FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S4_R1_G11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_G11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC32.5423728813559325No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT32.5423728813559325No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT32.5423728813559325No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA32.5423728813559325No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG32.5423728813559325No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC32.5423728813559325No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG21.694915254237288No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA21.694915254237288No Hit
CAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAG10.847457627118644No Hit
GTAAAAGAATGAAGATTCGAACACAAATACCAGCAGAAATGCTAGCAAGC10.847457627118644No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG10.847457627118644No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT10.847457627118644No Hit
GTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT10.847457627118644No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC10.847457627118644No Hit
AAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGA10.847457627118644No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG10.847457627118644No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC10.847457627118644No Hit
TTTTTAAACAATTCGCCACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.847457627118644No Hit
CTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGAC10.847457627118644No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCGTGC10.847457627118644No Hit
TGCCAGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCCGCTTGTCCTT10.847457627118644No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA10.847457627118644No Hit
CAAAAACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTT10.847457627118644No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG10.847457627118644No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA10.847457627118644No Hit
GATGATAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGG10.847457627118644No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT10.847457627118644No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG10.847457627118644No Hit
ATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA10.847457627118644No Hit
GGTCGGGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCC10.847457627118644No Hit
TTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTC10.847457627118644No Hit
GGGGACAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGC10.847457627118644No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT10.847457627118644No Hit
GGACTACACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTT10.847457627118644No Hit
CAGAAAGGCAACCAGGAGATTGATCCAGTTGATAGTAAGCGGGAGAGACG10.847457627118644No Hit
CACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGT10.847457627118644No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTGGTTTCATTACCAA10.847457627118644No Hit
TGATGATGTTGACCAAAGTTTGATTATCGCTGCTAGAAACATAGTAAGAA10.847457627118644No Hit
GTTCACCACATCAGTATCATTTCTCAAATGAGACCTTCCTTTTATAATGA10.847457627118644No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT10.847457627118644No Hit
GGACCTTACTGGCAAAATCTGTATTCAACAGTCTATATGCGTCTCCACAA10.847457627118644No Hit
GAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCC10.847457627118644No Hit
GGTCCTACATTTGCTTATCATTGGGATCAGCTGCAAGTCATCCATGGCTG10.847457627118644No Hit
CACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCA10.847457627118644No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10.847457627118644No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA10.847457627118644No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC10.847457627118644No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA10.847457627118644No Hit
CTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATC10.847457627118644No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG10.847457627118644No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG10.847457627118644No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT10.847457627118644No Hit
GTTCAGAAACATCCTGAGCATGGCACCTATAATGTTCTCAAACAAAATGG10.847457627118644No Hit
CATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGA10.847457627118644No Hit
AGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTACG10.847457627118644No Hit
CTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCTCTTT10.847457627118644No Hit
TTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGA10.847457627118644No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG10.847457627118644No Hit
GGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGC10.847457627118644No Hit
GAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAA10.847457627118644No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG10.847457627118644No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG10.847457627118644No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC10.847457627118644No Hit
GAGTTAAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTATTAGT10.847457627118644No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG10.847457627118644No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC10.847457627118644No Hit
GTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA10.847457627118644No Hit
GAAAAAAGAAGTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGC10.847457627118644No Hit
AATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAA10.847457627118644No Hit
CCTCAAGAGTTGGTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAA10.847457627118644No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA10.847457627118644No Hit
TGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCT10.847457627118644No Hit
GACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTT10.847457627118644No Hit
AATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAA10.847457627118644No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT10.847457627118644No Hit
CTACGTCCCCTTGCTCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA10.847457627118644No Hit
CTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGATTTATGCCTTTC10.847457627118644No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT10.847457627118644No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGGCCACTGT10.847457627118644No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC10.847457627118644No Hit
CTCACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCT10.847457627118644No Hit
GAATAATGGACATGATTCCAGAGACAACAAGGCAACCAAACGACTTACAG10.847457627118644No Hit
CATTCTTTTACTCTCGAACATGTACCCTTTCCCTAGTCTTGCCATTTTGT10.847457627118644No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT10.847457627118644No Hit
TTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCT10.847457627118644No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG10.847457627118644No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATAGAACAGA10.847457627118644No Hit
CATTCGGATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAGA10.847457627118644No Hit
GCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAG10.847457627118644No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC10.847457627118644No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT10.847457627118644No Hit
TATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCC10.847457627118644No Hit
CATTGATGCTTANNGCTGNNCCTCTTGNNTTCNNTNCCNAAAATGAGAAN10.847457627118644No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG10.847457627118644No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA10.847457627118644No Hit
GAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCC10.847457627118644No Hit
CATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10.847457627118644No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG10.847457627118644No Hit
GTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCAT10.847457627118644No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATA10.847457627118644No Hit
ATTACCGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACT10.847457627118644No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA10.847457627118644No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA10.847457627118644No Hit
CTCTTCTTTCTTCTCTTTTGTTATTGCCAGCTGTGACTCTGATGTCAGTA10.847457627118644No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers