FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_A07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_A07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC40.24737167594310452No Hit
GAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAG40.24737167594310452No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30.1855287569573284No Hit
GGGCAAGGGTTAGGTCCAAGTGGAATAGTGGCAGGGTGAGTCCCCAGGTG30.1855287569573284No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC30.1855287569573284No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA30.1855287569573284No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC20.12368583797155226No Hit
GTGAAGGAGTGGTCTTCTTGCCAGCTGGGAGGGAGGAGCAGTGGAGAGGC20.12368583797155226No Hit
GTGCAGAGTGCGGACCAGGTCCTGGTGCTCAGGCAGGGTGAGCTGCGGGA20.12368583797155226No Hit
GGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAAGAGAA20.12368583797155226No Hit
TGTTTATTCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAAT20.12368583797155226No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT20.12368583797155226No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.12368583797155226No Hit
TTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCA20.12368583797155226No Hit
GCTATTCTCAGAAAGGCAACCAGGAGATTGATCCAGTTGATAGTAAGCGG20.12368583797155226No Hit
CCTCCAGCAAGGAAGGGATAAGTACTACAACAAGGCGGGTCCCAGTGAGT20.12368583797155226No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG20.12368583797155226No Hit
GGCTGGGAGAATGCTGGCTGTACGGAAAGGCTGGAGCTGACCACGTGGTG20.12368583797155226No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC20.12368583797155226No Hit
TAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGT20.12368583797155226No Hit
CTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATT20.12368583797155226No Hit
GTTGTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCC20.12368583797155226No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC20.12368583797155226No Hit
CTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTAC20.12368583797155226No Hit
GAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATT20.12368583797155226No Hit
GCCCAAAGGGTAGTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAG20.12368583797155226No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20.12368583797155226No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC20.12368583797155226No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA20.12368583797155226No Hit
GAGATAGGGCGAAGGGAGGGAGAGACTCTGAGGCAGAGGACTCTGTTGTG20.12368583797155226No Hit
CCTTATTTTAACTTGATTTCTGAAGTGGACAGGGCCGAAGGTACCATGTT20.12368583797155226No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG20.12368583797155226No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20.12368583797155226No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT20.12368583797155226No Hit
ATACACACACACACACACACACACACACATTCAGAAAACTGAAAGAAGAT20.12368583797155226No Hit
GCCTAAGGCAATCTCTAGATAGTAGCCTGGGATTCAGACCCAGATTTGTT20.12368583797155226No Hit
CCACAGCTTCTTTAACTCAAATGATCTTCTCGTCTGAATTTGTGTGTCTC20.12368583797155226No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC20.12368583797155226No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT20.12368583797155226No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)