FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_C09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_C09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATATGTGTATCTGGAGTCTTGGATGCACAAGTGAAGAGCCCGCGGG11.4492753623188406No Hit
GTATACACCATTGAAAAAGTAAACAGACAACAACAATACTCAGAAAAGGG11.4492753623188406No Hit
CCTGGAGCTCTTCTGTCGTCTCTTTTGTTATTGCCAGCTGGGACTCTGAT11.4492753623188406No Hit
GGATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGATATA11.4492753623188406No Hit
GTCCTAGGATGCTGATGGGCATCTTCAGCATGATAAGAACGGTCAAGGCA11.4492753623188406No Hit
CATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTT11.4492753623188406No Hit
TCTCTTGTCTTCTTTGCCCATAATGAGAAATCCTCTCAGGCCAGCAGACT11.4492753623188406No Hit
CTTTCCCAATAGAGCCGTCCTCCCCTCCCCTGGGTGACTCTCCGATTGGC11.4492753623188406No Hit
GTCATACTCATCTGCATTGTCTCCGAAGAAATAAGACCCATCATTACTCA11.4492753623188406No Hit
AATAGGAATGGCCCAACAACAGGATGCAATTTTCCTCATTGACTGTGAAT11.4492753623188406No Hit
CTCCACCATATAGCTTCCTTGCATATGTCTACGGCTTGGAACTCAGTTGG11.4492753623188406No Hit
GTGGAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCTGATAGTAATA11.4492753623188406No Hit
GAACTTTGTCAATAGTTAAAACCAGCGAATGCACCCACTGAACAAAATCT11.4492753623188406No Hit
TATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGA11.4492753623188406No Hit
CCGGAAAACGCAATGAAAGAATATGGGGAATATCCGAAAATCGAAAATAA11.4492753623188406No Hit
GAGTTAAAGAAGCTGTGTGATCAAACCCAATCAAAGGTAGGGCTATTAGT11.4492753623188406No Hit
TTATAACCGAGGTCGAAACGTACGTTAGTTCTATAATCACGTCAGGACCC11.4492753623188406No Hit
CTCCAACATTTACCCTATCCCAACACATCCAAATTTGTTTGACAACAATA11.4492753623188406No Hit
ATACTGATGTGGTGAACTTTGAAAGTATGGAGTTCTCCCTCACTGACCCG11.4492753623188406No Hit
CATTGTCGATTTATTCAATTCCCCAGTTCTGGAAAAGCACTTTTGAATCT11.4492753623188406No Hit
ATTTAATACTGGAGAATATACTATTAACAGTATCCTCAACAGTGATACTC11.4492753623188406No Hit
GGCCATGGTATTCTAACAGTAGGATTGCATGATCAAGGCAGTTAGGGGCG11.4492753623188406No Hit
TTCCAAGCGGGACCATCATATGAATGGTTCCCTTATGACAAACACATCCC11.4492753623188406No Hit
TATTTTCACCTAGTGCCCACTTCAATTGGCTTGTTCTCCTCATGTTCTTT11.4492753623188406No Hit
TCAGTCAAGAAAGAAGAAGAAGTGCTAACGGGTAACCTCCAAACACTGAA11.4492753623188406No Hit
ATTTTATACAATAGCAACATAGATGCTCATACAGGCACACAGGATATTTA11.4492753623188406No Hit
AACTAACTCAAGGTCGCCAGACTTATGATTGGACATTAAACAGAAATCAA11.4492753623188406No Hit
GTTCCAGTTTGTACATAATTGCCTGACCAATGAGAAGCAAGTTTCTAGAT11.4492753623188406No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT11.4492753623188406No Hit
CGAACTATAGTCAACAGAAGAAGTCAGTGAAACGAAATCAACTGACAAGT11.4492753623188406No Hit
AGCTGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCATGTTCAACA11.4492753623188406No Hit
ATGATGTCCGAAAGCAAAGCAGCTGCCTCAGAGCTCGTAAACACTTATCA11.4492753623188406No Hit
CCTTAAACAGTATGAAAGTGATGAGCCATAGCCAAGATCTCTAGCAATCT11.4492753623188406No Hit
TTGTTATTTCTAACCAATAGTTCTTTTCGTGAACAAATTATTTGACATGT11.4492753623188406No Hit
TGTTTCATGTATTCGGATTTCCATTTCATCGACGAACGGGGTGATTCAAT11.4492753623188406No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA11.4492753623188406No Hit
TCTTTGTACTTGGGATCTTCTCTTCATTTTCAATGTCCTGTAGCTCTGCT11.4492753623188406No Hit
GTCCAAGGTCATTGTTAATCATGTTGTTCTTTATCACTGTTACTAAAAAA11.4492753623188406No Hit
ATCACTGTGATGTCTCGTAGATGGCTAAGATTAATAAAAATGTGAGTAGA11.4492753623188406No Hit
GCCCTAGACACCATGGCCGCCACCATGCTAGAAATTCCCACCGGTCTCCG11.4492753623188406No Hit
AATAAGAGCACTGCCATTAAATACGATGACCAAAGATGCAGAGAGAGGCA11.4492753623188406No Hit
GCTTTGTACAAAATGACCTAAATGGGAATTGGGACCATAACAACATTTAT11.4492753623188406No Hit
CTTGCAATATGTTCAATCGGGGCAACATCTTCTCCTAATTAATAAAGTAC11.4492753623188406No Hit
ATCAGTAACTGGTAAATTGTGAAAATTCAATGGTCACAAGATCCCACAAT11.4492753623188406No Hit
ACCATCGCCTCCACCAAGCAAGAAATTCCAACCGGTCTCCTATATGAACA11.4492753623188406No Hit
GCGATAGCCCTTCTTTTTAACTTGCATCTCTCTGCATCTTTGGTCATCGT11.4492753623188406No Hit
TGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTT11.4492753623188406No Hit
ATTGTGAATTCTTCATACCCTTCATGTACTATTATTTTCAGTGTTTGGAG11.4492753623188406No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG11.4492753623188406No Hit
CTTCCATATAAACACTTCCTGTTCCGCAGGCTACTGGGAGAAACATTGTT11.4492753623188406No Hit
ACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATT11.4492753623188406No Hit
ATTTCAGTCTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCG11.4492753623188406No Hit
GAGCCGAAGTGTCAGCAGACCCATTAGCATCTCTCTTGGAAATGTGCCAC11.4492753623188406No Hit
GGAGAATTGCATATTACCAGTATACTCAACCATGATACGCTAACATTCAC11.4492753623188406No Hit
AATTAGCACAGTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA11.4492753623188406No Hit
CACGGAGGGAAGTCCACATATATTACCTAGAGAAAGCCAACAAAATAAAA11.4492753623188406No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG11.4492753623188406No Hit
CAATTGCTGAGGCAATAATTGTCGCCATGGTATTCTCACAGGAGGATTGC11.4492753623188406No Hit
GGGGGGCGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.4492753623188406No Hit
GAACTTGATGAAATAGGAGAAGATGTTGCCCCGATTGAACATATCGCAAG11.4492753623188406No Hit
CTTCCAAGCCATGAGGAAATTGGGATTTATGACTTTCTCTTGTCGTTTGA11.4492753623188406No Hit
GTATCAACTCTCCTCCTTATTTTAAGTTGATTTCTGAAGTGGAAAGGGCC11.4492753623188406No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC11.4492753623188406No Hit
CGTCTCCACAACTCGAGGGGTTTTCGGCTGAATCTAGAAAATTGCTTCTC11.4492753623188406No Hit
ATATGATGAAAACAAAAGCATGAAGACATACTTTGGATGTAAAGAGCCTA11.4492753623188406No Hit
GAATGTAGGCTGCACACGGATCTGGCCTGCGGATGCCTTTTGTTGAATGG11.4492753623188406No Hit
CCACAATGTTATACAACAAAATGGAATTTGAACCATTACAGTCTCTTGTC11.4492753623188406No Hit
ATGAAAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGAATATTGT11.4492753623188406No Hit
TATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTACA11.4492753623188406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATAT50.0145.05
CTCCACC50.0145.01
CCACCAT50.0145.03
CACCATA50.0145.04
TCCACCA50.0145.02
CCATATA50.0145.06
GCTCTTT50.0145.0145
CATATAG50.0145.07
TATAGCT50.0145.09
ATATAGC50.0145.08
Produced by FastQC (version 0.11.5)