FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_F01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_F01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAGAGTGATCGCTCATGGGTATGGCTGCAGTGCATAATTATCGTTA13.7037037037037033No Hit
CTAAGGTCTGGCTAAAGGGCAGTTCGGGGGAAGTCAAAAGTAAAAGGTGA13.7037037037037033No Hit
TACTTTCCGAGAAATGAGAGTCACAAAGTGGCAATGTTATAGTTGAATAC13.7037037037037033No Hit
AAATGTATCAGATCATGACACAATAACAATGATAAAAACTTCAATAACTG13.7037037037037033No Hit
GTACTGGGGTTATCATGCCCTCGACTTTGGGCGGCGAATGATCGGAATCC13.7037037037037033No Hit
GTCATATATAAATTACAAAGGTACAAATTAATTAACAAGGTAAATAAAAA13.7037037037037033No Hit
CTCAAAAAACTGTCAAAGCTAACCTCTACCCTCTAAGTCCTGGATTACTC13.7037037037037033No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCAACGCCAG13.7037037037037033No Hit
GAACCCGGGCATGCACTAGCTAGCAGATCTTTTTACCTCTGACAAAAATT13.7037037037037033No Hit
CTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATTTAATGTCAG13.7037037037037033No Hit
TGTTGAGGATTCGAACTCAGCTCATTGACATCTAGCTGCGTGGAGGCATG13.7037037037037033No Hit
AGGCTAATGTGGTAATTCGGCAAGGAGACGTGTTGGAGGTAATGAATCGG13.7037037037037033No Hit
ATATGCTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGT13.7037037037037033No Hit
ATTCTCCTGGATTAAACTATAACAAGGCCACGAAGAGACTCACAGTTCTC13.7037037037037033No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCAAATCGCACGCAAAGAGGT13.7037037037037033No Hit
TTGATTGACAATGGGATTGCAATAAAACAGGATTGTTTACACAATCAATC13.7037037037037033No Hit
GTTAGGGGCGGGACTATGCATGCTGACTAATTGAGATGCGGATCCGCTGG13.7037037037037033No Hit
CAACTATACGTGAGGGCTTACAAACAGCCCACAGAGCTCCAATGCTAACC13.7037037037037033No Hit
ACGCTCACCGGCTCCACATTTATCAGCAATAACCCAGCCAGCCGCAAGGG13.7037037037037033No Hit
TTACATAGCTACCAAACTTGAGCGCTTAAAAACTGGCAAGAGAGCACCAA13.7037037037037033No Hit
GTGGGAGCCAGGATACTAACATCGGAATCGCAACTAACGATAACCAAAGA13.7037037037037033No Hit
CACTACGACCACTGGGGACCATATGTCCATAATCAACAAGGAAACATCAT13.7037037037037033No Hit
TAATACAGTAGATCAACTAAGTAATGATAATGATGATCATAGATCCATAA13.7037037037037033No Hit
GATATTAACCCTCGACACGGAAGCGCCCACAGCAGAATGGTTCCAGGACC13.7037037037037033No Hit
CATGATGGAGAGGAATAGGTCACAATGGGTGATCGCAGAGCCCCCGCCAG13.7037037037037033No Hit
GAGCTGAATTAAGCCATACCAAACGACGAGCGTGCCACCACGATGCCTGT13.7037037037037033No Hit
TTACAAAACCACTGTATTAGACTCTAATTGCCTTATTAACTAAATACACA13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)