FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_B12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_B12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGAGAGCTTATGGAACTAAGAGCACTCTACTTTCTTGATAAGTGCAC15.555555555555555No Hit
GCCTAATGCGATCAACCTGGCCCTGTGTCGAATCACCCTGATTTTTATTA15.555555555555555No Hit
CTTACACATACAGTAAAGTCCCTTCATCCTCAGAACACGACTAGACTAGG15.555555555555555No Hit
GAGGAGGAGACTAATATCTCACTAACATGATTTCACAAAGGTGAGCAAGA15.555555555555555No Hit
CCATTAAAAGGCATCTAATCGAACTTGTAAATCGGGGAACAGAAAGTAAA15.555555555555555No Hit
GCCTTATGATGGCTAACAGCCATCTGGTCAAGTCTGCCCAACACTAGACA15.555555555555555No Hit
ATAACTAGCAAACTATAGGTTCAAGAGTTTATAACCCAACATTCAGCCTA15.555555555555555No Hit
ACCCAGGCACCCTGGAAGAATGAGATTTTAATAGAATAGAATCTGAACAA15.555555555555555No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCACCATCATCATTTCTCACTT15.555555555555555No Hit
AAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATT15.555555555555555No Hit
AGTACAGACATGGAATTTTTTCATGAAGGAGAATTTAATTTATTTATTAT15.555555555555555No Hit
TAATTCGTCTGTAACACAATGCAGGTTTGCAACACCCTTAACTACGACCA15.555555555555555No Hit
TCCGCCCCATGTTCGAGCTCTCCAGTCCAGTGCTGCGCTCGGGGGCCCTC15.555555555555555No Hit
CTCATTAGCTCAGGCAATAATACTGGGCATGGTATTGTGACTGGAGGCTT15.555555555555555No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT15.555555555555555No Hit
GGTCTGAGCGGGCACATGTTGGGTGGCTGCCTCGCTGCGCTTGGCTGGGC15.555555555555555No Hit
GTTTGGTGCATTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCAT15.555555555555555No Hit
GGCTTGACATTTCCTGGTACAATGGGGAGATGCAGTAGGCAGTTGTTTGA15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)