FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R2_A03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R2_A03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTAAGTACAATCTGAACCATGATCTCAAATTAAGTAATTAACAAACGA12.631578947368421No Hit
ATCCACGCCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAAT12.631578947368421No Hit
ATGCTAGAGTCCCGTTTTGGTTTAATTTACAACACTACGTCCCCGTGGCC12.631578947368421No Hit
TAGATGGTGCTCCAAGGGACCAGCAGGAGGGATCAGCACCACATGGAGAA12.631578947368421No Hit
GGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGCACACAACT12.631578947368421No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG12.631578947368421No Hit
TAATACGTCCCATGCTCAGGATGTTTCTGAACCCCTCGGGTTGATTTCTG12.631578947368421No Hit
GCTGGAGTCAATTCCGCAGTTCTGGAAATGCACATTTTAATCTATTTGTA12.631578947368421No Hit
CATAGGATCTAAAGTTTTCAAGGCTGGGGAAGTTCTGAGCTAGACTTTGG12.631578947368421No Hit
GACCCGAACAACATGGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAG12.631578947368421No Hit
CTTGCGATATGTTCAATCGGGGCAACATCTTCTCCTATTTCATCAAGTTC12.631578947368421No Hit
AAATTAAACAGTATTCAAGTTATCAGAAAGATCACAAATATCTATCAAAC12.631578947368421No Hit
CAATGCTTCTTGTCCAGCAATTGATGACTTTAAGCTGGTGCCAATGATAA12.631578947368421No Hit
CCCATACACACAAGCACGCAGACACAAATTAAGTGCAACTGATCAACTCA12.631578947368421No Hit
CAAAATGTCAAAATAAGTGAACTCCAAAATTGAACAATACTTGAGGAAGC12.631578947368421No Hit
TTGAAGTACTTCAGGTCAATGCTTGCTAGCAGTTATGCTGGTATTTGTGT12.631578947368421No Hit
GAGTATTGGAGTAACAGTGATAAAGACCAACATGATAAACAATGACATTG12.631578947368421No Hit
ATTCACAAGACACAGAGATTTCTTTCACAATCACTGGGGACAACACTAAG12.631578947368421No Hit
ACCTAGAGAAAGCCAACAAAATAAAATCTGAGCAGACACACATTCCCATC12.631578947368421No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12.631578947368421No Hit
CTCACGACCCCTCCCCTGGCGCAACAGACGTGGGCAAAGTTCGGATTTCT12.631578947368421No Hit
CCTACACCATGCTAGAAATTCCACCAGTTATCCTATATGAACTGGTAGGT12.631578947368421No Hit
TCCTTGAGATATATTCAATACGGGCAACATCTTATACAATTTCAAAAATT12.631578947368421No Hit
GTCAATTCACATGACTTATTGAATTCATTTTGGACCCAGCTTGATAGAGA12.631578947368421No Hit
TTATAGGTTCCATGCTCAGGATGTTTATGAACAACTCGGTTTGATTTCTG12.631578947368421No Hit
GTTCTCATATATGAACTGCTAGTGAAAAATTTCTCGAATAGATTTAACAG12.631578947368421No Hit
CAATGGCGCCTATATGTGTAACTGTAGTGTTTGATGAACATTAGCTGAGC12.631578947368421No Hit
CGTCTGGCCTAGACCAACCAACAGTGTCTCTGTTTACACCACAAAAGGAT12.631578947368421No Hit
CTGATTGAGTGTATGGGCTTGCAGTAGCTAGTGCGCATGACTTTGAAAGG12.631578947368421No Hit
GTCTTTGATGAACAATTGAAGAGACATCTCGGCCGTTGCAGTTACAAGCT12.631578947368421No Hit
CTTATACAATATACGCAATCTTCACATTCATGAATTCTGCTTAAAATGGG12.631578947368421No Hit
TATTTGGACAGTGTCAAATGTCCCAAGAACATCACTCATTTGCTGGAACA12.631578947368421No Hit
GTTTTCACGCTCACCTTGCCCAGATAGCGAGGACTGAAGAGTAGACGCTT12.631578947368421No Hit
ATTGGGATTTATGCCTTTCTCATGTGGTTTGACTATGTTAGGCTCTTTCC12.631578947368421No Hit
GAACATATCATCTTTAAAATAATAGAAATAGAAATTACACTCCTCTAATA12.631578947368421No Hit
GGGAAAGTGTGAAAATTCAAGGGTCCCACGAGCCCCCAATGTTATACAAC12.631578947368421No Hit
CAGTGATGCTGTGCCATATATTAGAAGAGGCCTTATTTTCTCAATTTTCT12.631578947368421No Hit
ATTCCTCTTGGGAATCCAGTAATTTGTAGTTGCAACGGCATCATATTGCA12.631578947368421No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)