGCAATGCTTTCTAGGACATGACACCA17.6923076923076925No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTACAATCTGTGTGCTGTGTCAC17.6923076923076925No Hit
GACAGCAGAAAAGACATGTAGATGGATGCGGTGCAGAGTATAGTAAGGAA17.6923076923076925No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA17.6923076923076925No Hit
GTCCTACATTTGCTTATCATTGGGATCAGCTGAAAGTCATCCATGGCTGC17.6923076923076925No Hit
CGAATCGACTGACCCGGGTTGTGTATGCCTTACGCACTAGCGCCTTAAAA17.6923076923076925No Hit
GATTTTATCTCCTAGGTAACAGGCAAGAAAAATTTTGACAACATATCTGA17.6923076923076925No Hit
GTATTCCGCTCATTCCCATCACATAGTCGATGGATTAAATTCACCAGTTC17.6923076923076925No Hit
ATCTTGGGACTGTGAGTTCAAACCCCACATCAGAGATATAGAGATTACCT17.6923076923076925No Hit
TGGTAAGGGGATTTGCTCACAGATCGTACATGAAATGGAAATTAATAAGG17.6923076923076925No Hit
ATGTGGGAGATCAATGGCCCTGAGTCAGTGCTAGTCAACACTTATCAATG17.6923076923076925No Hit
ATCCCACAATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTT17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)