FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_C12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_C12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAATAGAGCTCACAACCCACTTTCAAAGCAAAAGGAGAGTAAGAGACA12.7777777777777777No Hit
CAAGGAAGTTTTTTAGCAATAAAAAATCAGCTAACAGAAACCACGTATCG12.7777777777777777No Hit
GAATGAGTTAGGACCGTGCAAGGAAAATGATTTCAGAAATATACGATTTT12.7777777777777777No Hit
GTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTGGTGAATTAGT12.7777777777777777No Hit
GTGCCCACACTGCGAATTAATGATTTATGTAACTGCTTAGCACGTGTGCT12.7777777777777777No Hit
CTTTACCTACTTCGCGCTACCTTTATTCCATGCCCCTCTATGAAATGTCA12.7777777777777777No Hit
GTTGTATTCACTCCCCCTAGTGAATCGGGGATTCAAGACTTAATGTCAGA12.7777777777777777No Hit
GAATAATGGACATGAATCCAGAGAGGAATGAACAAGGACAAACCCTCTGG12.7777777777777777No Hit
CTCTGGGATAACAGTAGGGGGCAATGAAAAGAAGGCAAAACAGCCAAAAG12.7777777777777777No Hit
GTTATGGTGGGTTCACTTTCAAAATGAAAAGATGATCATCAGTCAAGAAA12.7777777777777777No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT12.7777777777777777No Hit
AACCACATGAGAAAGGCATAAATCCCAATTACCTCATGGCTTGGAAGCAG12.7777777777777777No Hit
ACCGGGAGCGGAAGCCAAACCTGCCTCCGCCGGGGACGCTGGCCCCTCCC12.7777777777777777No Hit
CCTTGGACCTGCAACCTCCCAGATGGCTCTTCAATTGTGTAGCATGTTGT12.7777777777777777No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGAACCCAACTGCATTTTT12.7777777777777777No Hit
ATTATAAAAAAGGGCAGTGGTGGTAAATGATTGATCTAAAATAGTCTAAT12.7777777777777777No Hit
GTATCCCACCGAACTTCACCATCAGTGAAAACTTTAGAGCATATGTATAT12.7777777777777777No Hit
GCTCAATCAATCAGGTGCAGCCAGGGATGACTTTCCGCGGCTCCCAATGA12.7777777777777777No Hit
CACAAGATCACACAATGCTATAAAACAAAATGGAAGTTGAACCATTTCAG12.7777777777777777No Hit
CTATATGATGAAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGACCC12.7777777777777777No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA12.7777777777777777No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC12.7777777777777777No Hit
CAAGAAGATTTAGATAAGGAACGGCTAGAAGGAAAAGCTGTACATGCGAA12.7777777777777777No Hit
GGATTATCCATTGATAAGTGTTGACTAGCACTGACTCATTGCAATTGATC12.7777777777777777No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTACAGTCTCTTGTCCCTAA12.7777777777777777No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG12.7777777777777777No Hit
ATTTCTCACTTCTCCTCCTGGAGTGTACCTCTGCTCCCAGCACGTCCCTT12.7777777777777777No Hit
CTGTGTTCTTGAAATAGGAGACATGCTCTTGAGGACTGCGATAGGCCAAG12.7777777777777777No Hit
GCTGCACTGAGAATGTAGGCTGCACACTGATCTGGCCTGCGGATGCCTTT12.7777777777777777No Hit
GGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGAATGATCAAGG12.7777777777777777No Hit
CCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAA12.7777777777777777No Hit
TTATCAGCCATTAGCTGGTTTCCTGTGTCCCAGCAAATGCTGAGGTAAGT12.7777777777777777No Hit
GAATGAAGTGGAGGCTGGCACTGCGCGACCCAAGGACCGCAGACAAGAGA12.7777777777777777No Hit
TAAAAAATAAAGCTAAGTTATAATTCTTCATTATTACCTCCTACAATAAA12.7777777777777777No Hit
ACTCAAGATGTGAGTCAGGCGCTTGGTCCGCAAGTGCTTGTCAGGATGGC12.7777777777777777No Hit
CCATCCAGCCATGGAACAGGAACAGGAGACACACTGGACACAGTAAACAG12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)