FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S4_R1_H04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_H04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTTTCTCACAACATTTGCCAGTTTGGCCTTCTTTTCATTGCCCCCTA15.263157894736842No Hit
AGGGCTAAGTGGGGACTTGATCTGATCCTGCTCCGAGGATCGCACCCCGC15.263157894736842No Hit
CTTGTATTCCCTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCCTCG15.263157894736842No Hit
CATTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTC15.263157894736842No Hit
TCGAGAGTCTATGGCTTTCATTGAAGAATCCCACCCAGGAATATTTGAGA15.263157894736842No Hit
CTATTAGAAGAGGCCATATTATCTCAATTTTCTTCTATGTTGAGTAATTG15.263157894736842No Hit
TCCATGCCCCATTTCATCTTGATCTTGGAGGTTCCATTGGTTCTCACACA15.263157894736842No Hit
GTCTCAAGTGAAAAAGACAACATATAAATCACAACATTTTCCAGTTTGGC15.263157894736842No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA15.263157894736842No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC15.263157894736842No Hit
ACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAACAAAGCCT15.263157894736842No Hit
AGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGA15.263157894736842No Hit
AAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGT15.263157894736842No Hit
AGTACAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT15.263157894736842No Hit
ATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCACA15.263157894736842No Hit
GTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATG15.263157894736842No Hit
CCATACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAA15.263157894736842No Hit
GTTCATTGATGCTTAATGCTGGGCCATACGGCCAGAGGTGATACCATCAC15.263157894736842No Hit
CAATATACTTGTGGGATGGTCTCAAATCATCCGACGATTTTGCTCTCGTA15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)