FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG50.3450655624568668No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG40.27605244996549344No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC40.27605244996549344No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAA40.27605244996549344No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG30.2070393374741201No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT30.2070393374741201No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG30.2070393374741201No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.2070393374741201No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.2070393374741201No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT30.2070393374741201No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30.2070393374741201No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.2070393374741201No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT30.2070393374741201No Hit
GTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA30.2070393374741201No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC30.2070393374741201No Hit
CTTTCTTTCCTCTTATGAAACTTGATACTCTTAAATCTTCAAATGCAGCA30.2070393374741201No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG30.2070393374741201No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC30.2070393374741201No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG20.13802622498274672No Hit
GCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGATTTATGCCT20.13802622498274672No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA20.13802622498274672No Hit
CTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTG20.13802622498274672No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAG20.13802622498274672No Hit
CCACCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCAT20.13802622498274672No Hit
CTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCA20.13802622498274672No Hit
GTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATACATTT20.13802622498274672No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG20.13802622498274672No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT20.13802622498274672No Hit
TCATCATCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTT20.13802622498274672No Hit
CTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAA20.13802622498274672No Hit
GACAATAACTTATTCGTCATCAATGATGTGGGAGATCAATGGCCCTGAGT20.13802622498274672No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC20.13802622498274672No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13802622498274672No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC20.13802622498274672No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT20.13802622498274672No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA20.13802622498274672No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT20.13802622498274672No Hit
GCACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACAT20.13802622498274672No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.13802622498274672No Hit
GTCTTCATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTG20.13802622498274672No Hit
CATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGAT20.13802622498274672No Hit
GGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAG20.13802622498274672No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT20.13802622498274672No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC20.13802622498274672No Hit
TCAGTCAAGAAAGAAGAAGAAGTGCTAACGGGCAACCTCCAAACACTGAA20.13802622498274672No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC20.13802622498274672No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA20.13802622498274672No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)