FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGAGGCAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGT12.5No Hit
GTTAATATCTCACAGAATGGCGGTTAGACGTCACGCATACTGTTAGACTC12.5No Hit
GGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTATCATCAAGGAC12.5No Hit
GTTCCTGGGATGTCGTTAAACCTTTCTCTCCATCCGTGTTTGGTGGAGAT12.5No Hit
TAGTAGCCATCTGTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCA12.5No Hit
GCTCTGGGCAAGCTCTGGGGGGAATCGTATAAAAGACCACACAAGAAAAA12.5No Hit
GACTGATGATCCGCTTGTCCTTTTGAAAGTGAACCCACCAAAACTGAAAG12.5No Hit
CGCCGAGCCCTCCAGTGTCGTGGGTGGTATCACACAGCGGAGTGCACTCA12.5No Hit
CTAATTTACAGCAGAAGTGTCACACTGCAGGGCTACTGAATACATAATGA12.5No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGAACATTTCCAAGAGAGATGCT12.5No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC12.5No Hit
ATGTAAAATTTCCCCAGCACATACCGCATTTGCTGGAACAGTGTCCTAAC12.5No Hit
CTGTCCTTACTAATCCACTCTACCGGCTTCTGGTTGCCTTACGGACAATA12.5No Hit
AATCTAGACTGTACAATTCAAGATTACAACCTTAACTCCTAAATAGTCAA12.5No Hit
GACCTACGAAGTGATTTTCCCGGGGTGAACATGAGAGGGCACCCCCCGGT12.5No Hit
ATATGAACAGCTTGGGAATAATTTATCTAATAGATTGCAGCACTTCTGGT12.5No Hit
GCTCAGTACACTTACATTGTGTAAATATATACACTACAAAAATTAAAAAC12.5No Hit
AATGCCAGCTGTGACTCTGAAGTCACTAATCTTGCCCCCACTACAATAGG12.5No Hit
TAATAAAAATTCTCCCCTTTGCTGCTGCCACACCAGAACAGAGTAGGATG12.5No Hit
CATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGAT12.5No Hit
CCATTATACTCCATATCATCCAAACACCCAACGAACAGGTACAGGATACA12.5No Hit
CTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCAATGCAGTT12.5No Hit
CTCTTATTCATTGTTTGTTATTTCTTAACAATAGTTCTTTGCGTGCCCAG12.5No Hit
GTAAGGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTTTCA12.5No Hit
GCACTAATGAGTCTTCTAATAATCAGCTCGTGGTTTTTTGTTTAACTTCA12.5No Hit
GGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACT12.5No Hit
GCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGATCCAGTTGATAGT12.5No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC12.5No Hit
AACCCAATCAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAACTTAT12.5No Hit
AATCATGACGATGCTAGTATATTGCACATCATCAATTATGAATGTGCTTA12.5No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTATAGTGGTTGGTGATACATTG12.5No Hit
CAAAGTCTCTATAAGAATGAAGATTCATATGTTTTTGTGTGGTCATCAAG12.5No Hit
GAACCAGTTTACCGCAGAAGTTGCCCACTGGCGGGCTACTGAATACATAA12.5No Hit
CCATGATTCTAATATCTTCTGTTCGCATGTCGGAAGTCCGTCCTTCATTG12.5No Hit
ATCTCAAATCGGTGCTTCAATAGTGCATTCGGGTCACCAGATTCTACAAT12.5No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG12.5No Hit
CACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTG12.5No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG12.5No Hit
CTTCATTCTTCAATGATGATCAGCATGTCCATTAGAAACTGAACACACAA12.5No Hit
ATTACAAAGAGAACCGAATAATGGAAAATGGACTAACACGGAGGGAAGAC12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers