FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_B09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_B09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCATTGGGATAATCTGAAAGTCATAAATGGCTGCACAGGATGCATTGA12.2222222222222223No Hit
GGCCAAGATGAGGAGAAGTGGGAGGAGCCAGTAGAGGGATGACCTCATGG12.2222222222222223No Hit
CCTGTGGATACCCACTAACTTGTGGGTCCTGTTGAATGTTTGCACTCATG12.2222222222222223No Hit
GGGAAGTACAGTGGTTATGTATTTGGATGTATTTTATAACTGTCTCTTAT12.2222222222222223No Hit
CCCCAGCCGACTACCCCGGCGGGGCAGAGCGCAGGGGAAGGATGGAAGGG12.2222222222222223No Hit
TCCTGGAAGAGAAGGTAATCGTGAAATTTCTCCAACTATTGCTCCCTCCT12.2222222222222223No Hit
GGTGAATGGCCAGTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTAT12.2222222222222223No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG12.2222222222222223No Hit
CCAGGCGCCCCTCAAAACAGACATTTTAATGATAGAGGAACAGAGCCAGT12.2222222222222223No Hit
GATATGTTCTCTTTTCTGGTTTTCCTATTCAGTCTCCAGAACACATACAC12.2222222222222223No Hit
GGAGTAACAGTGATAAAGCACAACATGATAAACAATGACCTTGGACCTGC12.2222222222222223No Hit
CCTCCAATCTGTCTGCACTCGCACAATTCCAAGAGAGATGCTAATGCGTC12.2222222222222223No Hit
CATGAGAAAGGCATAAATCCCAATTACCTCATGGCTTGGAAGCAGGTGCT12.2222222222222223No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAG12.2222222222222223No Hit
CAATCCCAGGATCGAGCTCATGACCCGAGCCAAAGGCAGCCGCCCAGCCC12.2222222222222223No Hit
GTTACTATATGTGAGAACCAATGGAACCTCCAAGATAAAGATGAAATGGG12.2222222222222223No Hit
CTACAGGAGGAGAAGTGAGAAATGATGATGTTGCCCAAAGTGTGATTATC12.2222222222222223No Hit
GAAAAGGATACACCATGGACACAGTAAACAGAACAAACAAATACTCATAA12.2222222222222223No Hit
TAGCCTGAAATGTTTCAAATTCCAGTGTGTTGTATAAAATTCTTGGCTCT12.2222222222222223No Hit
GGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGTGG12.2222222222222223No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA12.2222222222222223No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA12.2222222222222223No Hit
AAGATGCAAAGTACCGAAGACATCAAAAATCCGGAAAATATAGATGATAA12.2222222222222223No Hit
AAATCAAGGTCGCCAGACTTATGATTGGACATTAAACAGAAATCAACCGG12.2222222222222223No Hit
TAAAATAAGAGTACATGAAGTGTATGAAGAATTCACAATGGTTGGGATAA12.2222222222222223No Hit
CCCTAAGGCAACCAGCAGCATGTACAGTGGATTCGTAAGGACACTGTTCA12.2222222222222223No Hit
GGGGGGGGGGGGGGGTGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGG12.2222222222222223No Hit
CATGAAGCCTATGAAGAATTCACAATTGTTGGGAGAAGAGCAACAGCTAT12.2222222222222223No Hit
GATTAGTTCAACCAACAAGCAAGGTCTTATACAATACAGCCATTTTAGTT12.2222222222222223No Hit
TCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCAT12.2222222222222223No Hit
GTCTTTCATTCTACTACCATTAAAGAGCATAATAAGTCACTAATTTAAAA12.2222222222222223No Hit
CCTATATGAACTGATAGGGAAAAATTTCTAGAATAGATTGCAGCACTTCT12.2222222222222223No Hit
CTTTCAAAGAAAAAGTAGAGTAAGAGACAACATGACCAAGAAGATGGTCA12.2222222222222223No Hit
GTGCGGCTGTCTGGCTTTTGTGGCGTTCTCTCACCCCGGAGGTGCCCTAG12.2222222222222223No Hit
GTGTTTTTTTATGATGGGGACCTATTCTGTAATACATTCCCAAATCTTAT12.2222222222222223No Hit
TCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCAGATGCAGGTC12.2222222222222223No Hit
GGTCTATCACGACCAGTGAGTACCCTTCCCGTTCAAAGTCATGCCCGCTT12.2222222222222223No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAAATTTCACAATTTCCCAGT12.2222222222222223No Hit
GTTTACGACAGAGGTGCCATCATCACCTGTTGCATTGGTGGCTGACTCTC12.2222222222222223No Hit
GGATGGACGGATGGACAGACGCTTTTCCCCGGCCCTCCAGAGGCGCCCTA12.2222222222222223No Hit
GGACAAGATGTTGCGCCGAATCAACATTACGGAACCATGAGGAGGTACTA12.2222222222222223No Hit
ATAAAAAGACTTCAATGTTGTTGTACAATCAAGTTACTCCCTCAAGATAA12.2222222222222223No Hit
GAATCCCTCCCAACACAATCTCTTTCATTTAAGTCACAGAGCTGCGCGAA12.2222222222222223No Hit
CATTCGATTCTGGTAGGCCTGCAAATTTTCAAGAAGGACATCTTTCATAC12.2222222222222223No Hit
CCTCAAGAGTTCGTGCATGGGGTTAAGTCGCTGGTTTGAAATATTGACAA12.2222222222222223No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)