FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTCCTTGGTCATGTCTTTCTCTTTGACAGAAGACTCGGCCTCAATCA16.25No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG16.25No Hit
ACTTTGGAAGTGTGTTTCATGTAGTCGGCTTTCCAGTTAATCGACGAACG16.25No Hit
ACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTT16.25No Hit
GACCAAAGATGCACAGAGAGGCAAGGTAAAAAGAAGGGCTATCGCAACAC16.25No Hit
ATGTAATGGAATCAATGAAAAAAGAGGAAATAGAGATAACAACCCACTTT16.25No Hit
CCCTTATACGGGAGCTCCTCCCTCCAGCCATGGAACCGGAAAAGGATCCC16.25No Hit
GTATTCATCTACTACCATTGTGCTGCCTATTATCCCAATCCGCGCCCTCT16.25No Hit
GTCTTATAGTGAAAAGCCTAGTTTTGATTCTTGCCCTGCTCTCTTCGTCA16.25No Hit
GGTTGTATCTTGATTGCGGTAAGTCAGTCGTCGATCGTAAAGTTGGAGTT16.25No Hit
ATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTC16.25No Hit
AGACTGAATAAGAGAGGCTATCTAATAAGCGCACTGACATTAAATACGAT16.25No Hit
CAGGTTGACTGTTTGGTAAGGGATTAGGCGTTCCCTTTACGAGACTAACG16.25No Hit
GTATAAATTAGGCATGTTTGGGGAGAGTGTGAGTTTCTTATTGGAGATGA16.25No Hit
GCAAACCCCAGGGATCATTAATCAGGCCCTCCTCGATTGCTTCATATAGA16.25No Hit
GTCTTCTCAACAGACATTATGAAAGAAAACTCTGTGACTTGTGACTTAGT16.25No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)