FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAG12.0No Hit
GAAATACACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACG12.0No Hit
AAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGC12.0No Hit
AAACAAGGCATTTTTTCATGAAGTACAAGATAAATTCATTATTTTTGCCG12.0No Hit
GGTCTGTAGGCTCCGATGGCTCATGGGGCGGAGGAAGGATGGGTGTGGGC12.0No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGACGAAAGAGGAA12.0No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT12.0No Hit
CTATCAGAATGCAGATGCATATGTTTTTGTGGGGTCATCAAGATACAGCA12.0No Hit
ATGATATTTGCTGCAATGACGAGAGGATCACTTGAATCGCTGCATCTGCA12.0No Hit
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC12.0No Hit
CCTGGAATCCTCCTCCTGTGAGGCCTCGTGTGGTTTGGGGCTGGCGCCTC12.0No Hit
ATCCTCCTGTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACT12.0No Hit
CATGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAAT12.0No Hit
TGAACAGTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGG12.0No Hit
GAATAGGATTGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATA12.0No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT12.0No Hit
TCTTGACTCAAGGGGCCTTGCTAAATGACAAACATTCCAATGGAACCATT12.0No Hit
ATGGCGCATGTCTCCTAACATCCATCTTGTAGGAGCAGGATGATTTTCTC12.0No Hit
GGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATCCATCTACTAGTGC12.0No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG12.0No Hit
GCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAGGC12.0No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.0No Hit
AAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGAG12.0No Hit
GGCAAATGTTGTGAGAAAGATGATGACTAATTCACAAGACACAGAGATTT12.0No Hit
TTCTCGATCCCTCACTTTGGGTCTTATTGCTATTTCCGGCTTGAACTTCT12.0No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT12.0No Hit
AGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAAC12.0No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA12.0No Hit
GTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAA12.0No Hit
CACATTCACAGTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGGGG12.0No Hit
AGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTG12.0No Hit
AAAGAAGGTCTATAACACTTAGGTCGCCACGGGCTCCAGGTGTAAACAGT12.0No Hit
GGATCAAGTAAGAGAAAGTCGAACCCCAGGAAACGCTGAGATTGAAGACC12.0No Hit
GGGATGCAGATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAG12.0No Hit
CCCTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAG12.0No Hit
CTATGAAGTGAATCCAAAATGCAACTTGATCATTACTATTCAAAAGATAA12.0No Hit
GAACCAATGCTTTTAGTTCTCCCTATCCAAACACCATTGCCGTATTTGAA12.0No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA12.0No Hit
TCTCCCAACCATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCA12.0No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA12.0No Hit
GACATGAGCAGACTGCCGAGGCCCAACGGCCGTATCAAAACAAGAGGGTA12.0No Hit
GAACAAAGAGGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAG12.0No Hit
GTCCAAAATGAATTCAATAAGGCATGTGAATTGACTGATTCAAGCTGGAT12.0No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA12.0No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT12.0No Hit
GTAGTAAACTTTAGTAGCCGTATTCAACTGTGGCTGAATGTAGAGTTACC12.0No Hit
AAATAAGCTGAAACGGGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGT12.0No Hit
CAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATT12.0No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT12.0No Hit
GCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTGGTCGGC12.0No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCC50.0145.01
TGTGAGA50.0145.08
TCCTCCT50.0145.02
CTCCTGT50.0145.04
CCTGTGA50.0145.06
GTGAGAA50.0145.09
CCTCCTG50.0145.03
CTGTGAG50.0145.07
ATACCCT50.0145.0145
TCCTGTG50.0145.05
Produced by FastQC (version 0.11.5)