FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGATAGACTGGGGCATCCAGATTTTGCCAGTAAGGGACTGCCCCCTGT15.0No Hit
AGAACTGAGATGTCGCTGATAGGGATAAGAGTCAGCAAAATGGGAGTAGA15.0No Hit
GTGTTTGGGAGTTCTCTCTGGGACCTCTGCGTATCCAGGCCATGGCTCTG15.0No Hit
CCCCTCTCTGGCCCCCTGCAGCCGGCACTCTGTTTCTGTGAATTTGACTT15.0No Hit
GCTAGTCTCACAGGAGGATTGCATGATAAAGGCAGTTATGTGCGATATGA15.0No Hit
CAGACAGAGATCACAAGGAGGCAGAGAGGCAGGCAGAGAGAGAGGACGAA15.0No Hit
GATCTGGGCTGAGCTGACAGCAGTCAGCAGGAAAGGCAGGAGCAGGGCCC15.0No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC15.0No Hit
CATTTTGCTGGCAGGGTGTGCAGTGGGTGGGAGGGGATCAGTTGGTGATA15.0No Hit
CTCATAAGAAAAATTCGAAGAAATTATATAGAGACACTTTGAAGAGCTGC15.0No Hit
GAAGCAGACTTTCCACTGAGCAGGGAGCCCGATGTGGGACTTGATCCCAG15.0No Hit
GGGTGGGTAGGAGGGGGGCGAGTGGGGGCGGGGGGGGGGTGGGGGGCGGG15.0No Hit
GTGAAACGCAAGGAACTGAGAAGTTGACAATAACTTATTCGTCATCAATG15.0No Hit
CTTTTACTCTCGAAAATGTACCCTTTCCCTAGTCTGGCCATTTTTGTTGA15.0No Hit
GTGGTTACCTTTGATTCTGTCTCTGTGGAAGGTTCTCTTAGATAGGTGTC15.0No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA15.0No Hit
AAATTCCATTTTGTTGTATAACATTGTGGGATCTTGTGACCATTGAATTT15.0No Hit
TATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTT15.0No Hit
CATGACCCTGAGATCAAGACCTGAGCTGAGATCAAGAGTTAAGACGCTCA15.0No Hit
CCTATATCAACTGCTAGTGAAAAATTTCTCGAATAGATTGCAGAACTTAT15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers