FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTTGCCATTTTGTTTGAGAACATTATGGGTGCCATGCTCAGGATGTT15.555555555555555No Hit
CTTCAAGACCAGCAAAATTTCAAGAAGGTAATCTTTCATCCCAGCACTTC15.555555555555555No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA15.555555555555555No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA15.555555555555555No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCGCGAGGAAATGTCACG15.555555555555555No Hit
CTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAA15.555555555555555No Hit
TATACAAATTCTCTATTCTTTTTTCCAGCTGGTTGAACTCTTTACCTACT15.555555555555555No Hit
AACATATGCCTCTGCATTCTGCTAGCTACTGTGTTGGTCCGCCCTAGTAG15.555555555555555No Hit
AAACTATTCTCTGTCGATTTCAATCTGTGCCGCATTTATTCAATTAACCA15.555555555555555No Hit
AGATTGCAGAACTCATTTTCCTGGCACGGTCAGCAATCATTCTGAGGGGA15.555555555555555No Hit
AACCAGTCTCGTGCCATTTCCTCGAGGGTCATCTCACAAAGCTAGCGCGA15.555555555555555No Hit
CCCAACGACATAGATCATGAACACATCAAGCCTAAACAAGAAAGGACACG15.555555555555555No Hit
GTAATACACAATATCAGGTGCAAGATCCCAAAGATATATCCTGCAAAGCC15.555555555555555No Hit
GCACTCGATCTAGTATGAGTCCCTATAGTTCTCATTGAATGTACAATCTG15.555555555555555No Hit
GACAAGAAATGGCCAGTAGGAGTCTATTGGATTCCTTTCGTCAGGCCGAA15.555555555555555No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTACCATGGGGCCAAGGAGGTGT15.555555555555555No Hit
TCTGAATTGTATCCGAAGAAATAAGACCATTCAGTACTCATGTCAAAGGA15.555555555555555No Hit
ACAGAAGACTCGGCCTCAATCATGCTCTCAATCTGCTGAAGAGACTGAAG15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)