FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_B02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_B02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC100.6389776357827476No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG40.25559105431309903No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40.25559105431309903No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG30.19169329073482427No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA30.19169329073482427No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT30.19169329073482427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.19169329073482427No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG30.19169329073482427No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30.19169329073482427No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC30.19169329073482427No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGT20.12779552715654952No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA20.12779552715654952No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT20.12779552715654952No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT20.12779552715654952No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20.12779552715654952No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC20.12779552715654952No Hit
CATTAGCTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCAAT20.12779552715654952No Hit
TATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGAGCATGGCAC20.12779552715654952No Hit
ACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAG20.12779552715654952No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA20.12779552715654952No Hit
CAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAA20.12779552715654952No Hit
GAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACA20.12779552715654952No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA20.12779552715654952No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA20.12779552715654952No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA20.12779552715654952No Hit
GAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAA20.12779552715654952No Hit
CTCCTAGCTAAAGTTTCAACAAAGTATACGAAACCTCTAATCTGCATCCC20.12779552715654952No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC20.12779552715654952No Hit
GCAGATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAA20.12779552715654952No Hit
GGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAAT20.12779552715654952No Hit
GAGAAAGATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAATCA20.12779552715654952No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG20.12779552715654952No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT20.12779552715654952No Hit
GTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTT20.12779552715654952No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20.12779552715654952No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT20.12779552715654952No Hit
ACATTTGCCAGTTTGGCCTTCTTTTCATTGCCCCCTACTGGGAGCCCAGA20.12779552715654952No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA20.12779552715654952No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT20.12779552715654952No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG20.12779552715654952No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.12779552715654952No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20.12779552715654952No Hit
GGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAGAAC20.12779552715654952No Hit
CCATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTT20.12779552715654952No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT20.12779552715654952No Hit
CTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTA20.12779552715654952No Hit
ATTTCATCTTGATCTTGGAGGTTCCATTGGTTCTCACATATAGGAACATG20.12779552715654952No Hit
GTATTCTTGCCCCCACTTCATTTGGGAAAACAACTTCCATAATCACATCC20.12779552715654952No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG20.12779552715654952No Hit
CATATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGC20.12779552715654952No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA20.12779552715654952No Hit
CTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATA20.12779552715654952No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATCC20.12779552715654952No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA20.12779552715654952No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC20.12779552715654952No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG20.12779552715654952No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA20.12779552715654952No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA20.12779552715654952No Hit
GAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGG20.12779552715654952No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)