FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTTGCTCTATCTTGAGGACCCCACAAAAACATATGCATCTCCATTCT11.7543859649122806No Hit
GAAGAAGTCAGGCCCAGAGCACTGAAGCCATCAGACGGCAGGTTGTTAGA11.7543859649122806No Hit
GAATACTGGAGAATTGCCTCTGACGAGTATCCTCCACCCTGATCCTCTCA11.7543859649122806No Hit
GCATGAGGAGGAACTATTTTACAGCAGAAGAGTCCCACTGCACGTCTACT11.7543859649122806No Hit
ATTAGAAACTGACCAAATAATAAAACTTCTACCATTTACTGCTCCCCCAC11.7543859649122806No Hit
GCCTTGCTCAATGCATCCTGTGCAGCCAGGGATGCCTTTCTGCTGATCCC11.7543859649122806No Hit
AGATTAGAAGCGGCAGTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG11.7543859649122806No Hit
GCTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTG11.7543859649122806No Hit
GCGATATGGTCACATGTGGATGGTTTTTACATGTTTTCGGATGACCATTT11.7543859649122806No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT11.7543859649122806No Hit
GGTGTGTTGTACTCCTGTGTTCATAGGAGGATTCTTCACGATAACCTAAA11.7543859649122806No Hit
GTATGGATAGCAAATAGTATCATTGCCACAACTACTTCAGTGCATGTGTG11.7543859649122806No Hit
ATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGTCT11.7543859649122806No Hit
AAAATACTGTGTTCTTGAAATAGGAGACATGCTCTTGAGGACTTCGATAG11.7543859649122806No Hit
GATCATGGGCCTCTAGACTAGGGATAACCAAGTCCAAAACCCCATTGCTC11.7543859649122806No Hit
GTCCTAGGATTAATATTTTAATGTTAAACATGCTAATAAAGGTATTGTTA11.7543859649122806No Hit
ATACTACGTTACCTCGTTTCTCTCAAACCCTTAAAAATCGTTCAATACTC11.7543859649122806No Hit
TACTGGTGATTCAGATACAGCTGGTACAGGGGGCCCGCTTGAAGGGATCC11.7543859649122806No Hit
CTCATGGACCAAGCAACGGCACTCTGGGGAACAAACAGGACACAAAAACT11.7543859649122806No Hit
GAGTTAGTGATAAATGTGCCATGCTAGTGTGGAATGGGTGCTGCCCAGTT11.7543859649122806No Hit
TCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGA11.7543859649122806No Hit
GCATTGGAGTTGCTCGAATAAATGAATCAGCTGAAATTATTATTGGAGTA11.7543859649122806No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT11.7543859649122806No Hit
ATATTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTGGGTCG11.7543859649122806No Hit
GAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGG11.7543859649122806No Hit
GTCAAAGAGAATCATTTCGTCAATAAGAAATAGAATAAAATACAAATCAA11.7543859649122806No Hit
AATATGGTAGTGTACAAAAATACGGCATTTCTCAATCCATAAGGATCAGC11.7543859649122806No Hit
CAAGACTACCCGCCACTGGAAGGACAAATCTTCTGGCTTTGCACTTTCCA11.7543859649122806No Hit
CTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCATTCA11.7543859649122806No Hit
ACAATAGAGAGGATGGGGAGTTCTGCTTTTGATGAGAGAAGAAATAAATT11.7543859649122806No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA11.7543859649122806No Hit
GCATTGAGCAAGGCCGAAATTATGTACACTCCCTTCATTATGTATTCAGT11.7543859649122806No Hit
CTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCA11.7543859649122806No Hit
GGTCAATGATGACTAGCATTTCTGCAGGTATTTGACTTCGAATCTTCATT11.7543859649122806No Hit
ATAGAGCATTCCGCACCTACACTGGGTGAATATCCGATTGGCCATGTTTC11.7543859649122806No Hit
TATAGGAACCTGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGCGCAT11.7543859649122806No Hit
ATATAAATAAGACAGGGACATTTGAATAAACAAGCATTTTTTATCGCTAT11.7543859649122806No Hit
TGGCCACAACTACGGACGGGCAGGGGGGAGGAAGGAGTGGACCCAAGCTG11.7543859649122806No Hit
TGATTACATATATCACCAGAACTCAAACCGAGTGGTTGAGAAACATTCAT11.7543859649122806No Hit
TACTAAAGATACACAGATAGTTTCAATAGTTGAGAAACACAAATTCAGAC11.7543859649122806No Hit
GAGCAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11.7543859649122806No Hit
TCATGATAAAGGAAGGTAGCCGCGAACTGAACTATGTAAAGAGCGCAAAC11.7543859649122806No Hit
TACTTGAAGAATCCCACCAAGGAATATTTGAGAATTAATGCCTTGAAACA11.7543859649122806No Hit
CAAAAGCATGAAGACATTCTTTGGCTGGAAAGAGCCTAACATAGTCAAAC11.7543859649122806No Hit
TATCTACCCTTGTTTGTTGAACAACTTCCATTGTTTCAAGGCATGAATTC11.7543859649122806No Hit
AAGAAGGGCTATCGCAACACCTGGGATGCAGAGTAGAGGTGTCGTATACT11.7543859649122806No Hit
CTAAAACATCATAGATGACGAATAAGTTATTGACAACTTCTCACTTCCAT11.7543859649122806No Hit
GATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGACCCGAGACT11.7543859649122806No Hit
GTGGCACATTTCAAAGCGAGGTGCTTATGGGTCTGCTGACTCTGATGCTC11.7543859649122806No Hit
CACAGAATGTGAAACAACAGTTTATACGAAATGTCACACAAGGAGAAAGC11.7543859649122806No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT11.7543859649122806No Hit
CGAAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTAT11.7543859649122806No Hit
TCACAAGACACAGAGATTTCTTTCACAATCACTGGGGACAACACTAAGTG11.7543859649122806No Hit
AAACAAAGAAGAAAATTGAGAATATAAGCCATCTTGTAATAGATGAGAAT11.7543859649122806No Hit
GAACAAGCTTTTAGAAAATAATCCAAGCAAAACTATCTGCAACCTATGCG11.7543859649122806No Hit
GAAATAGGGACAAGTAGAAAAGCTGGGCAAATAATGAAGGACGACAACAC11.7543859649122806No Hit
GATCAGGGAGAAGATTACATATATCAACAGAAATCTACCTGAGACATTAA11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAATT50.0145.09
GGAGAAT50.0145.08
TGGAGAA50.0145.07
CCCAAGC50.0145.0145
CTGGAGA50.0145.06
TACTGGA50.0145.04
AATACTG50.0145.02
GAATACT50.0145.01
ATACTGG50.0145.03
ACTGGAG50.0145.05
Produced by FastQC (version 0.11.5)