FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_B07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_B07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4383
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA170.38786219484371437No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT150.34223134839151265No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG140.31941592516541184No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG110.25096965548710926No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC110.25096965548710926No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT100.22815423226100845No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG100.22815423226100845No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC90.20533880903490762No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG90.20533880903490762No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT90.20533880903490762No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT80.18252338580880675No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA80.18252338580880675No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA70.15970796258270592No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT70.15970796258270592No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT70.15970796258270592No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC70.15970796258270592No Hit
ACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCAAATGATCTTCT70.15970796258270592No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG70.15970796258270592No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG70.15970796258270592No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.15970796258270592No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG70.15970796258270592No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT60.13689253935660506No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT60.13689253935660506No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG60.13689253935660506No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC60.13689253935660506No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA60.13689253935660506No Hit
GTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACAC50.11407711613050422No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG50.11407711613050422No Hit
TCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGT50.11407711613050422No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG50.11407711613050422No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC50.11407711613050422No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT50.11407711613050422No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC50.11407711613050422No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA50.11407711613050422No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT50.11407711613050422No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT50.11407711613050422No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG50.11407711613050422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATTC100.006854927144.9773
AATTCAT100.006854927144.9775
GTTAAAT100.006854927144.9771
ATAAGAG450.00901692748.3256723
Produced by FastQC (version 0.11.5)