FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGGAAACTCGCAAAAACAACCACAATGTGCATAAAGACTTACGAATC13.0303030303030303No Hit
TCGTATATGACTGAACGTATGGAAGCTGCAAGAAGATGAAGAAATCGGCC13.0303030303030303No Hit
CCCTAGAAGAGAATCCCAGTGCTGGGAATGACCCTAAGAAAACAGGAGGA13.0303030303030303No Hit
CAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCA13.0303030303030303No Hit
AGTCCTATTTGGTGACCATATACTCTCCAGTCAATTAAAAAATATGAATG13.0303030303030303No Hit
GTATATGGAGAGAATAAAAGAACTCAGAGATCTAAACTCGCAGTCCCGCA13.0303030303030303No Hit
ATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGGACAT13.0303030303030303No Hit
GCTGAGACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGT13.0303030303030303No Hit
CTTCTAATAGATGGCTCAGCATAACTGAGTCCTGGCATGATGATGGGCAT13.0303030303030303No Hit
AGCGAATGTCGCAGTCCCGCACTAGAGGGATACGCACTAAGACCACTGTG13.0303030303030303No Hit
GTTCAAATATATTCAATATGTAGAGAATAAAAGAACTGAGAGATATAATG13.0303030303030303No Hit
CAAGAGAATAAGAGTCAAATCTCCAGAATACAATTACAACAACGCAACCA13.0303030303030303No Hit
TACATCCTCAGTGAAAGACCATAGTATTATCAAGGTCTCTTATATGTTAA13.0303030303030303No Hit
CTCCAACCCAGATGTGCGTTCATCAGGATCTTCAGTCATTGCACCAGCAA13.0303030303030303No Hit
TCCCTTGGGTTAAGTGAAACACTTCAATATAAACACTGCATGTTCCGCCG13.0303030303030303No Hit
TCCCTTGGGTTAAGTGGACCACTTCAATATAAACACTGCATGTTCCGCCG13.0303030303030303No Hit
GTTTTCATTCAAATACGGCAATGGTGTTTGGATAGGGAGAACTAAAAGCA13.0303030303030303No Hit
ATAGTAGAAACAAGGCAGTTTTTCATGAAGGACAAGTTAAATTCATTATT13.0303030303030303No Hit
CAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGTTGA13.0303030303030303No Hit
ACCGACATGGGAATGGGGACCCGCACACCATGGAGATAGCCGTTACACTA13.0303030303030303No Hit
GTCCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGAC13.0303030303030303No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG13.0303030303030303No Hit
GTATACGAAAACGCGAATCTGCCTCACAGGTGGTGCGATAGCCCTTCTTT13.0303030303030303No Hit
GACCCAGTAAACATAAAACAACAATACTCAGAAAAGGGAAAGTGGACGAC13.0303030303030303No Hit
GCCTAACATAGTCAAACCACATGAGACAGGCATAAATCCCAATTACCTCA13.0303030303030303No Hit
CACAATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCC13.0303030303030303No Hit
ATTAATATACAGGTCTTACCACCAGCACGTGCCACGCGGGTTCAAGACTT13.0303030303030303No Hit
CCTATATGAAATGAGAGTGAAAAATTTCGCGAAGAGATTGCAGAACTTCT13.0303030303030303No Hit
AATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCA13.0303030303030303No Hit
GTCTGTATGACAATTGGAATGGCTAACTTAATATTACAAATTGGAAACAT13.0303030303030303No Hit
TTTTTGGAGAGTATGGATAGCAATTAGTTGCATTGCCACAACTACTTCAG13.0303030303030303No Hit
GCGGTGGGGGCCGGGGGTGGGGGGGGGGCCGGTCGGGGGAGGGCGGCCGG13.0303030303030303No Hit
GAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACCCCTCATGT13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)