FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_B04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_B04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA21.9801980198019802No Hit
CCAAAGGTCTATGTGATAATTTTTGTTACGATAAGTAGTAAGATGTTTGG10.9900990099009901No Hit
GGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATAGTCAAATCAG10.9900990099009901No Hit
CCATAGAAGTCGTTAGCTCGAATGGCGGAGCAGCGAATGAGGCGGGATGG10.9900990099009901No Hit
TTCGTAAGGACACTGTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGA10.9900990099009901No Hit
GTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACC10.9900990099009901No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAGGCAAAACCCAGGGATC10.9900990099009901No Hit
GCGCCGGGGGGGGGCGGCTTGGTTCTGGTGAGGGTGTGGGGGCGCGGGGC10.9900990099009901No Hit
CCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTCT10.9900990099009901No Hit
ACGGAAAACAAACCTGTATGGGTTCATTATAAAAGGAAGGTCTCATTTGA10.9900990099009901No Hit
CACCCAGGGGTGTGGAGGATGGCTCTTTTGGGAAAGTCTGCAGGTGGTTA10.9900990099009901No Hit
GCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAACC10.9900990099009901No Hit
GAAAGCAGGGGAGTGGGAGAGGGAGAAGCAGGCTCCCGCCGAGCAGGGAG10.9900990099009901No Hit
CTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTTC10.9900990099009901No Hit
CGTCTGGTACATCTGTTCATCCTCAAGAATTCCCCGTTGGCTTGTGTTGA10.9900990099009901No Hit
GGGCCTCGGTGGGCGGGCGAGGCGGGTTCGAGGTGCTCGGAAAGGGCCAG10.9900990099009901No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT10.9900990099009901No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA10.9900990099009901No Hit
GTGCCCAGCAGTAGGTAAAGAGGTCAACCCCCTGGAAAAAAGATTAGAGA10.9900990099009901No Hit
CATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAATATAAA10.9900990099009901No Hit
CATTTGGAAGTTTGGGTCATGTATTCGTATGTCAATTTCATCTACGAACG10.9900990099009901No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA10.9900990099009901No Hit
CCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTCACAGTCAATGA10.9900990099009901No Hit
GTTAAAGATTTAGGATACACTCACCAGACCCATTGGCAGCTTTCAGTACA10.9900990099009901No Hit
AGCTGGTTGACTTCGAAAATGCATGTCTAAGTGCCTCAATGCTACAAAGG10.9900990099009901No Hit
AGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAAATGAATTCAATAAG10.9900990099009901No Hit
TCTGAGAATAGCTGGTGCTCNTCTCCCAACCAGTGTGAATNCTTCAGACC10.9900990099009901No Hit
GTAAGGCTACCACAATTCATCAAAAAGACAACGCCTTTACTTCTTGAGAT10.9900990099009901No Hit
GAGCTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAG10.9900990099009901No Hit
ATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAG10.9900990099009901No Hit
ATCCAGCTTGAATCAGTCAATTCACATGCCTTATTGAATTCATTTTGGAC10.9900990099009901No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT10.9900990099009901No Hit
GTCATACTACTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA10.9900990099009901No Hit
ACAAAGTTCAGATCGCCCCTAACTGCCTTGATCATGCAATCCTCCTGTGA10.9900990099009901No Hit
GTTGTATAACATTGTGGGATCTTGGGGCCCTTGAATTTTCACAATTTCCC10.9900990099009901No Hit
ATCCTGAGCATGGCACCCTTAATGTTCTCAAACAAAATGGCAAGACTAGG10.9900990099009901No Hit
ATCTTCTCCTATTTCATCAAGTTCTATCCAGCTTGAATCAGTCAATTCAC10.9900990099009901No Hit
GCAATCTGGACCCCTCTTGTGGAAAGCTTTCCTCTTGGAATCCCTTTCTT10.9900990099009901No Hit
ATCATCATCCATTAGCTCCCATTTTAAGCAGACTTCAGGAATGTGAAGAT10.9900990099009901No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC10.9900990099009901No Hit
CTCGAAGACTCCCCGCCCCTGGAAGGACAAATCTTCTGGCTTTGCACTTT10.9900990099009901No Hit
CATGTACCCGTTCCCTAGTCTAGCCATTTTGGTTGAGAACATTATGGGTG10.9900990099009901No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA10.9900990099009901No Hit
ATGGTGGGGTCGGGGTTGGGTTGGGGTTGGTGGGGTGGGGGGCGGGGGGG10.9900990099009901No Hit
TTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCA10.9900990099009901No Hit
CGTCTGCGCCTGGAGTGTACGTCTGCTGGCAGCGCGGCCCGTGGGTTACG10.9900990099009901No Hit
CAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGCCAATGG10.9900990099009901No Hit
TTTATAATGAACCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGTCCT10.9900990099009901No Hit
GAGTTGGAACAATAGCAATGGAGTTAATCAGAATGATCAAACGTGGAATC10.9900990099009901No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT10.9900990099009901No Hit
GTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTT10.9900990099009901No Hit
CTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAG10.9900990099009901No Hit
GATCTGGGTGGAAGCGCGCTTGTTCGCAGGGGACTGGATCCGAGAATGTG10.9900990099009901No Hit
GGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATC10.9900990099009901No Hit
GCCTCAAGAGTTGGTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACA10.9900990099009901No Hit
GAGAACATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTG10.9900990099009901No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC10.9900990099009901No Hit
GTATACGAAACCTCTAATCTGCAGCCCAGGTGTTGCGATAGCCCTTCTTT10.9900990099009901No Hit
ACACAACTGGAGAAGGGGTGGACTCGAGTAGCGACCTCAGGTGTTCCAGC10.9900990099009901No Hit
GGGCGGGTGGGGGTGGGGGGGGGGGGGGGTTGGGGGGGGGTGGGTGGGGG10.9900990099009901No Hit
CCCTCAGAGCCGCATTCTTTGGGGGCAGCCAAAGAGGCTGAGTCCTGATT10.9900990099009901No Hit
CCTATATGATAGAACCAATGGCACCACAAAAATCAACAACAAAAGGATCA10.9900990099009901No Hit
TCATCTTTCTCACAACATTTGCCAGTTTGGCCTTCTTTTCATTGCCCCCT10.9900990099009901No Hit
ATCAATGACCGAAATTTCTGGAGGGGTGAAAATGGCCGAAGGACAAGGGT10.9900990099009901No Hit
ACCACTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTG10.9900990099009901No Hit
CCTCAAGAGTTGGTGCGTGGGGTTCAGTCGCTGGTTTGCCCTATTGACAA10.9900990099009901No Hit
CAAGGAGAGTGTCGTTGGTGATGTATTGTTGAACAGCGTCATTCTAGACA10.9900990099009901No Hit
AAACAGGAGGACCCATATATATAAGATTAGACGGAAAGTGGATGGGAGAA10.9900990099009901No Hit
GCTTGTATTTGTGTTCGAATCTTCATTCTTTTACTCTCGAACATGTACCC10.9900990099009901No Hit
CCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCAAAGACT10.9900990099009901No Hit
TTGTCGATGGATTCAATTACCCAGTTCTGGAAAAGCACTTTTGCATCTTT10.9900990099009901No Hit
GTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATCCTTACTCCTCCA10.9900990099009901No Hit
GCAGAGGAGGGAAGCGGGTGCGTCCCAGCGGCGGGCGTTCCGCACGAGGG10.9900990099009901No Hit
TCGCAAATGCGACCTTCCTTTTATTATGCACCCATACAGGTTTGTGTTCC10.9900990099009901No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10.9900990099009901No Hit
GAGGGGTGCCTAAACATTCTGATCAGTGCCCCACGACTGGGCGCCAACAC10.9900990099009901No Hit
CCCTTAAACAGGCTCACAGGGAGGAGCCAGCGCCCCGAACTCTCGCCACC10.9900990099009901No Hit
GGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCTGATAC10.9900990099009901No Hit
GATCATGATCACAGAGATCACATCTTTCGGGATCCGTCCAGACACGAAGT10.9900990099009901No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT10.9900990099009901No Hit
AGTTAAATTCATTATTTTTGCCGTATGAGATCTTCAATGGTGGAACAGAT10.9900990099009901No Hit
GTCCGGCGTGCGGCCACGGAGAATGTGGCGCCCCGGGACTCCCGGGCCCG10.9900990099009901No Hit
GTATACTAAACCTCTAATCTGAATACAAGGTGTTGCGATAGCCCATATTT10.9900990099009901No Hit
CCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGT10.9900990099009901No Hit
ATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATG10.9900990099009901No Hit
TCCCTGAACACAGTAGGCACTTAATTCAAATTTGCTGAATGGATCAGTGG10.9900990099009901No Hit
TAAGGACACTGTTCCAGCAAATGCGGGATGTGCTCGGGACATTTGACACT10.9900990099009901No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG10.9900990099009901No Hit
CGATTGAACATATCGCAAGCATGAGGAGGAACTATTTTACAGCAGAAGTG10.9900990099009901No Hit
GGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCAT10.9900990099009901No Hit
TCCCATCACATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAAGCACTT10.9900990099009901No Hit
TATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTT10.9900990099009901No Hit
ATTTTGACGTTTGCGATTAGTGTATTACTTTGGTCAGGAATGTGAGTCTA10.9900990099009901No Hit
ATTCTCCACTTAGAGAGGCTTCCCTTGCCCAGTTCCGCTGACGCCGGGCC10.9900990099009901No Hit
GTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACA10.9900990099009901No Hit
GTATGACAATTGGAATGGCTAACTTAATATTACAAATTGGAAACATAATC10.9900990099009901No Hit
CACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGAGGCTTTTTCCAAACTG10.9900990099009901No Hit
TGGGGGTGGGGCGGGGGCGTGGGGTGGGGGGGGGGGGGGGGGGGGGGGGG10.9900990099009901No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10.9900990099009901No Hit
GCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGC10.9900990099009901No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers