FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_D09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_D09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG21.550387596899225No Hit
ATTTCACATAGAACCAAATGACCTTCAAGTCGAAATTGGTCAGGTTAGAT10.7751937984496124No Hit
GATCCCAATGATAAGCAAATGTAGGACCAAAGAAGGAAGACGGAAAACAA10.7751937984496124No Hit
CGCCTCGATTGCTTCATATAGCCCCCCACGCTCGAAGGGTCCCGGTTCCA10.7751937984496124No Hit
GAGGAAGGCTCTATTGGGAAAGTGAGGCCGAGTCTTCTGTCAAAGAGAAA10.7751937984496124No Hit
CATGTACCCTGGGGCAAATAATACATTATATGTTAATTTAAAAAAAAAAA10.7751937984496124No Hit
GACTAAATAAGTGTATGTCTCATACCAAAAGTGATTAAATATTATTGATT10.7751937984496124No Hit
TGCCACAACTACTTCAGTGCATGGGGGAGGAAGGAGGTGCACCAAGAGGC10.7751937984496124No Hit
ATACTGGAGAGCCTCCCTACAGCCTGGCAACAGCTGCAGGCGACACCAAG10.7751937984496124No Hit
GAACTAAATGCCCCGAGACTGGAGCCACACAAATGGGAAAAAGACTGTGT10.7751937984496124No Hit
GGGTAGTAAACACAACATAACAATATAATTCTACCAATCCATGAGCATCC10.7751937984496124No Hit
ATGCTGTTCTCCAGGGCCGCGGTTTTGGTGATGATTTCCCTAGTGCTTTC10.7751937984496124No Hit
GGGCTGGCCTGTGGAGCCCCCAGATAAGGGATCTACACCTTTCCACTTCC10.7751937984496124No Hit
GTGTTTGCATATCCTGAACTGCCGGGTGTTGTCCCAATTTCAAATATTCT10.7751937984496124No Hit
CTTCAGCAGATAAAAGCCTGACCACCTTGCTAGAGTACCCCAGAGCATTA10.7751937984496124No Hit
AGCACGGAGATGTCGCTGAGAGGGAGAAGAGTCAGCAAAATGGGAGGAGA10.7751937984496124No Hit
GTATATACATCCATATATACTTACACAGTTTGAGCATTCCCAGGCCACTA10.7751937984496124No Hit
GTTCAGTAGTGGTAAAAGTGGCATCGGGAGAAGGGAGAAGGAACCAGGAT10.7751937984496124No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC10.7751937984496124No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT10.7751937984496124No Hit
AGCAAGTTTGCTGCAAGATGCGCACATTTGGAAGTGTGTTTCTTGGATTC10.7751937984496124No Hit
CTTGTGCTAGGATGGCCACAGCTGGTGAGAGAGGCAGGTTAACACGAAGG10.7751937984496124No Hit
GTTCTAAGGGTCTATACCTAGGAAGAGCAGTCGATGCTAGACTATGTGAT10.7751937984496124No Hit
TCCATTATATTTGGAATTAACGTCTACGTTCTCTCCGGGGGAATGACTGT10.7751937984496124No Hit
ACCCCTGAGGTTACATAGGACCGACTTCACATCTTGGAAGAGATCTATGA10.7751937984496124No Hit
ATACCTGACGGCTCATACAGCCTTCAATTTCAGGCCATTCCCTAAGCCAT10.7751937984496124No Hit
GCAAATAGTAGCATTGCCACAACTAATACAGTGGATGTGTGAGGAAGGAG10.7751937984496124No Hit
GAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTTTCAAAGAAA10.7751937984496124No Hit
GGGGGGGGGCGGCCGTGGCGGCGGTCGGGGCGGTGGGGGCGGGGGCCGCG10.7751937984496124No Hit
CCCCAGAGTGCGTGCTCGTGTTCTTTGTTCCTCTGCCAGGGGTTCTGGGG10.7751937984496124No Hit
AATGAAGTCCTATATTAATAAGACAGGCACATGTGAATTCACAAGCTTTT10.7751937984496124No Hit
ATACTAAATTGTCATATTAAATATCTCGAGTGGGGATTTTTAAGGGCTTT10.7751937984496124No Hit
GTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATG10.7751937984496124No Hit
GGATGCTCTGAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGGGAA10.7751937984496124No Hit
TATTTGTTCATCCAGTTCATGAGCATGGAATGTCTTTCCATTTCTTCGTG10.7751937984496124No Hit
ATGCTGCACCGTGTGACATCAACTTCCGCTGGATCTGTGAGAAGGCAGCC10.7751937984496124No Hit
ACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATT10.7751937984496124No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT10.7751937984496124No Hit
GACTTTGAGATGGCTGTTAGAATTCTCATGGAGGTGTTGGGCAGACGGGT10.7751937984496124No Hit
CCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA10.7751937984496124No Hit
CCACTGTTTCGGCTAGAGATGGAGCAGAGCTGCTCCTGCTAAGCTGAAGA10.7751937984496124No Hit
CGTGAGCAGCCAGGATCGTGTAGGTTGTGAGATATAGAAGAGCGCTTAGG10.7751937984496124No Hit
TCAGAATTCTTTTGGTCTCTTTCTGGATGTCAGAAAGTATGATACAGTCC10.7751937984496124No Hit
CTGCACCTCTTTTCCAACCCTCGTCATGTCTCCTTGTACTCTCAACACCA10.7751937984496124No Hit
GCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATCATAAGT10.7751937984496124No Hit
ATCATAGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTAGTGTT10.7751937984496124No Hit
AGCAGGTACTGATCCAAAATGGAAGCCTTTGTGCGACAATGCTTCAATCC10.7751937984496124No Hit
ATTGCGCAGAGTCTGGGAGTTTGGACATCATTAAGATGCTGCTTATGTAT10.7751937984496124No Hit
ATGATGATCAGAGCCAAAGCTTAATGACTGAACCACCCAAGCGCCCCTCT10.7751937984496124No Hit
GACCTCTCACCCAGACAACTACCTACAGTCTCCAAACTTTGTGCACACAC10.7751937984496124No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG10.7751937984496124No Hit
GTGAGAAAGATGATGCCTAATTCACAAGACACAGAGATTTCTTTCACAAT10.7751937984496124No Hit
CCTTGAAACAGGCAGGCTATAACGGAGGCGTGATATGGCCAGACAAGGGA10.7751937984496124No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT10.7751937984496124No Hit
CACTCAGATTAATTGGTCAGAGCTTGATATATGGGTTCAGATGTCAAAGA10.7751937984496124No Hit
GCAGATAACTGGGCAGCGGGGGCACGGTGCAGTCCATACGTACACTGGGG10.7751937984496124No Hit
ATTAGTATCAGATGGAGGCCAATACTTATAGAATATGCGGAATCTTCACA10.7751937984496124No Hit
GCCTAGGAACGTCGGATCATGACCTGAGATGAATGAAGACTCTTAACTCA10.7751937984496124No Hit
TAGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGACATTAAACAGA10.7751937984496124No Hit
ACAAAGCACACGGAACAATAATGAGAAAGTTTGGAAGATTGAAAAAAATA10.7751937984496124No Hit
CTAATTGAGGTGTTGGTCAGACGGGTTGATACTGAAGTCTGGGATTCATA10.7751937984496124No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC10.7751937984496124No Hit
GTGTTTCCTACCTCTTAGCTGGGGGTGAGTTGCCCGTAGTGTTAGTATGG10.7751937984496124No Hit
GTAAAGGAGCATCACTCTGGCATTGTCTCCATTGGGTGCACTACAAACAA10.7751937984496124No Hit
ACTTACAGAGCATCCAAATTCTAAGCACATGGAGTACCAACGCAGCAATA10.7751937984496124No Hit
AAGAAGTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTT10.7751937984496124No Hit
GGCCGCTCCATGGAATGTAGAGCATTAACATCATCATGCCACTTAGGATT10.7751937984496124No Hit
TATCCACAGAATGTGGATTACACATGGGATCAGAGGTCTAAGCGGGCACA10.7751937984496124No Hit
GCTTGAAGGCGTGAAACCCCAGCCCTTCCCCCAGTATCACGCACCTTGTC10.7751937984496124No Hit
CTGTGGCATTTGGCAAGGCATTCTACCATCTGGAAGCCGGTACAGGTACC10.7751937984496124No Hit
ATCTTATTCAGTCTTTGTTTTTTCTACGCTATTGTTGTTTGCGTGTGCAT10.7751937984496124No Hit
AGGAGATAGGGCGAAGGGAGGGAGAGACTCTGAGGCAGAGGACTCTGTTG10.7751937984496124No Hit
ACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATC10.7751937984496124No Hit
GTACACAGAGCACATTCAAATATAATTCAAAAGCCAAACAGAAGGGCTTG10.7751937984496124No Hit
GGGCAGACATTACCACAGCATTGTTTACAGAATAAAACTCGTTATGTCTG10.7751937984496124No Hit
GATACTAATAGCCCTACCTTTGATTGGGCTTGATCCCACAGCTTCTTTAA10.7751937984496124No Hit
CCCCAGTACCTGCTCCAGAGCTCTCCTGGCTGCCCCCCGAGTCATCCGGA10.7751937984496124No Hit
CTTCGACGCAGCGATAAAACGTCCTTAACATGAAGAGTAACCAACATTGT10.7751937984496124No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA10.7751937984496124No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGC10.7751937984496124No Hit
AATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGC10.7751937984496124No Hit
TGAGGACCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGA10.7751937984496124No Hit
GGTAAACATCATTATATTTCTCTGTCTATATGAGCTTCAGGACTCTAGGC10.7751937984496124No Hit
CTTCTTGGTGATGGGGCGGGGGCTCGGCGGGCGGGGTTGAGGGTGGGCTT10.7751937984496124No Hit
ATTGAAAAGAGGGAGAATTTCCGTATTATTATCTTGAAATTGATTATTAG10.7751937984496124No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA10.7751937984496124No Hit
AAAAAAAGCTTGTGATATCAAATGTCACTGTCTTATTTATATATGACTTC10.7751937984496124No Hit
CACCCACACACACCCACACACACACAGTCAGAAAACTGAAAGAAGATAAA10.7751937984496124No Hit
CTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTG10.7751937984496124No Hit
CCTATAAACAAGTTTTTTTAAAAGCATATGACGTTTTTCTGATTCCTAAA10.7751937984496124No Hit
AGTATAGCAAGTGTCACGTAAGTGTTAGCTCTAGGCGTCGTCTTACCCTT10.7751937984496124No Hit
CCCACGTTTCCTCCCGCAACACCAAGACCAGCCTGCAGGGAAGCTGGGGG10.7751937984496124No Hit
GCCTAAGATTGTTTTTTTTTTTTTAAATTTTTTATTTGACATGCTAAATA10.7751937984496124No Hit
GATAACAACTAGGCAGAAAGGCAGGCAGAGAGAGAGGGGGAACCACGCTC10.7751937984496124No Hit
ATATTATGTGTAGAGGAAGAATTAGATTCGCAGTTAGGAGCTCGGAACTC10.7751937984496124No Hit
GTTAACAACTGTGCCCACCAGGCAGTAAATGGGAACCATCTTCCGAACAC10.7751937984496124No Hit
CTGAGGTCAGGCGGCGCAGGTGGGCCAGGATTAGCGAGTGGCTGACCATG10.7751937984496124No Hit
CGACACGCACAGACACTGGTGCACCCCAGCTCCACCCGATTTATGGACCA10.7751937984496124No Hit
TCTTTGTCCTTGGGATCTTCTCTTCATTTTCAATGTCCTGTAGCTCTGCT10.7751937984496124No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGTGGGGGTGGGGGGCGG10.7751937984496124No Hit
AATGTCACAATGTTACGAGATTATGTACACCGCAGTGGAACAATTGCACG10.7751937984496124No Hit
GAGCTGGTCCGTGAAGATGCTATAAAACAGGCCCCTGCAGGCCATGAGGA10.7751937984496124No Hit
GTGTTATGTTAGCCAGGATTTTTTTTTTTCGTTTCACCCAAGCAGTGGGC10.7751937984496124No Hit
GTGATATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATT10.7751937984496124No Hit
CATTGAGCAAGGCGGTATTTATGTACACTCCCTTCATTATGTATTCAGTA10.7751937984496124No Hit
GTGTGGATGGCCGTCAGTCCTGCGCTGCCAATTCCTGTGTCGTATTGACA10.7751937984496124No Hit
GCTCTTGATCTCGGGGTCATGAGTTGGAGCCCCGTGTTGGATGTAGAGGT10.7751937984496124No Hit
GAGCTGGGGACAACTGTGGTGAATCAGGGTTTTGTATGTTCCGTGGTGGG10.7751937984496124No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT10.7751937984496124No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGCCATTAAATACGATG10.7751937984496124No Hit
GTGTTGAGGTAGAGCCTGTGGCTAAAACTGTGAACCAGCCAACTGTCCCC10.7751937984496124No Hit
AGTAGGAACCTCGTCGAGATGTTTAAATTCGGGACAAAAAGTTGGGCCCC10.7751937984496124No Hit
CTTGAAGACTCCACGTTCCCCAAGGCGGAACCTTTATCAAGCGCTATATT10.7751937984496124No Hit
GAGGGTATGTGCAGGAGGCAACTGGAGAGGTGGGTTTGAATTTAAAAGGA10.7751937984496124No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT10.7751937984496124No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT10.7751937984496124No Hit
CTGTCAGTACGACACTAAGTATCCTACATACCCGTTACTTTTTGAATAGA10.7751937984496124No Hit
AAATTAAAATAATTACTTCCGACACAGAAGAAAGGAAGAGAGCAGTAAAT10.7751937984496124No Hit
GTCCACCAGTTCTCACTCGGAGGCCCGTGTCTCCTCTCTGTTTTATCCTG10.7751937984496124No Hit
CCAGGGTGAGGAGGCGGTCTCGCCTGGTCCTCTAGGGGCGGGGGTGGGCG10.7751937984496124No Hit
TCTTTGTACTGAAGAAGGGCAACGACAACTGGGCGACGGTCATATTCAAC10.7751937984496124No Hit
TGCTAGAGTCCCGTTGTAGTGTCATTTACACCCCAACGTCCACCTCCCCA10.7751937984496124No Hit
AAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATT10.7751937984496124No Hit
GACTGGAGCCACACAAATGGGAAAAATACTGTGTTCTTGAAATAGGAGCC10.7751937984496124No Hit
TCCCCTTGCCCAATTAGCACGTTAGCCTTCTCTCCTTTTGCAAGATTGCT10.7751937984496124No Hit
ATCTCCAACTGACTAAAAGTTGGTATCAGAATGAATTGTGGGCAACGAAT10.7751937984496124No Hit
CCTTGGGGGAGTAGATGCTGCTGTGGCACACACCGGGAGAGATCTCCATG10.7751937984496124No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10.7751937984496124No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers