FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S4_R1_E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT17.6923076923076925No Hit
CATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGATA17.6923076923076925No Hit
GCGACAGGACATTGAAAATGAAGAGAATCTAAACAGTAAATACAACATAC17.6923076923076925No Hit
GTCTGATTTGAAGCAATCTGGCCCCCGCATGTGGAAAGCATTCCTCGTGG17.6923076923076925No Hit
CCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGC17.6923076923076925No Hit
TCTCAGAGAACTATTCTTTCTTGATCCGTCCAGACTCGAAGTCGACACTG17.6923076923076925No Hit
AAAGTGTGATTAGTGAGTATCTCTCGATTGAGGGACTGCGACATTAGATA17.6923076923076925No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG17.6923076923076925No Hit
CTAATTTACATTGAATCTAACATCCATAACATGTAGAAACTGAAGACTCA17.6923076923076925No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT17.6923076923076925No Hit
GTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAAATGAA17.6923076923076925No Hit
GATTGAACATATCGCAAGCATGAGGAGGAACTAAATAACAGCAGAAGTGT17.6923076923076925No Hit
CTACAGATACACATAAAGGTGACAAAGTGGAGACACACAAATTCAGACGA17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)