FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGC22.631578947368421No Hit
GATTACAGCTGGAGGAAGAGTCAAGGTTCTGAGCAGGGGGTCGGGGGTGC11.3157894736842104No Hit
CTTTAAGGGTCAGCTCCACCAACCCACAGTGTGGGGCTGAAGCAGAACAG11.3157894736842104No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.3157894736842104No Hit
GAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAGATCGAAACTAACAA11.3157894736842104No Hit
ATGCTATTCTCATAAAGGCACCCAGGAGATTGATCCAGTTGCTAGTAAGC11.3157894736842104No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC11.3157894736842104No Hit
TCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTC11.3157894736842104No Hit
CATTAATTGCACTATTGAAGCTCCGATTTGAGATAATTGAAGGAAGAGAC11.3157894736842104No Hit
ACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAAGGAAATT11.3157894736842104No Hit
GCTTAATGATGTGACATCTCTTGTAGGCTTTGCAAAAAATGATAAATCCG11.3157894736842104No Hit
GGTGTTATGTGAGCCAAACCCGGATCGTCCAGAAGAGTGAGCGCTGAAAG11.3157894736842104No Hit
GTCCTAGCCTCCCTAATTGAGTGCATTTAAATATAAACTTAAGAGAAAGG11.3157894736842104No Hit
ATTGATGGCCATCCGAAATCTTTTGGTAGCTGTCTGGCTGACAGTAAGTA11.3157894736842104No Hit
ACTCAGGACAGAATGAAAGAAAAAGACAAAATCATACCTGAAATGAAGAC11.3157894736842104No Hit
ACTTTGTGCGACAATGCTTCAATACAAGGATCGTCGAGCTTGCGGAAATG11.3157894736842104No Hit
CAGTTAAATTTGCCATGCCATATGGGCAACTGTTCATGTTGCCCCAAAAT11.3157894736842104No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG11.3157894736842104No Hit
ATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAA11.3157894736842104No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11.3157894736842104No Hit
AAAATGACCTTGTACCTTCAACTGCCCAGATGGCTCTTCAATAGTGAATC11.3157894736842104No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC11.3157894736842104No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG11.3157894736842104No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT11.3157894736842104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.3157894736842104No Hit
ATTCAATAAGGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATG11.3157894736842104No Hit
GTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATTACATATA11.3157894736842104No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTTTT11.3157894736842104No Hit
CCAATGAAATGGCGACAGTTGAATAGATAGACAAAATCTGGTAAATCCTT11.3157894736842104No Hit
ATCTCAAGGAACAGGGAGGCCTGAGGACAGGGAGAGAGACGGGGAAACGG11.3157894736842104No Hit
CTGTTGCTCTTCTCCCAACCATTGTGAATTCTTCATACCCTTCATGTACT11.3157894736842104No Hit
CAGAATGAAGTGGATGATGGCAATGAGATAATGGACATCATTCCAGAGAG11.3157894736842104No Hit
TTGTAGGGTTTTTAGTTGGCCTAATTTCAATACTGTTGTATCTCAAGGAA11.3157894736842104No Hit
GTCAAGAAAGAAGAAGAAGTGCAAACGGGCAACCTCCAAACACTGAAAAT11.3157894736842104No Hit
CAGAAGGCTCTGTGGGGCTTGCTGGGCTGTGGATACCCCCAGGCCCCTCC11.3157894736842104No Hit
CTTCAATTGTTCATCACAGACTACATATACACATATAGGTGCCATAGGCT11.3157894736842104No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC11.3157894736842104No Hit
CCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTC11.3157894736842104No Hit
TGTGAGGAAGGAGTTGAACCAAGATTCATTAAGCAACACCCAGGGCTCAT11.3157894736842104No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC11.3157894736842104No Hit
ATATTCAATATGTATATAATAAAAGAACTGAGAGATCTAATGTCGCATTC11.3157894736842104No Hit
CATACTGTTTAAGTACTCCAACATCTTTGAAGTCATAAAAGTCTACTTTT11.3157894736842104No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG11.3157894736842104No Hit
CAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCA11.3157894736842104No Hit
GTTTGTTGCAGGTATTCGGATTTCCATTTCATCGACCAACGGGGTGAATC11.3157894736842104No Hit
CCAATAAGAACTGCTATGTAAAAATTTATCCAATATATTGCAACACATCT11.3157894736842104No Hit
GGAACAGGGAGGCCTGAGGACAGGGAGAGAGACGGGGAAACGGCCCAACA11.3157894736842104No Hit
AATGGGAGTAGATGAATACTCCATCACGCAGAGAGTGGTAGTGAGTATTG11.3157894736842104No Hit
GGAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGAT11.3157894736842104No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT11.3157894736842104No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG11.3157894736842104No Hit
GTGAGAACAAATTTAAACAACAAGATCAAGATGAAATGGGGCATTTAAAA11.3157894736842104No Hit
AGTAAGATCTGCCCAGGTACCTTTTATGCAACTGTGTCATTTGCAGTGAA11.3157894736842104No Hit
CATAAGCACCACATTCCCTTATAATGGAGATCATACATACATACATGGAA11.3157894736842104No Hit
AGGCAATGAAGGAATATGCGGAAGATCCGAAAATCGAAACTAACAAGTTT11.3157894736842104No Hit
GGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGCATGTCT11.3157894736842104No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG11.3157894736842104No Hit
AATCTATTCTTTGATCACCTTGAAATTAGTCTTCATTTCAGGTATGATTT11.3157894736842104No Hit
ACTCCGAGCAGCTGGAAGCAGCCACTGGCCCAGTCTCTCCACGCCACAGC11.3157894736842104No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG11.3157894736842104No Hit
AGTATGGATAGCAAATAGTAGCATTGACACAACTACTTCAGTGCATGTGT11.3157894736842104No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCTATCAAAAGATCGTC11.3157894736842104No Hit
ACTGACCCGAGACAGGAGCCACACAAATGGGAAAAATACTGTGTTCTTGA11.3157894736842104No Hit
TTTTAGGACATTATGGATATCAAATAGTAGAATTGCCACACCTACTTCAG11.3157894736842104No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11.3157894736842104No Hit
GCTCTTCGCCCAACCATTGTGAATTCTTCATACCCTTCATGTACTCTTAT11.3157894736842104No Hit
CAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAG11.3157894736842104No Hit
TGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACA11.3157894736842104No Hit
GTATTCAACAGTCTATATACGTCTCCACAACTTGAGGGGTTTTCGGCTGA11.3157894736842104No Hit
CAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGA11.3157894736842104No Hit
CATATAGGTGACTTATTGTAGACACAAAAATACAGACGAGAAGATCATTT11.3157894736842104No Hit
GAATGAAAGAAAAAGACAAAATCATACCTGAAATGAAGACTAATTTCAAG11.3157894736842104No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT11.3157894736842104No Hit
CAGTCAACTATACTTCGGTTAAAAAAAAGAATTATTAAAAAGAGGGACAG11.3157894736842104No Hit
CATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATGTGGTGAACT11.3157894736842104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG50.0145.05
TTATACT50.0145.04
CTGGAGA50.0145.09
CTTATAC50.0145.03
TACTGGA50.0145.07
CCTTATA50.0145.02
GGAGGCC50.0145.0145
ATACTGG50.0145.06
CCCTTAT50.0145.01
ACTGGAG50.0145.08
Produced by FastQC (version 0.11.5)