FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_E09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_E09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22.272727272727273No Hit
TGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAA11.1363636363636365No Hit
GGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAG11.1363636363636365No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC11.1363636363636365No Hit
ATAATATAAGATGACTTAGTTTATCAATATTCTTCTTTTTTGATAAAATG11.1363636363636365No Hit
CCCTTCATGTACGCTTTTTTTCAGTGTTTGTAGGTTGCCCGTTCGCACTT11.1363636363636365No Hit
GAATAGGGCGATGATCCGAAAATCGAAACTAACAAGTTTGCTGCACTATG11.1363636363636365No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT11.1363636363636365No Hit
GGACACAGTAAACAGAACACACCAATACTCAGAAAATGGAAAGAGGACGA11.1363636363636365No Hit
TTATGGGTGCCAACATCAGGATGAATCTGAACCACACGGGTAGAATACTG11.1363636363636365No Hit
GATTAACTGCACTATCCATGTAGTTCGGGTCCCCATTCCCATTTAGTGCA11.1363636363636365No Hit
CAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTA11.1363636363636365No Hit
CATGAAGGGTATGAAGAATTCACAATTGTTGGGAGAAGAGCAACAGCTAT11.1363636363636365No Hit
GCGCAAGCTCATCCCCCAATTTCTAGTACAGTGTCATTACGAATACACTG11.1363636363636365No Hit
ATATCAGGTTAAATCTCACAATGATATTTTATGCAAGGACGAGAGTATAC11.1363636363636365No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG11.1363636363636365No Hit
GGGTGGGATTCTTCAAGGAAAGCCATAGCCTCTAGAACACAGTCTGTTTG11.1363636363636365No Hit
CAGAATGTTGCTGTCTAACAATAACGCAAAGGCTATGGAACAGAAGTCAG11.1363636363636365No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTCAGAGATCTAATGTCGCA11.1363636363636365No Hit
GAGTTCTTCAAGGGTGGCACAGCTCTGCATGAGCGCCGAGAACGCGTCTT11.1363636363636365No Hit
GCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAG11.1363636363636365No Hit
CAACACCCCCATGGAACAGGTAACGTACCCAACATCCACCCCGTAAAACG11.1363636363636365No Hit
TTAGCCTTCTCTCCTTTTGCAAGATTGATCAGTTCATTGATGCTTAATGC11.1363636363636365No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT11.1363636363636365No Hit
AATATATTCATTATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA11.1363636363636365No Hit
TTCTTGCCCCCACTTCATTTGGGAAAACAACTTCCATAATCACATCCTGT11.1363636363636365No Hit
AACGTACGTTCTTTCTATCATCCCGTCAGGCCCCCTCAAACCCGAGCTCG11.1363636363636365No Hit
GGTTACGACAACTCTCTCAGTGCAGGTCGATACATCAACGCACAATTTGC11.1363636363636365No Hit
CCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGGCTCTGGC11.1363636363636365No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTC11.1363636363636365No Hit
CATATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGC11.1363636363636365No Hit
GCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCT11.1363636363636365No Hit
ATCCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCAT11.1363636363636365No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGCTGCA11.1363636363636365No Hit
TCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGA11.1363636363636365No Hit
GCCCGAAATGACCCCAAGCACGGAGATGTCGCTGAGAGGGATAAGAGTCA11.1363636363636365No Hit
TTTTAGATAATAGATAAACAAACTTTATGCAACAACATCATGTCTAAATT11.1363636363636365No Hit
AGTAGAAACAAGGCATTTTTTAATGAAGTAAAAGTTAAATTCATTATTTT11.1363636363636365No Hit
GTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGAT11.1363636363636365No Hit
GTGTACGACAAACCCAGAGACTGGTGCACCCCTGCTCACCCCGCTTGTTG11.1363636363636365No Hit
GTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGA11.1363636363636365No Hit
CTGTGGACAATAAATCCATAATCAAAAAGTAGACATAAGGAAGGCAAGAC11.1363636363636365No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT11.1363636363636365No Hit
CCGATAGTCATCATCCATTAGCTCCCATTTTAAGCAGACTTCAGGAATGT11.1363636363636365No Hit
GAGGAACAGCATGTGATTATGCAAGATGTATACCCAAATGAAGTGGGGGC11.1363636363636365No Hit
TTGCCGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGCATG11.1363636363636365No Hit
CCCTAGCCCAATTAGCACATTAGCCTTCTCTCCAAATGCAACATATCTCA11.1363636363636365No Hit
CCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTC11.1363636363636365No Hit
GAGTAAGGAGGGTGGACATCCTTAGACAGAATCCAACTGAGGACCACGAC11.1363636363636365No Hit
AGACTGAATAATAGAGGCTATCTAATAAGATCACTGACATTAAATACTGT11.1363636363636365No Hit
CAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTTC11.1363636363636365No Hit
AATCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAG11.1363636363636365No Hit
AGTTTCATAAGAGGTAAGAAAGTGATTCCGAGAGGAGGGCTTTCCACAGG11.1363636363636365No Hit
GGCACTCCTCGATTGCATCATATAGCCCCCCAAGATCGAAGGTTACAGGT11.1363636363636365No Hit
GACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGG11.1363636363636365No Hit
ATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATGTGGTGAACTTTGT11.1363636363636365No Hit
TTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGTCATTGTTTATCATGT11.1363636363636365No Hit
AATCCGGGCCCTAGACACCATGGCCTCCCCCATGCTAGAAATTCCAACCG11.1363636363636365No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC11.1363636363636365No Hit
TCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGAC11.1363636363636365No Hit
TTTCTCGAATAGATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAA11.1363636363636365No Hit
CCTGCACACTTTCCCAATAGAGACAACCTCCACTCCCCTGGGTCACTCTC11.1363636363636365No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA11.1363636363636365No Hit
TCCCTGAGGCAACCAGAAGCCGGTACGGTGGCGTCGTACGGTCACTGTTC11.1363636363636365No Hit
AATACATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA11.1363636363636365No Hit
AGTTTGGTCCTCCAACTGATAGTTATGGGCCTACCTTTGATTGGGTGTGG11.1363636363636365No Hit
TCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATTAGCCT11.1363636363636365No Hit
ATTATGTATTCATTAGCCCTGCAGTGGTACACTTCGGCTGTAAAATAGTT11.1363636363636365No Hit
GGGTATGACTTACAGTATTAAACTATCATTATAAAATTTATGTATACGCT11.1363636363636365No Hit
AAGTGTGAGAAGGCTCTGCAGGAATGGAAATAACAAGGGGACCGAACAGT11.1363636363636365No Hit
TTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGCAGAAATGCT11.1363636363636365No Hit
CCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTGGAATGGA11.1363636363636365No Hit
AGGTAATTGGGATTTATGCCTATCTCATGTTGTTTCACTATGTTAGTCTC11.1363636363636365No Hit
GTTCTAGAGGCTATGGCTTACCTTGAAGAATCCCACACAGGAATATTTCA11.1363636363636365No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG11.1363636363636365No Hit
CCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCAC11.1363636363636365No Hit
ACTCTTCTTTCTTGATCCGTCCCCAAGCTAATGCGCCCCTGGAAAAAAAA11.1363636363636365No Hit
TCCCTGAACAATGAGAAGCAAATTTCTAGATTCAGCAGAAAAACAATAAA11.1363636363636365No Hit
CTTAATGCTGGCAGTCTTGTCTTCTTTCTCCAAAATGAGAAATGCTCACA11.1363636363636365No Hit
CATGTCTCCTATATCAAGAACACAGGATTTTTCCCATTTTTGTGGCTCCA11.1363636363636365No Hit
GTATTCGGATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAG11.1363636363636365No Hit
TTGATGCTTAATGCTGGGCCATATCGCTTGTCTTCTTTGCCCAAAATGCG11.1363636363636365No Hit
CCTTTCGCATATCCGCAATCTATATGAACACAATGGAATGGGTGAACACC11.1363636363636365No Hit
CCCACAACTACTTCAGTGCATGTGTGAGGATGGAGTTGAACCAAGATGCA11.1363636363636365No Hit
GGCGTGGGGTACTGCCTGCGGGGGTTGGAAGGCTTGCTGATCGGTTGTTG11.1363636363636365No Hit
GAATAAAAGAACTGAGAGATCTAATGTAGCAGTCCCGAACTCGCGAGATA11.1363636363636365No Hit
GCGGTAAAAGGTCCTGCGGATGGAGGCTCTTCACTTAAGTAGTGAAGACC11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTCT50.0145.04
TAGCCTT50.0145.02
CTCTCCT50.0145.09
ACATCAT50.0145.0145
TTCTCTC50.0145.07
CCTTCTC50.0145.05
TCTCTCC50.0145.08
TTAGCCT50.0145.01
CTTCTCT50.0145.06
AGCCTTC50.0145.03
Produced by FastQC (version 0.11.5)