FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_A04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_A04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT32.112676056338028No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT21.4084507042253522No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG21.4084507042253522No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAG10.7042253521126761No Hit
GTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGA10.7042253521126761No Hit
GGACAGATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAA10.7042253521126761No Hit
CAACAAACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTG10.7042253521126761No Hit
CTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCGTAAGGCCA10.7042253521126761No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10.7042253521126761No Hit
GACAGAAGACTCGGCCTCAATCATGCTCTCAATCTGCTGAAGAGACTGAA10.7042253521126761No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA10.7042253521126761No Hit
TACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTG10.7042253521126761No Hit
GAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGACGCTATCTAATA10.7042253521126761No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT10.7042253521126761No Hit
GTTTATCATGGTGGGGGTTGTGGGGGTTGCTGGGGTAGGGGTGGCGGGTG10.7042253521126761No Hit
AATGTCAAGAGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCC10.7042253521126761No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10.7042253521126761No Hit
ATTGGGATCAGCTGAAAGTCCTCAATGGCTGCACAGGAGGCATTGCGCAA10.7042253521126761No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGCCGATTTTGCTCTCAT10.7042253521126761No Hit
GAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTCACTT10.7042253521126761No Hit
CCACATTCCCTTATACTGGAGCTCCTCCATACAGCCATGGAACAGGAACA10.7042253521126761No Hit
TCCCAGTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGG10.7042253521126761No Hit
TAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGCAA10.7042253521126761No Hit
TCATTGATCCCGCTGAATGCTGCCATAATGTTTGCTCTTTCAAAAGTGAG10.7042253521126761No Hit
AATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGCT10.7042253521126761No Hit
ACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATC10.7042253521126761No Hit
GGTTTAGAACTGAATGATGTTGTGTGTGGTGACAGTGAGGGACGTGCGGT10.7042253521126761No Hit
CTCCTAATTGGAAATCACTATTCTAAAATGTAGAGACAAATGATATAACC10.7042253521126761No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG10.7042253521126761No Hit
TTGTCAATAGGGCAAACCAGCGACTGAACCCCATGCACCAACTCTTGAGG10.7042253521126761No Hit
CACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGT10.7042253521126761No Hit
CTCGCGGGATCCGCGCGGTGGCCACTGGGGGCGGGGTGGGGCGGGGGGCT10.7042253521126761No Hit
GTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGATTAC10.7042253521126761No Hit
GATAAGTGTGGACTAGCTCTCACTCAGGGCCCTTGCTCTCGCTCATCATT10.7042253521126761No Hit
ACTCCTGGAGTGAACAACTGATCCCAGCACGTCCCTTGGGTTAAGTGCAA10.7042253521126761No Hit
GGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACT10.7042253521126761No Hit
GAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCT10.7042253521126761No Hit
TTGCAGGAAAGAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACA10.7042253521126761No Hit
TCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGA10.7042253521126761No Hit
GATATCTAATGTCGCAGTACCTCACTCGCGAGATACTCACTAAGACCACT10.7042253521126761No Hit
GTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAGAT10.7042253521126761No Hit
GTAGAAGCTTGGGGGATCTTGTGGGGCGGGAATTTTCACAATTTCCCCGT10.7042253521126761No Hit
CAGGTAGATATTTAAAGATGAGTCTTATAACCGAGGTCGAAACGTACGTT10.7042253521126761No Hit
TAGCTCTTGTCTGCTGTGGCGAAAATGGGCAATCCTCGCAGGACAGCGGG10.7042253521126761No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC10.7042253521126761No Hit
GAATAGATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCC10.7042253521126761No Hit
GCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGCGATAATCAAACT10.7042253521126761No Hit
TTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGA10.7042253521126761No Hit
CACCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG10.7042253521126761No Hit
CTGCTGCTCTTCTTACTATGTTTCTAGCAGCGATAATCAAACGTTGGTCA10.7042253521126761No Hit
ATTCTCAGAAAGGCAACCAGGAGATTGATCCAGTTGATAGTAAGCGGGAG10.7042253521126761No Hit
TCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCA10.7042253521126761No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG10.7042253521126761No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG10.7042253521126761No Hit
TCTTGGTCATGTTGTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTT10.7042253521126761No Hit
GGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGTACATCTGTTCAAGA10.7042253521126761No Hit
CATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT10.7042253521126761No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC10.7042253521126761No Hit
AGACCAACCCACAGTGTCACTGTTTACCCCACAAAAGGATATGCTGCTCC10.7042253521126761No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT10.7042253521126761No Hit
TTTCATCAAGTTCTATCCAGCTTGAATCAGTCAATTCACATGCCTTATTG10.7042253521126761No Hit
GTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGACCGTACT10.7042253521126761No Hit
GTTCATGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10.7042253521126761No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG10.7042253521126761No Hit
CCTATATGTGAGAACCAATGGACCCTCCAAGATCAAGATGAAATGGGGCA10.7042253521126761No Hit
GGGGTAGTCAGGTGGGTCGGGGGGGGAGTCAGTGACCGGAATTTCTGGAG10.7042253521126761No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT10.7042253521126761No Hit
ATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAGATGATGACTAAT10.7042253521126761No Hit
ATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTT10.7042253521126761No Hit
GGGAGGGGCGGGGCGGGGGGGGGGGGCGGGGGCGGGGGGGGGGGGGGGGG10.7042253521126761No Hit
GGGAGGGATTACCATTATATGCTGCAATCAAATGCATGAAGACATTCTTT10.7042253521126761No Hit
GCGGGGGTCCCGGGGTGGTGTTTGGGCGGGCCGTGCAGCTGGGTTGGGGG10.7042253521126761No Hit
ATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGAC10.7042253521126761No Hit
ATGTTCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATG10.7042253521126761No Hit
AACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCACACCCAG10.7042253521126761No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT10.7042253521126761No Hit
AATAAACAGATCAGGGAACGGGGCCTACATTCACATACACTGGTGAACTG10.7042253521126761No Hit
GTCAAGAAAGAAGAAGAAGTGCTAACGGGCAACCTCCAAACACTGAAAAT10.7042253521126761No Hit
GAAACAGCCACTCTTGTTGGGAAACAAATCGTGGAATGGATCTTGAAAGA10.7042253521126761No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC10.7042253521126761No Hit
CACAGAGCCTGGTGCACCCCAGCTCACCCCGATTGATGGACCACGACCTG10.7042253521126761No Hit
GGGTAGTGGGTGGTCGGGATTGGCGCAGTCATGTTACTCCAATGCCGCCA10.7042253521126761No Hit
GACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACTCTCCTT10.7042253521126761No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC10.7042253521126761No Hit
GTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACCCCTCTTGTGG10.7042253521126761No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC10.7042253521126761No Hit
GAAGTTGTAAGGCTTGCATAAATGTTATTTGTTCCAAACTATTCTCTGTA10.7042253521126761No Hit
GAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAAGAGAACAAGCCA10.7042253521126761No Hit
TCTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATTTAATGTCA10.7042253521126761No Hit
CATGAAGACATTCTTTGGCTGGAAAGAGCCTAACATAGTCAAACCACATG10.7042253521126761No Hit
AAGCTGGGTTGATACATGCTAAAATTAGCAACAAATCAATAGCGATAAAA10.7042253521126761No Hit
TCTCTGTGTCTTGTGAATTAGTCATCATCTTTCTCACAACATTTGCCAGT10.7042253521126761No Hit
ATACTTAATTCTGGGCGATATCTCTTGGCGTCTTTGCCCAAAAAGAGCTA10.7042253521126761No Hit
CACCCAAGGGTGCTATAAACACCAGCCTCCCATTTCAGAATATACATCCG10.7042253521126761No Hit
GCATGTCCTCATGCGGGAGCAAGAAGCTTCTCCAAAAATGTAATATGGCG10.7042253521126761No Hit
AAAGCAGGTAGATATTTAAAGATGAGTCTTCTCACCGAGGTCGAAACGGA10.7042253521126761No Hit
GTCGGTGTTTTTTCTTGCCTATTGTTCTTTGCGTGACCCTCTTGTTGGTC10.7042253521126761No Hit
GTTCACTCGATCCAGCCATCTGTTCCATAGCCTTTGCCGTAGTGCTAGCC10.7042253521126761No Hit
GATGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAA10.7042253521126761No Hit
CTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAATTCCTTGGTCA10.7042253521126761No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCC10.7042253521126761No Hit
GGGGCAGCGGCAGGCAGAGGGAGAAGCAGGCTCCCCACCAAGCGGGAAGC10.7042253521126761No Hit
TATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCAC10.7042253521126761No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC10.7042253521126761No Hit
ATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATC10.7042253521126761No Hit
GGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCTCTTCTCCCA10.7042253521126761No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT10.7042253521126761No Hit
TCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAA10.7042253521126761No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA10.7042253521126761No Hit
GTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCT10.7042253521126761No Hit
GTTCTGGAAAAGCAGTATTGCATCTTTTTTGAAATGCCTCAACAGTTGGT10.7042253521126761No Hit
ACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACAC10.7042253521126761No Hit
GTAGAATCTGGTGCCCCGAATGCACTATTGAAGCACCGATTTGAGATAAT10.7042253521126761No Hit
GTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATC10.7042253521126761No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT10.7042253521126761No Hit
ATTAAGGTCAATGCTTGCTATCATTTCTGCTGGTATTTGTGTTAGAATCT10.7042253521126761No Hit
GGACTGTTGATTACAGAGGTTGCCGGGAAGGTCCTGGGCGCGTTCCCGAT10.7042253521126761No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA10.7042253521126761No Hit
TTAGGGGAGGCGGTAGTTTCTCGAGTGTGTTCGGTGTGGCGGCATGGAGG10.7042253521126761No Hit
GTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCT10.7042253521126761No Hit
TAACATGGAGGAATAGGAATGGCCCAACAACAAGTACAGTTAATTACCCT10.7042253521126761No Hit
GTACAGTGGATTCGTAAGGACACTGTTCCAGCAAATGCGGGATGTGCTTG10.7042253521126761No Hit
AAGAAGGGTGTTAATCAGAATGATCAAACGTGGAATCAATGACCGAAATT10.7042253521126761No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGTGGGGGGGG10.7042253521126761No Hit
AGGTTTCTCCCAGTAGCCGGCGGAACAGGCAGTGTTTATATTGAAGTGTT10.7042253521126761No Hit
CACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATC10.7042253521126761No Hit
TATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTT10.7042253521126761No Hit
TCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCAT10.7042253521126761No Hit
GAAAATATGAGTACTTGAAGGGGATGAAGAGTTCACCAGGGTGGGGAGAA10.7042253521126761No Hit
AATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAAG10.7042253521126761No Hit
CTGCGATGTGCGCGCTGGTGGCTGTGTGGGGCGTGTGGTCGGCTGTCCCT10.7042253521126761No Hit
GCCAAGTGTCGAGGCCCATGTTCCTATATGTGAGAACCAATGGAACCTCC10.7042253521126761No Hit
TAAATATATACTTGAACAGAAGTTAATGCAGAAAAATCTCAAACTATAGA10.7042253521126761No Hit
TATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTT10.7042253521126761No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTAGCCACTAATTGATGGCCAGC10.7042253521126761No Hit
GAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAATTAG10.7042253521126761No Hit
GGTCCAAAATGAATTCAATAAGGCATGTGAATTGCCTGATTCAAGCTGGA10.7042253521126761No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAG10.7042253521126761No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers