FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S4_R2_C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC42.7586206896551726No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT32.0689655172413794No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG32.0689655172413794No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT10.6896551724137931No Hit
TAATGAGGGGGGAGGAATAGTAAATAAGTGTTAAGGGAGGCAGTAAGCTC10.6896551724137931No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT10.6896551724137931No Hit
GCCTTCTTCAGTCTCTTCAGCAGATTGAGAGCATGATTGAGGCCAGTATT10.6896551724137931No Hit
CTGTGTAAGTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGA10.6896551724137931No Hit
GCCTTGTTGTAATTGAATACTGGAGAATTGCCTCTTACCAGTATCCTCAA10.6896551724137931No Hit
CACTTTTGGTTTCAATTTCTTTCCTGACATTACTTGCAATTATCTTAATT10.6896551724137931No Hit
ACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTATGG10.6896551724137931No Hit
GATCAAGACTTTGTCTATCACCAATGTTGTTCATTCAGTTGTCTTCTATA10.6896551724137931No Hit
CTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTC10.6896551724137931No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC10.6896551724137931No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGAAATCTT10.6896551724137931No Hit
GTTGGACTGGCCAGTGGCCAAAGCCTGGGCTAGCAAAGCTACTTTTCTCA10.6896551724137931No Hit
AATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGA10.6896551724137931No Hit
GCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTC10.6896551724137931No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA10.6896551724137931No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCGAATCGGTGCTTCAATAG10.6896551724137931No Hit
GGGGATGGGCTTGACCTGTCCGGGTCCCCTGGGGAGAGGCAGAAGCCAGG10.6896551724137931No Hit
AATGCTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCAT10.6896551724137931No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA10.6896551724137931No Hit
GTTTAATGTCCAATCATAAGTCTGGCGACCTTGAGTTAGTTTATCTACCC10.6896551724137931No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT10.6896551724137931No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA10.6896551724137931No Hit
GTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCA10.6896551724137931No Hit
TGCACACACTCATGAGCAAACCGCACATCAGAGGAATTAAAAGATGACCT10.6896551724137931No Hit
GTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATTACATATA10.6896551724137931No Hit
AAGCAGGTCTGCTTTCAAAAACCAGAAAAATACTACAGAAACATAACAAA10.6896551724137931No Hit
CCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGACT10.6896551724137931No Hit
CAGCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAA10.6896551724137931No Hit
TACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACT10.6896551724137931No Hit
GGTCTTCAATACTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGC10.6896551724137931No Hit
AAACAAACCTGTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAAT10.6896551724137931No Hit
ACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATC10.6896551724137931No Hit
GACATTTGAATTCACAAGCTTTTTTTATCGCTATGGATTTGTGGCTAATT10.6896551724137931No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT10.6896551724137931No Hit
TCATTGTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTT10.6896551724137931No Hit
CAATTACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTG10.6896551724137931No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT10.6896551724137931No Hit
GATTCATTTACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATT10.6896551724137931No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA10.6896551724137931No Hit
GCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGAAGTACTTCAGG10.6896551724137931No Hit
CTCTGAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGCGAATACCA10.6896551724137931No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10.6896551724137931No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10.6896551724137931No Hit
AATCAATATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCG10.6896551724137931No Hit
GCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCA10.6896551724137931No Hit
ATGGTGGACATCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACAT10.6896551724137931No Hit
AAAGAGAGTAAAGGACAAATTTACAGACACTGACAAGTATAAGGAAGCGA10.6896551724137931No Hit
TCTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10.6896551724137931No Hit
GAGACCGAATCATGGCCTGGACAGTGGTGAACAGTATATGTAACACAACA10.6896551724137931No Hit
ATCATGCAGTCTTAACTAAAATAAATTATTAGTTTAATGGCTTTTTGATA10.6896551724137931No Hit
GCCAATAAGATCATCAAACTTAACAGTGGTGCCCAGCAGGCCTTTAGCCC10.6896551724137931No Hit
CCCTAACTGAAGAAGTGAGGTCTGCCCAAAATGTACTATCCTTGCTAAAC10.6896551724137931No Hit
GGGGGAGTGATCAGCTCCTCTACCCTGTTCCTGCCTGTGGATTCTGTTCC10.6896551724137931No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA10.6896551724137931No Hit
GGAGATAGGGCGAAGGGAGGGAGAGACTCTGAGGCAGAGGACTCTGTTGT10.6896551724137931No Hit
AGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGA10.6896551724137931No Hit
CAGCAGGGGAAGTGCTTATGGTTCCTCCCCATCCCACACCATTATGCAGC10.6896551724137931No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTC10.6896551724137931No Hit
GACTTTGGTCGGCAAGCTTGCGCATAGTTCCTGTAATCTCAAATTTTTCT10.6896551724137931No Hit
CATGAAAATCTTCTATTTTTAACTTCAACACTCTCTTCCTTAATCCAAAT10.6896551724137931No Hit
GGCCACCAAAGCGTACTACACCCTTGACGAAGAGAGCACGGCAAGAATCA10.6896551724137931No Hit
GTCATGCTGTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTC10.6896551724137931No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT10.6896551724137931No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT10.6896551724137931No Hit
GTCCTCCTCCTGCACCTGCTCCTGATCCAGATCCAGCACCTGCTCCTGCA10.6896551724137931No Hit
ATATAGGACTTCTGTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT10.6896551724137931No Hit
GAAACAAGGCAGTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCC10.6896551724137931No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA10.6896551724137931No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC10.6896551724137931No Hit
TTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGAT10.6896551724137931No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG10.6896551724137931No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC10.6896551724137931No Hit
TGGCGAAGAGGCCCATCAGGCAATCTGAGGGGGCGTGGTGTCGTCCTCAA10.6896551724137931No Hit
ATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCG10.6896551724137931No Hit
ACCATATGCAAAGTACGCTCCATGGGTCAGTTATGTCCCATCGGTCTTTC10.6896551724137931No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA10.6896551724137931No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAC10.6896551724137931No Hit
GGCCATGTTTCCGATTTGTTTTCAAAGAATTCCTTGGTCATGTCTTTCTC10.6896551724137931No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT10.6896551724137931No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGA10.6896551724137931No Hit
GTGAGAGGGCCTGCGAGGTCCACAGTCAGAACTCGTGTAACGCCCAACTT10.6896551724137931No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG10.6896551724137931No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA10.6896551724137931No Hit
AAAAAAAGCTTGTGAATTCAAATGTCCCTGTCTTATTTATATAGGACTTC10.6896551724137931No Hit
TCTAAGCAGTAATCAAAGTCTACTATTTCTGATGCCAAATTTTCAACGAG10.6896551724137931No Hit
TCGACGAACAGGGTGAATCAATATTCAACAGTCTATATGCGTCTCCACAA10.6896551724137931No Hit
AGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAAC10.6896551724137931No Hit
GTTCCTGCTGATGGATGCTCTGAAATTAAGTATTGAAGACCCGAGTCACG10.6896551724137931No Hit
TGTCTGTCCATACAAACAAGGCTAAACATCCCGGAATCCAGCCACACGAG10.6896551724137931No Hit
GAGGGAGGGTCTGAGGGACATCTTGGGCCCACCACACCTCTCGGAATCCT10.6896551724137931No Hit
GTAACCAGAGAATTCACGGGCGAAGTTTTCAGAAACCAATCATTGTTCTG10.6896551724137931No Hit
TCAGAAATCAAGTTAAAATAAGGAGGAGAGTTGATACAAACCCTGGCCAT10.6896551724137931No Hit
ACTTGAGAGATAACAAAAACAAAGACACTGTGGAACTATAAAACCTGTCA10.6896551724137931No Hit
CTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGACCCGAGA10.6896551724137931No Hit
GGGGATCTCTGATATGTAGAAGTCTCCTTCTCCCTCAACCTCTCTCTCCC10.6896551724137931No Hit
CGTCCGAGCTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTC10.6896551724137931No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10.6896551724137931No Hit
GAACTCTTCTTTCATGATCCGTCAAGACACGAAGTCTACCCTGACATCAA10.6896551724137931No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT10.6896551724137931No Hit
TGGTCATGTTGTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATC10.6896551724137931No Hit
GACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAA10.6896551724137931No Hit
GGGTGAATCAATATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGT10.6896551724137931No Hit
GTGAACATCTGCTCCCAGCACGTCACTTGGGTTAACTGCAACACTTCAAT10.6896551724137931No Hit
TTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGT10.6896551724137931No Hit
CTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCC10.6896551724137931No Hit
GCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAG10.6896551724137931No Hit
ACATATCGCAAGCATGAGGAGGAACTATTTTACAGCAGAAGTGTCCCACT10.6896551724137931No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA10.6896551724137931No Hit
GTGTAGAGGATGGCTTGGAAAGGGAGGGACTGGACGTACAAAAAGTACAC10.6896551724137931No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCAT10.6896551724137931No Hit
ACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATT10.6896551724137931No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG10.6896551724137931No Hit
TTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCT10.6896551724137931No Hit
GTAGAGTAGAGCCAGAATTTATTCCCAGCACTCAAATCCCACACTATACT10.6896551724137931No Hit
CAAATATGCATAATATAAAATGGTAGTTCTATTTTAAATTTTTGAGGACC10.6896551724137931No Hit
CATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGAT10.6896551724137931No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT10.6896551724137931No Hit
CACTGAAGTTTCTCATGATCATTCGAGATCATGACTAAACCAAAGTTAAG10.6896551724137931No Hit
GATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAATCACTGGGG10.6896551724137931No Hit
GATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTT10.6896551724137931No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10.6896551724137931No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC10.6896551724137931No Hit
CAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGT10.6896551724137931No Hit
TACAGGAACTATGCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCC10.6896551724137931No Hit
GAATAAGAGAGGCTATCTAATAAGAGCGCTGACATTAAATACGATGACCA10.6896551724137931No Hit
ATGATGATGTTGACCAAAGTTTGATTATCGCTGCTAGAAACATAGTAAGA10.6896551724137931No Hit
TTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAAGCCAAAGGG10.6896551724137931No Hit
TCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAG10.6896551724137931No Hit
TCTTTATTCAGTAGCAGCTACTGAGTGGCTGACAGGAAGGAGATCCCTGG10.6896551724137931No Hit
GGCAAGACCGCAGGAAATTTCACAGCACATTTCAGACACTGTCAGTCCAG10.6896551724137931No Hit
GTTAAGTGCAACACTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGG10.6896551724137931No Hit
CATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGAGCATG10.6896551724137931No Hit
TCACAAGACACAGAGATTTCTTTCACAATCACTGGGGACAACACTAAGTG10.6896551724137931No Hit
GGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAGAA10.6896551724137931No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers