FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAATCTCCCACAAAATAGACTATGAGTAAGATATATTACTGACTCAA14.761904761904762No Hit
TACTCCAGCAGGCTAAGACAGGAAAGAAGATCTTGATCAAACCAACATTA14.761904761904762No Hit
GGATGGCTCCCATCCTAATTCCACAAATAATTGTGCTGAGGCACATATAC14.761904761904762No Hit
GGATAAGTTTGGACCTCCATCTGATACTAATGGCCCTAACTTGGATTGGG14.761904761904762No Hit
GCACTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCT14.761904761904762No Hit
ATCTGGGTCTTCAGTTAAAGAGCCAGCATCCTTTACGAGAACTGTGATTC14.761904761904762No Hit
GTCGATAGGTGCAACTCCCCAATTTTTAAAAAGCACTTTCGCATCCTTCT14.761904761904762No Hit
AGAGAAATGAGCAACGACAAACTTTATGCACTAAAATGAATGAAGCAGTA14.761904761904762No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAG14.761904761904762No Hit
GATTTTATGAGTATGGCTTTTGCTATTCTACACACAATTGTTAAATCTTC14.761904761904762No Hit
GCAGTGGAGGTTGATGGGACACCTGACTTGTAACAGTGTCCCATTGGCTG14.761904761904762No Hit
CTATACAATAAAATGGAATTTGAAACATTTCAGTCTTAAGTACCTAAGGC14.761904761904762No Hit
AGTCCCAAAAGATCTCATTGCATGTACCAAATGCCTTGTATGAATAGCAA14.761904761904762No Hit
CTCCTCAGAATCGACCTACTCAGGAACTTGAAGGTGCCGTTTATGATTGG14.761904761904762No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT14.761904761904762No Hit
GCCTTTGGCCTAACGGGGGTGGCATATGTAAGGTATGCTCTTGGAAGGAG14.761904761904762No Hit
TACCAAACATTCCGATCACCTTGTCGATAGTTTCAACAAAGCAATTTAGA14.761904761904762No Hit
ATCTCCCACATCATTGACGATTAGTAAGTTATAGTCAGTTTCTCTGATCC14.761904761904762No Hit
ATCCAGAATATACAATCTAGATACCAAAATTGGGCCATTGAACGATACAT14.761904761904762No Hit
AGGAGACACACGGTCGTTTGTCCACTATTCGACCAGCAGGGCGGGCAAGA14.761904761904762No Hit
CACACAAAGGGAAGCACATAATGTAAAACCTAGACGTAACTGAACCTAAA14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)