FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_C01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_C01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCA12.5No Hit
GAATGGGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCC12.5No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT12.5No Hit
CCATGGCCTACACCATGCTAGAAAATCCAACCCCTCTCGAATATCAACTG12.5No Hit
ATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGC12.5No Hit
CCACCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCAT12.5No Hit
GAAGAATCCCACCCAGGAATATTTGAGAATTCATGCCTTGAAACAATGGA12.5No Hit
ATTCAGTAGCCCTGCAGTAGGACACTTCAGCTGTATAAGAGTTACTCATC12.5No Hit
GCTTTGCACTTTCCATCATTCTTATAACTTCTGTTCGCATGTCGGATGTC12.5No Hit
CTGGTGATATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTC12.5No Hit
ATGCAGCTCTCAGTGCCAAGGAGGCACAGGATGGGATTATGGAAGTTGTT12.5No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG12.5No Hit
CCATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTT12.5No Hit
ACTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATGG12.5No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA12.5No Hit
TACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTAGAAT12.5No Hit
AGGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTG12.5No Hit
CTTCATTATGTATTCGGTAGCCCGGCTGTGGGACGCTTCTGCTGTAAAAT12.5No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC12.5No Hit
CAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATAC12.5No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG12.5No Hit
CTAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCACTACGGCAAAGGC12.5No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG12.5No Hit
GTCATTAATTAAGCTTGCGGCGCGGGTTTGTTAGACGACCTAATCTAAGT12.5No Hit
GTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAG12.5No Hit
AGAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTGATGGTGGCGTAC12.5No Hit
GTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATAT12.5No Hit
CCAGGAGATTGATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATT12.5No Hit
CACAAATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTCCCTGTCT12.5No Hit
TCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTG12.5No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCCTC12.5No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT12.5No Hit
GAGTTGAATGTGGTGCGGTCCAGGTTTCAGGAGCTGATGGAGTGGGCAGA12.5No Hit
GTAACACGGAGGGAAGTCCACATATATTACCTAGAGAAAGCCAACAAAAT12.5No Hit
GAAAGTTAAGCTACGACCAATACCGAAAACCAAGTTGACAGTCGCCACGC12.5No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA12.5No Hit
GGTTCAAATTCCATTTTGTTGTATAACATTGTGGGATCTTGTGACCATTG12.5No Hit
TGAAAACAGAATGGTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGA12.5No Hit
CAATAGCAATGGAGTTAATCAGAATGATCAAACGTGGAATCAATGACCGA12.5No Hit
CATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGT12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers