FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCCCCAGCCTTGA15.555555555555555No Hit
GCGATAGACCTTCTTTTTAACTTGCATCTCTAAGCATAATTGGTCATCTT15.555555555555555No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA15.555555555555555No Hit
ATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCACA15.555555555555555No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG15.555555555555555No Hit
ATGTAGAAGGATTCGAGCCGAACGGCTTCAATGAGGGCAAGCATTCCCAA15.555555555555555No Hit
CATCGGAGGAATTGAATGTAATTTTAAAACGATTAGAGACTATTAAAATA15.555555555555555No Hit
AGAATGTATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT15.555555555555555No Hit
CTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT15.555555555555555No Hit
ACTATTCCCCCGTGCTAACAGTCCCTTTGAGGACTCAGCCATCCATTACA15.555555555555555No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT15.555555555555555No Hit
CACCTGGAGACACATCAGCTCACAGCACGTACCGTGGCTGAAGTGCAACA15.555555555555555No Hit
TTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTC15.555555555555555No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT15.555555555555555No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTGATTTTC15.555555555555555No Hit
ACTACAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGGCCTCTTCTA15.555555555555555No Hit
AAAGAAATACACAAATACAATATACTGGTGGGATGGGCTACAATCATCCG15.555555555555555No Hit
CAATAATTTTGGCCATGGTATTCTCACAGGAGGATTGCATGATCAAGGCA15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)