FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S4_R1_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG21.4285714285714286No Hit
GTCCAAACAAGTTAACTGATAGTTCCCATTGTCGTAAGTATTGTCAAGCT10.7142857142857143No Hit
AGGCAGTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTG10.7142857142857143No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA10.7142857142857143No Hit
ATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGTCATTGTTTATCATG10.7142857142857143No Hit
CCATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTT10.7142857142857143No Hit
GTGAATGTGAGAGGATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCC10.7142857142857143No Hit
CATCAGTATCATTTCTCAAATGAGACCTTCCTTTTATAATGAACCCATAC10.7142857142857143No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT10.7142857142857143No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT10.7142857142857143No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA10.7142857142857143No Hit
GAATTTGTGTGTCTCCCCTATGGCACCTATATGTGTATCTGTAGTCTTTG10.7142857142857143No Hit
CTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGACAG10.7142857142857143No Hit
CAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGA10.7142857142857143No Hit
CAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGA10.7142857142857143No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA10.7142857142857143No Hit
AACTTGTCTTGGGGAATATCTCAAACCTTTCAAATGATGACACTGAGCTC10.7142857142857143No Hit
CATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGT10.7142857142857143No Hit
GCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT10.7142857142857143No Hit
GTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGAACAGTCTGGGCT10.7142857142857143No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT10.7142857142857143No Hit
ATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATCATAAGTCTGG10.7142857142857143No Hit
GCCACATTTTCACCGAGTGCCCACTTCAATTGGCTTGTTCTCTTCATGTT10.7142857142857143No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG10.7142857142857143No Hit
GTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGAT10.7142857142857143No Hit
GACCTACCCAGCTATGGAGTGTCTGGAGTATATGAATCAGCTGACAAGAG10.7142857142857143No Hit
GTCCGAAAGAGGCGAAGAGACAATTGAAGAAAAATTTGAGATTACAGGAA10.7142857142857143No Hit
GGTTACTTGGGCTCTGGCTTGTCCGTTAAAAAACCCCAGAGAAAACAGTT10.7142857142857143No Hit
CTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATA10.7142857142857143No Hit
CCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGA10.7142857142857143No Hit
AGATCCCACAATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTC10.7142857142857143No Hit
GTCTTATATAGCACCCAGTGCTCCTTACATCACATGCCTTCCTTAGTGTC10.7142857142857143No Hit
ACTTAGCATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGATGCTG10.7142857142857143No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG10.7142857142857143No Hit
CGCTTGTCCTTTTGAAAGTGAACCCACCAAAACTGAAAGATGAGCTAATC10.7142857142857143No Hit
TCCCTAGTCTTGCCATTTTATTTGAGAACATTATGGGTGCCATGCTCAGG10.7142857142857143No Hit
GCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTT10.7142857142857143No Hit
GATAAGTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATT10.7142857142857143No Hit
CTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTT10.7142857142857143No Hit
ATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGA10.7142857142857143No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT10.7142857142857143No Hit
GTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAATAT10.7142857142857143No Hit
CATGGCGTCTCAAGGCACCAAACGATCATATGAACAAATGGAGACTGGTG10.7142857142857143No Hit
TGTTTCCGATTTGTTTTCAAAGAATTCCTTGGTCATGTCTTTCTCTTTGA10.7142857142857143No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10.7142857142857143No Hit
ACATGACCCCAAGCACGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAA10.7142857142857143No Hit
GAGTCAGTCGCTTGGTCAGCAAGTGCTTGTCATGATGGCATCAATTGGCT10.7142857142857143No Hit
GGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAATACTGCCC10.7142857142857143No Hit
GTATCACCTCTGGCCGTAACATGGTGGAATAGGAATGGCCCAACAACAAG10.7142857142857143No Hit
ATTCCACCTCTGGGTATATACTAAAAAGAACTGAATCTAGGGTCTTGAAG10.7142857142857143No Hit
CACTAGTACACTGTTGGCTTTTTTTTTTTTTTTTAAACCCATTACATTTG10.7142857142857143No Hit
ATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTC10.7142857142857143No Hit
AAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGA10.7142857142857143No Hit
GAGACAATTGAAGAAAAATTTGAGATTACAGGAACTATGCGCAAGCTTGC10.7142857142857143No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG10.7142857142857143No Hit
GACTCCTACTGGCCATTTCTTGTCTTATAGTGAAAAGCCTAGTTTTGATT10.7142857142857143No Hit
CCACTATATGATGCAATCAAATGCATCAAGACATTCTTTGGCTGGAAAGA10.7142857142857143No Hit
GTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCC10.7142857142857143No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACGGGAACAGGATACACCATG10.7142857142857143No Hit
GGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACT10.7142857142857143No Hit
ACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCTACTACCA10.7142857142857143No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG10.7142857142857143No Hit
GGTCATGTCTTTCTCTTTGACAGAAGACTCGGCCTCAATCATGCTCTCAA10.7142857142857143No Hit
TAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGAC10.7142857142857143No Hit
CCCTAAGTGCCTGGACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAAC10.7142857142857143No Hit
GAGAATGTAGGCTGCACACTGATCTGGCCTGCGGATGCCTTTTGTTGATT10.7142857142857143No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT10.7142857142857143No Hit
TTCTTGAAATAGGAGACATGCTCTTGAGGACTGCGATAGGCCAAGTGTCG10.7142857142857143No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG10.7142857142857143No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT10.7142857142857143No Hit
ATATAGTGGAATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT10.7142857142857143No Hit
CCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTGGG10.7142857142857143No Hit
GGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAA10.7142857142857143No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT10.7142857142857143No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT10.7142857142857143No Hit
ATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTAC10.7142857142857143No Hit
CATTTTCCTACCATGTCCGTGGTCTTCCTTGAATTGCCTTCCAGTATTAA10.7142857142857143No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT10.7142857142857143No Hit
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC10.7142857142857143No Hit
TTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGAT10.7142857142857143No Hit
AAACAAACGATGCTGGATCAGACCGAGTGATGGTATCACCTCTGGCCGTA10.7142857142857143No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG10.7142857142857143No Hit
GACATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAA10.7142857142857143No Hit
GGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGACATTAAAC10.7142857142857143No Hit
CAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCC10.7142857142857143No Hit
TCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGACTGT10.7142857142857143No Hit
TCCCAGTTCTGTACAAATTAGGAAATTTGTTCATAATTCCAGGCCCTGGA10.7142857142857143No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT10.7142857142857143No Hit
CTCCAAGATCAAGATGAAATGGGGCATGGAAATGAGGCGCTGCCTTCTTC10.7142857142857143No Hit
TACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGAT10.7142857142857143No Hit
CAAATTAATTTGGTTAAAATATGTGGTCCTCAGTAAAACTTTAGAACTTA10.7142857142857143No Hit
ATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGCTG10.7142857142857143No Hit
GCTTTCCACAAGAGGGGTCCAGATTGCTTCAAATGAGAATGTGGAAACCA10.7142857142857143No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG10.7142857142857143No Hit
AGTTTGATTATCGCTGCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAGC10.7142857142857143No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG10.7142857142857143No Hit
CTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGAC10.7142857142857143No Hit
GGACAAGCGGATCATCAGTCAAGAAAGAAGAAGAAGTGCTAACGGGCAAC10.7142857142857143No Hit
TGCCTGATGTCATTCAATCTTCTCAGCCACCCTGAGGAACAGGTATTACG10.7142857142857143No Hit
TTGTTCAACAAACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTAT10.7142857142857143No Hit
TTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTGGTCGGCAAGCTTGCGC10.7142857142857143No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT10.7142857142857143No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCAC10.7142857142857143No Hit
AAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGG10.7142857142857143No Hit
ATTCTACCCCACTTCTCCACCTGAGTGCTTTTCAAGCAGGGATGTCCGTC10.7142857142857143No Hit
AGAAAAAGGAGAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAG10.7142857142857143No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC10.7142857142857143No Hit
GTGGGAGATCAATGGCCCTGAGTCAGTGCTAGTCAACACTTATCAATGGA10.7142857142857143No Hit
ACACAGAGATTTCTTTCACAATCACTGGGGACAACACTAAGTGGAATGAA10.7142857142857143No Hit
TCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGA10.7142857142857143No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA10.7142857142857143No Hit
GCATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGC10.7142857142857143No Hit
TTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAG10.7142857142857143No Hit
GACCCCTATTTTATTTTAGAAATCTGGCCTTACTGACTTTAGCCCAGGAG10.7142857142857143No Hit
ATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTGCATC10.7142857142857143No Hit
TTCAGAGGACGGTGCACTTGGGAATGCAGGTACTGATGCTGAACTGTGGG10.7142857142857143No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT10.7142857142857143No Hit
CTTCAGCAGATTGAGAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAGAA10.7142857142857143No Hit
CAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAA10.7142857142857143No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA10.7142857142857143No Hit
ATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGAT10.7142857142857143No Hit
AAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTC10.7142857142857143No Hit
CCCACAATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGT10.7142857142857143No Hit
CCTGAAACTAACATTACATTGCATCTTAACTACCTGGAAATTGCAATAAA10.7142857142857143No Hit
GACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACAC10.7142857142857143No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT10.7142857142857143No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC10.7142857142857143No Hit
TATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATTGGAAA10.7142857142857143No Hit
GGAAAGAGCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCCCAAT10.7142857142857143No Hit
CTCCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTC10.7142857142857143No Hit
ACACTTGGCCTATCGCAGTCCTCAAGAGCATGTCTCCTATTTCAAGAACA10.7142857142857143No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT10.7142857142857143No Hit
GTTCAATTTTGGCGTTCACTTCTTTTGACATTTGGGAAAGCTTGCCCTCA10.7142857142857143No Hit
GTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAA10.7142857142857143No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC10.7142857142857143No Hit
GTTTTGGTGGGTTCACTTTCAAAAGGACAAGCGGATCATCAGTCAAGAAA10.7142857142857143No Hit
GTCTTCCCCGATAATCATCATCCATTAGCTCCCATTTTAAGCAGACTTCA10.7142857142857143No Hit
CTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAG10.7142857142857143No Hit
GAGGGCAAGCTTTCCCAAATGTCAAAAGAAGTGAACGCCAAAATTGAACC10.7142857142857143No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers