FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S4_R2_C08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_C08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAA21.3605442176870748No Hit
GAATTAATCTCTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCT21.3605442176870748No Hit
CCGCTGCCTGTTCACTCGATCCAGCCATCTGTTCCATAGCCTTTGCCGTA10.6802721088435374No Hit
CAGATAAGCTGTGCCTGTATTCCTGACCCACAGAAATGGTGATATAAGAA10.6802721088435374No Hit
AAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCG10.6802721088435374No Hit
CCACTAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCACTACGGCAAA10.6802721088435374No Hit
CAATTGTTCATCAAAGACTACAGATACACATATAGGTGCCATAGGGGAGA10.6802721088435374No Hit
GTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCT10.6802721088435374No Hit
ATGTGATGGGAATGATCGGAATACTGCCCGACATGACCCCAAGCACGGAG10.6802721088435374No Hit
TCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGC10.6802721088435374No Hit
TGTTCATTGATTCCATTACATCCTTTAAGAAATCTATTAGCCTTCCTGAC10.6802721088435374No Hit
CCTTCCTTTTATAATGAACCCATACAGGTTTGTTTTCCGTCTTCCTTCTT10.6802721088435374No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA10.6802721088435374No Hit
GGATGTAATGGGATCAATGAACAAAGAGGAAATAGAGATAACAACCCACT10.6802721088435374No Hit
GCTGAAAGTCATCCATGGCTGCACAGGATGCATTGAGCAAGGCCGTATTT10.6802721088435374No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG10.6802721088435374No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG10.6802721088435374No Hit
GCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAACA10.6802721088435374No Hit
AAATAGCCCAAAGTTCCGCTCACTAGGAACATGACACCCTTGATCAACAC10.6802721088435374No Hit
CTATTCACTCCCTCATCGACTGAATGGGGTATTCCCTGACTGCCGCAACC10.6802721088435374No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA10.6802721088435374No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10.6802721088435374No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG10.6802721088435374No Hit
GAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACA10.6802721088435374No Hit
CCGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCA10.6802721088435374No Hit
GGACATTTGAATTCGCAAGCTTTTTTTATCGCTATGGATTTGTGGCTAAG10.6802721088435374No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT10.6802721088435374No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGATG10.6802721088435374No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA10.6802721088435374No Hit
TTGTAATCCCCTGAATCCCTTTGTCAGTCATAAAGAGATTGATTCTGTAA10.6802721088435374No Hit
GAGCAGGAGAATTTGCTGGAACTTGAGCAGGTCCTGGAATAGGATATATA10.6802721088435374No Hit
CTTAACAACTGAGCCACCCGAGCACCCCCCCTTACCATTTTCTTGATAAC10.6802721088435374No Hit
AATAAAGTCCTATTCACATTTACGCGCCAATTCATGGCGCAGATAAGTAT10.6802721088435374No Hit
GTGTGTGTGTGGGGGGGGAGGGGGATGGAGGTAAAAAATGACCGCTTCCA10.6802721088435374No Hit
GACTCCATCCTGAGTGCAGAGCCCGACCTGGGGCTCAGACCCACAATTCT10.6802721088435374No Hit
GCCCAGGCATAAGTGCTGCGGTGCAATCGCTTAGAAGCTGCCTCAGTGGC10.6802721088435374No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC10.6802721088435374No Hit
GTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATG10.6802721088435374No Hit
TAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGACCCCCGCACTCAGA10.6802721088435374No Hit
GGTTCATTATACAAGGAAGGTCTCATTTGAGAAATGATACTGATGTGGTG10.6802721088435374No Hit
ATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGA10.6802721088435374No Hit
GTATTACATCAAATATGTAGATTTAGGGTAGTATAGACCTCTTAACAATA10.6802721088435374No Hit
TGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCAT10.6802721088435374No Hit
GTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGT10.6802721088435374No Hit
CCTATATGAACTGCTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGT10.6802721088435374No Hit
CCTCGACACTGTTGGGGCCTCTTTGGCCATACTTTCACTCCCCCACCACA10.6802721088435374No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10.6802721088435374No Hit
GTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTG10.6802721088435374No Hit
GATTCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCT10.6802721088435374No Hit
CTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATTTAATGTCAG10.6802721088435374No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC10.6802721088435374No Hit
GCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATTCAGT10.6802721088435374No Hit
ATTTACCCCTCACCGAACCGGGCAGGCGGGTGCCTGTGTCCCTGCTCCAT10.6802721088435374No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG10.6802721088435374No Hit
AAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGGAATCAAC10.6802721088435374No Hit
CTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCAATGCAGTT10.6802721088435374No Hit
GGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA10.6802721088435374No Hit
GATAAGTGTTGACTAGCACTGACTCAGGGCCATTGATCTCACAAATCATT10.6802721088435374No Hit
ATCTAAAGACTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTT10.6802721088435374No Hit
TTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTG10.6802721088435374No Hit
CACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGAAGCTGTGGGATC10.6802721088435374No Hit
CATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGG10.6802721088435374No Hit
GGCATTACCACAGCATTGTTTACAGAATCAATCTCTTCATGACTGACAAA10.6802721088435374No Hit
GCCCCCTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAG10.6802721088435374No Hit
GTCATGGATGCCAGTTACAGTGTACTATTTCAAAACTTTGTATAGCCTTC10.6802721088435374No Hit
GTTGACTACCACTGACTCAGGCCCAATGATCTCCCACATCAAAGAAGACG10.6802721088435374No Hit
TCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGGTC10.6802721088435374No Hit
CTATAGACATCTTAAAAATATTGTTATTCCAATAAATGAGCATGGATTGT10.6802721088435374No Hit
CAGAACAGTTCAGGCGGCTGGCTCAATCCCTACTCCATATGGACTCTGAA10.6802721088435374No Hit
CCTTGAGTTAGTTTATCTACCCTTGTTTGTTGAACAACTTCCATTGTTTC10.6802721088435374No Hit
GCTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCAATGCAGT10.6802721088435374No Hit
GCCTAACAGCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTA10.6802721088435374No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG10.6802721088435374No Hit
GGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATC10.6802721088435374No Hit
ATGCTCAACCATCTGAGCCCCCCAGGCGCCCCGAAATTTAATGAAAATTT10.6802721088435374No Hit
CTATGACACAGCTTTGTGTCGTGGTCCTTCAGCCATCAAGGCTCAAAGTC10.6802721088435374No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC10.6802721088435374No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG10.6802721088435374No Hit
TCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGCATTCACTATGA10.6802721088435374No Hit
CATTAGCTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCAAT10.6802721088435374No Hit
ATATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT10.6802721088435374No Hit
TTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCA10.6802721088435374No Hit
AATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAAGTAGACTTT10.6802721088435374No Hit
CTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGG10.6802721088435374No Hit
CTCCTAGCTAAAGTTTCAACAAAGTATACGAAACCTCTAATCTGCATCCC10.6802721088435374No Hit
CAACCCGAGTGGTTCAGAAACATCCTGAGCATGGCACCTATAATGTTCTC10.6802721088435374No Hit
CCATACAACCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG10.6802721088435374No Hit
AGAAAGAGAATTGGTCCGTAAAACAAGGTTTCTCCCAGTAGCCGGCGGAA10.6802721088435374No Hit
ACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGCT10.6802721088435374No Hit
TAACAGAACAACTTTCAAAATGTCCCAATTCATCGGGTCCCTCGCAGCCT10.6802721088435374No Hit
CTATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC10.6802721088435374No Hit
CTTTAGGACTGACAAAGGGATTCAGGGGATTACAAATTCTGCCCCGATAC10.6802721088435374No Hit
GTATTCCCTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCGTCGGAT10.6802721088435374No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG10.6802721088435374No Hit
GTAATCCCCTGAATCCCTTTGTCAGTCATAAAGAGATTGATTCTGTAAAC10.6802721088435374No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC10.6802721088435374No Hit
GCGCACATGTGTGCACACATGAATATGTGTGCGCGCGCATGTATGTACAT10.6802721088435374No Hit
GAAACATCCTGAGCATGGCACCTATAATGTTCTCAAACAAAATGGCAAGA10.6802721088435374No Hit
ATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACA10.6802721088435374No Hit
GAACCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGTCCTACATTTGC10.6802721088435374No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTC10.6802721088435374No Hit
GTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAA10.6802721088435374No Hit
GGCTAGACCATGGGCTGGCATTACCACAGCATTGTTTACAGAATCAATCT10.6802721088435374No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT10.6802721088435374No Hit
CACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAG10.6802721088435374No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGAAGTAACAGTGATAA10.6802721088435374No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC10.6802721088435374No Hit
GATTTGTATGATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACG10.6802721088435374No Hit
ATTTGGAACAGGACACTCACCAGGACTTAGCTGTGCTGCTGTGATCTGGG10.6802721088435374No Hit
GGATTAAAGAGATAAAGCTGTCCTTTGATAAAAGATGAAAAAATAATTAC10.6802721088435374No Hit
GCCAGGAGCACCCTCCGGCCTCCTTCCTCATCCGCAGTCTTCTCTTGAGT10.6802721088435374No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC10.6802721088435374No Hit
AAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTA10.6802721088435374No Hit
TTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTT10.6802721088435374No Hit
TCGACAATGTGATGGGAATGATCGGAATACTGCCCGACATGACCCCAAGC10.6802721088435374No Hit
TGCCATTTTGTTTGAGAACATTATAGGTGCCATGCTCAGGATGTTTCTGA10.6802721088435374No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA10.6802721088435374No Hit
GGGAAGTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTA10.6802721088435374No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT10.6802721088435374No Hit
TTCCATTTCCTGTCCGGGACTTCCTATCCACTTAATCTGACACACAGACC10.6802721088435374No Hit
CCATGGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA10.6802721088435374No Hit
GAAACAAGGAGGGGAACCAAAGGTCGAGGTGGCAGAGGGGAAATGAATGG10.6802721088435374No Hit
CTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAAT10.6802721088435374No Hit
GTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGACCCGAGACTGGA10.6802721088435374No Hit
GTGTAGGGTGACCTGACTCCATCCCCACTCCAGAACTACGCCTCAGCACC10.6802721088435374No Hit
AGCATGGATAGTCTTCCCGTTTGTTTTTGCCATCTTCAATTTCTTTCATC10.6802721088435374No Hit
CCCATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTATATTGTATAAG10.6802721088435374No Hit
CATTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGT10.6802721088435374No Hit
AGGCTGCACCATCATCTCTGCCCCACTCACCTCCTCCTCAATGCCTGCCT10.6802721088435374No Hit
GGACTCGCCTCAGTGAGCGTCTTCGTGGAGCCCGGGCTGAGACTAAAGGC10.6802721088435374No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA10.6802721088435374No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA10.6802721088435374No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG10.6802721088435374No Hit
ATACTGGGGTTTCTTTCAAGGCTCAGACAGCTTTTGACTTTTCTAGAAAA10.6802721088435374No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC10.6802721088435374No Hit
TTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGAC10.6802721088435374No Hit
TCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGT10.6802721088435374No Hit
GAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGA10.6802721088435374No Hit
CCCGAGAAGAGCACAGGCTCCTGCTCCACCGAAGTCACCTGTCGATGCAG10.6802721088435374No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA10.6802721088435374No Hit
TGGCTGGAAAGAGCCTAACATAGTCAAACCACATGAGAAAGGCATAAATC10.6802721088435374No Hit
GGTTCAAATTCCATTTTGTTGTATAACATTGTGGGATCTTGTGACCATTG10.6802721088435374No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA10.6802721088435374No Hit
CCCTTCATTATGTCTTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAA10.6802721088435374No Hit
CTTTCTGAGGTAATTTATTTGGGGTTCGATGTCAGTTGTCACATGTGATA10.6802721088435374No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers