FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n02_FD_S4_R1_C04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_C04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAAAAAGTACAAGTAATATTGGTCTATTAAATATATATAATTTCTGGG22.5974025974025974No Hit
TAGCCCTACCTTTGATTGGGTTTGATCACACAGCTTCTTTAACTCAAATG11.2987012987012987No Hit
AAGTACTTCAGGTCAATGCTTGATAGCATATCTGCTGGTATTTGTGTTAG11.2987012987012987No Hit
TAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAAC11.2987012987012987No Hit
GGTAAGTCTTGATTGCCAATATTGCACATTTCAAACATGAATCCGTAGAC11.2987012987012987No Hit
TAATAAAACTTCACCCCTTTGCTGCTGCACCACCAGAACAGAGTAGGATG11.2987012987012987No Hit
GCCAAGAACTATAAACAATCCAAATGTCCATTAACTGGTGAATGAATAAA11.2987012987012987No Hit
ATCCTATAGGCAGGACTCTTACCAGGCCCCGTCTTTTAGGGACTATTAGG11.2987012987012987No Hit
GTCAATTGGTGTGGATGGCATGCCACTCTGCTGCATTTGAAGATTTAAGA11.2987012987012987No Hit
AAATCGGTCAATACTCACTACAACTCTCTCCGTGCTGGAGTATTCATCTA11.2987012987012987No Hit
GCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATC11.2987012987012987No Hit
GTACCTGACCGCCGAGATCCTGGAGTTGGCTGGCAATGCCGCTCGTGACA11.2987012987012987No Hit
GTTAGCATCAGAGTTCAGTGCTTTGGTCCTTGAAGTACAGGAATCAAGGC11.2987012987012987No Hit
CCATATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGT11.2987012987012987No Hit
GCTCAGCATATAGGTGCCCTTGGGGAGGCCCACAACTTCATAATAGAAGA11.2987012987012987No Hit
CATTCTATGGATGGAAAGAGCCTAACATACTCAAACCACATGAGAAATGC11.2987012987012987No Hit
GTTGGAATGGGATGCTTTTCTCCATTAAATACGGGCCACGGTGAATTACT11.2987012987012987No Hit
CATCCAGAGTTCCTGCAAGCTGTTAAGTCGCTTTTTAGCCATATAGCCAA11.2987012987012987No Hit
ACACATGAATACTACATAGCAACCATGGTTGGGAGCAGAAAGTACTGAGC11.2987012987012987No Hit
TGTTTAAGCATTTCACCCTCCAGAAGCCCCAGTAGCTAGGTAATCGTCAA11.2987012987012987No Hit
GTATTAATTGTCAGTGCTCGTATGAGATTGCCTCTCATATTCAGTCTTTG11.2987012987012987No Hit
ATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTC11.2987012987012987No Hit
GCACTGGAGGAACGCAACAATGCATTTTATTAAGGAAATTCCAGTCTGTG11.2987012987012987No Hit
AGGCAGCCTGATGCGGGGCTCGATCCCGGGACTCCAGGATCATCACCTGA11.2987012987012987No Hit
AGTCAACACTTATCAATTGATAATCAGGAACTGGGAAATTGTGAAAATTC11.2987012987012987No Hit
CCACTAATCTGCCTCCCAGGTGTTGCGAGATCCCTTCTTTTTAAATTGAC11.2987012987012987No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAAACT11.2987012987012987No Hit
TTATTATACAATAAAGACCTGATAGTTCTGGATTCTCAACAAAACTACAG11.2987012987012987No Hit
GGATGTATTGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCCCT11.2987012987012987No Hit
GTCCATTCATCTGTAAAATGGAGACAGTCGTAATACCTGTTCCTCAGGGT11.2987012987012987No Hit
GTACCAGGGAGGATAAGAGCTGAGGTATAAGCTGACAGGTGTTGTCATTG11.2987012987012987No Hit
GGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCA11.2987012987012987No Hit
AGTGAAAGCATCTCTTCCTCTGCAGTTGCTAGCAGGCTAGTCAAGATTCT11.2987012987012987No Hit
ATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGCCA11.2987012987012987No Hit
TTGTAGGAGTAAGGATGGTGGACATCCTTAGACATAATCCAACTGAGGAA11.2987012987012987No Hit
CTTCAAATATTTTCTTCTGTCTTTCCTTCCTTCTGGATTCCAGTTATACC11.2987012987012987No Hit
GCCCCCTTGTGACTAATGACAGGCTGGGTGAGACCTTGTCAATGATGGGG11.2987012987012987No Hit
CGCTTGATTTACAATACCGTAGGGATGGAGTCAGTCGCAAGTGCTGTTTA11.2987012987012987No Hit
CTCCAAAATAGTCCCTGTGGCACTCACTCACTAATGGGCAGAAGGATTCC11.2987012987012987No Hit
CCTGTTATATAATAGGATATAGGAAAGCCTTGAAAAAGATCAACTTTAGC11.2987012987012987No Hit
GGAATATATGAGAATACATGACATGAAACAATCCAATATGATCAAAAAAC11.2987012987012987No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC11.2987012987012987No Hit
GGTGTGAAGAGAGGATGGAGGTCACGGGAAGATTTTCCTGGGCACAAGAG11.2987012987012987No Hit
GAGCCTAACATACTCAAACCACATGAGAAAGGCATAAATCCCAATTACCT11.2987012987012987No Hit
AACTACTACAGTGCAGGTGTTAGGAAGGAGTTGAAAAAAGATGCATTAAG11.2987012987012987No Hit
TAAATGCACCAGTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAAC11.2987012987012987No Hit
GGTGTCTTTCAACCAGAATCTGGAATATCAGATAGGATACATATGCAGTG11.2987012987012987No Hit
AAACAAGGTGACGCACCAGAGGTCCCCCGAGGGATGCGGACGTTACACGC11.2987012987012987No Hit
CTCAAGCAGGCTCGATCAGGGCTTGACCCCATCACCCCAAGATCATGATC11.2987012987012987No Hit
GCGACATTAGAAATCACAAGCTTTTTTTATCTCTATGGATTTGTGGCTAA11.2987012987012987No Hit
CAAAAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAACCTCC11.2987012987012987No Hit
ATTGAAGAGCCATCTGGTCAGTTGCAGGACCAAGGTAATTGAATATCATG11.2987012987012987No Hit
AAAGGAAACTGAAGGAGTAATCAGAAATCTCCTAAAAAACAAGAAAGTCT11.2987012987012987No Hit
TAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGC11.2987012987012987No Hit
CCCTACCATTGATTGGGTTTGATCCCACAGCTTCTTTAACTGAAATCATC11.2987012987012987No Hit
GCTCTGGGAAAGCTGGGTACTGGCACTTCCCACCCTCCCCTGCCTGGACT11.2987012987012987No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAAGATTGAGGCCGAGTC11.2987012987012987No Hit
TTACACTCTCTTGTCCCTAATGCAACCAGAAGCCGGTACAGTGGATTAGT11.2987012987012987No Hit
GTCCAAAATGAATTCAATAAGGCATGTGAATTGACTGATTCAAGCTCGAT11.2987012987012987No Hit
AAATTGAATTTGGCAATCGGTGAAAATATGGCACAATAAAAAGTAGACTT11.2987012987012987No Hit
CGCTAACCGTGCCCAGTGAGAGAGGACTGAAGCGTACACACTTTGTCCAA11.2987012987012987No Hit
GGATAATGAACCAAGTGGGTATGCACAAACAGACTGAGTTCTAGAGGCTA11.2987012987012987No Hit
CTTTTGCATCTTTTTCGAAATGCCTAAAGACTTGTTGCATTCGGTACAGT11.2987012987012987No Hit
GCCATGGATGACTTTCAGCTGATCCCAATGATAAGCAAATGTAGGACCAA11.2987012987012987No Hit
CAAGTAACCTGTCCAACCAGACGCCAGTCTGTGACGGGACCACAACACAC11.2987012987012987No Hit
CTAGCGGGGCTGTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCAC11.2987012987012987No Hit
CTGTCGCCTTACTCCCTTGATGGTCTTTTGTGTCTCACTGTTGGCCACTT11.2987012987012987No Hit
CTTCAAAGATTTCTGCTATTAGAATAAGGTTCTGTTTTGTTAAGGTGGCA11.2987012987012987No Hit
GCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCATTCAATGCACC11.2987012987012987No Hit
GAAATGGCGCATGGAAATGAGTCGCTGCCTTCTTCAGTCACTTCAGCAGA11.2987012987012987No Hit
GAAGAAAATTGAGAAAATAAGGCCTCTTCTAATATATGGCACAGCATCAC11.2987012987012987No Hit
GGGCCAGACCTGTGAGGGGCTGCGGTGGGAGGCACTCTGTTCTGTCAGGG11.2987012987012987No Hit
GAACTTTGACCGCTGATCGCAAAGAGGCCAATCAGGCAATCTGAGGGGGC11.2987012987012987No Hit
CTCTAATATGAATCCCAGGTGTTGCGATAGCACTTCTTTTTAACTTGCCT11.2987012987012987No Hit
TTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACTTTTAG11.2987012987012987No Hit
GTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACT11.2987012987012987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAAG50.0145.07
GTATTTT50.0145.01
TTTTATA50.0145.04
TTATAAA50.0145.06
ATAAAGA50.0145.08
TATTTTA50.0145.02
TTTATAA50.0145.05
ATTTTAT50.0145.03
TAAAGAA50.0145.09
CTTTTTT50.0145.0145
Produced by FastQC (version 0.11.5)