FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_G09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_G09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34.545454545454546No Hit
GTATCATGTAATCGGATTTACATTTCATCGACGAACGGGCATAATCAATA11.5151515151515151No Hit
GAGTGGTTCAGAAACCTCCTGAGCAGGGCACCTATAATGTTCTAACAAAA11.5151515151515151No Hit
CAATATGGAGAGAATCAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA11.5151515151515151No Hit
GAGGTGTCGTCCTCAAGAAAGGTTCAATATTGGCCTTAACTTCATATGAC11.5151515151515151No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT11.5151515151515151No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT11.5151515151515151No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCA11.5151515151515151No Hit
GGGGGGGGGGAGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG11.5151515151515151No Hit
GTAAAAAATGATTCAGAAAGAAATCTCTGTGTCTTGGGAATAAGACATCA11.5151515151515151No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAACATGCATTAAGCAAAACCCAC11.5151515151515151No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGG11.5151515151515151No Hit
TCACCTCAGTTTTGCTCCAGGGTCATGACAGAAAGCTAGCCCGAAGAAGG11.5151515151515151No Hit
GAATAAAAGAAATTATAGATCTAATGTAGCAGTCCCGCACTCGCGAGATA11.5151515151515151No Hit
GATAAAGATTACACTTAAGTTCGCTTTCAGTAAGATGTTATTTTCCATGA11.5151515151515151No Hit
ATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACTCG11.5151515151515151No Hit
ATAATGAACCAAGTGGGTATGCACAAGTAAACAATGCTGTGGTAATGCCA11.5151515151515151No Hit
AACTTATACAATATACGGAATCTTCACATTAATGAAGTCTGCTTATAATG11.5151515151515151No Hit
GATTCCGTATATTGTATAAGTTTGGTCCTCAATCTGATACTAATAGCCCT11.5151515151515151No Hit
ATCCAATGCTCGGCGAGCTTGCGGAAACGGAAATGAAAGAATATGGGGAA11.5151515151515151No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11.5151515151515151No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA11.5151515151515151No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA11.5151515151515151No Hit
AATAAAATCTGAGAAGCCACACATTCACATCTTTTCATTCACTGGAGAGG11.5151515151515151No Hit
CGTTCACTCTTTGATCTATAACAATTAAAAATAGGTCAATACTCACTAAC11.5151515151515151No Hit
GTCCCGCACTCGCGAGATACTCACTAATACCACTCTGGCGCATATGGCCA11.5151515151515151No Hit
TTGTGCGACCATGCTTCAATCCTATGATCGGCGCGCTTGGGGAAAAGGCA11.5151515151515151No Hit
ATTGTCAACTACTCAGTTCCTTGCGTTTCACTGCCTTCTTCGGGAGACTA11.5151515151515151No Hit
GTTCATAGAAGCTTAATCCTGGGCAATATATCTTGTATTCATTGCACAAA11.5151515151515151No Hit
TTCATGCCTTGAAACAATGGAAGTTGTTCAACAAACAAGGGTAGATAAAC11.5151515151515151No Hit
TCCTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCGTGGGTGATGTGCA11.5151515151515151No Hit
GTACAGATGTTGCCCGTAAGGTACTGCACACTTTCGCCCTAGCGGGGTCC11.5151515151515151No Hit
CTACATGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC11.5151515151515151No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11.5151515151515151No Hit
GTCAGTAAGTATGCTAGAGTCCCGGTTTGGTTTCATTACCAACACTACGG11.5151515151515151No Hit
AAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGG11.5151515151515151No Hit
ATACTCACTAAGACCACTGAGGACCATATGGCCATAATCAAAAAGTACAC11.5151515151515151No Hit
AAACTTCTCAGTTCCTTGCGTTTCACTGACTTCTTCGTGAGAAAATAGTA11.5151515151515151No Hit
GATCAATCGTGGAATCAATGACCGAAATTTCTGGAGGGGTGAAAATTGAC11.5151515151515151No Hit
GGTAATTGTGTACGAATCTTCATACTTTTACTCTCGAACATGTACCCTTT11.5151515151515151No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCAAAGATTCAGTATAGAGACTC11.5151515151515151No Hit
GGCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAA11.5151515151515151No Hit
CAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGGAGTCT11.5151515151515151No Hit
AATAAGGCCTCTTATAATAGATGGCACAGCATCACTGAGTACTGGGATGA11.5151515151515151No Hit
CCTATATGAACTGCAATGGAAAAATTTCTGGTATAGAGTGCAGCACGGCG11.5151515151515151No Hit
GTCCAGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAAGGCCA11.5151515151515151No Hit
AAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATGAGACCAAATGAAAA11.5151515151515151No Hit
CTTGAGGACTGCGATAGGCGAAGTGTCGAGGCCCATGTTCCGATATGTGA11.5151515151515151No Hit
ATCAATAATTGTAGAATCTGGTGACCCGAATGAACTATTGAAGCACCGAT11.5151515151515151No Hit
ATCCACCCAAGATTTGCCTTCAACAGGATCTTCCGTCAATGCACATGCAT11.5151515151515151No Hit
CACATCAGGACGGCAAGAGAAGCGCCCCGCCCTCTGAATCAAGAGGATGA11.5151515151515151No Hit
CAGTAAACAGAACAAAACAATACTAAGAAAAGGTAAAGTGTACGAAAAAC11.5151515151515151No Hit
GATACACACCCTGGCCATCCAGATCTCAGTGCCAATGAGGCAAAGGAAGT11.5151515151515151No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAA11.5151515151515151No Hit
CTCGTATCTCTTGTTCTACTTCAAGAAGTAGATGTAAGTCTTGCATAAAT11.5151515151515151No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG11.5151515151515151No Hit
CTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGTAT11.5151515151515151No Hit
GATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTCTGTTC11.5151515151515151No Hit
GGACGGGACTGTGGTGTTCAGTTGTTCATTCCCAAAAGGGGGACGGGAGC11.5151515151515151No Hit
CTCTTTGGGTCGCCCTCTGATTAGTTAAACCCAGAAGCAATTTCTTATAC11.5151515151515151No Hit
TAGCAAGAGGACGTCTTTCCATTGATAATTAAGGTATGATTTGCTTCGCT11.5151515151515151No Hit
GAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAA11.5151515151515151No Hit
AGCTTCTTTAACTCAAATGATCTTCTCGTCTGAATTTGTGTGTCTCCCCT11.5151515151515151No Hit
TTGTTGATCCGTCCAGGCGCGCAGTCGCCCCTGGCCGCCATCCGGGCGCT11.5151515151515151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATTA50.0145.01
TTACCAC50.0145.05
ACCACAG50.0145.07
TATAAGT50.0145.0145
ATTACCA50.0145.04
CATTACC50.0145.03
CCACAGC50.0145.08
GCATTAC50.0145.02
TACCACA50.0145.06
CACAGCA50.0145.09
Produced by FastQC (version 0.11.5)