FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S4_R1_A09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S4_R1_A09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCATGCTCATGCCTAGGCAAAGGATAATAGGCCCTCTTTGCGTCCGCA11.3157894736842104No Hit
AGCCAAAAGCATGGAATATGATGCCGTTGCAACTACACATTCCTGGATTC11.3157894736842104No Hit
GTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTC11.3157894736842104No Hit
GGAATGTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCAT11.3157894736842104No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC11.3157894736842104No Hit
ATGCAATCATATGCATGAAGACATTCTTTGGCTGGAATGAGCCTAACATA11.3157894736842104No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG11.3157894736842104No Hit
ACATATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCAA11.3157894736842104No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA11.3157894736842104No Hit
GTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCAACGGCCCA11.3157894736842104No Hit
GTCAAGAAAGAAGAACCAGTGCTAACGGGCAACCTCCAAACACTGAAAAT11.3157894736842104No Hit
GAGTTAAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTATTAGT11.3157894736842104No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTAGCA11.3157894736842104No Hit
CCTCCATGGCCTCAGCGGCCTGTTCACTCGAACCAGCCATCTCTTCCCTA11.3157894736842104No Hit
CCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGA11.3157894736842104No Hit
GGTGTAGTTGCAACGGCATCAAATTCAATGCTTTTGTCTGGACCATGGGC11.3157894736842104No Hit
CAATAGTAAGTTCCCTCGGTGATCTATAACCCGGAAAACGCTGTCACTAC11.3157894736842104No Hit
GGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTGGTCTG11.3157894736842104No Hit
ACCTAAATGTGAATTGTGAACCTAACAAAATGGATAGAGCAGATAAACTA11.3157894736842104No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT11.3157894736842104No Hit
CATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTC11.3157894736842104No Hit
CCTTCATTGTTACCCCTGAATGCTGCCATAACGGTTGATCAATCAAAAGG11.3157894736842104No Hit
CATTGATTCCATTACATCCTGTAAGAAATCTATTAGCCGTCCTGACTCAT11.3157894736842104No Hit
CTCCAGTTGTGGAGTGGCTGATAGACGGTTGGTTACTGTTTTTGGCGAGG11.3157894736842104No Hit
CATGAAGACATTCTTTGGCTGGAAAGAGCCTAACATAGTCAAACCACATG11.3157894736842104No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA11.3157894736842104No Hit
CATCAGGCTGAACACTTGGCTGTTTTGGAGTAATTTGAATTTGTCTATCC11.3157894736842104No Hit
AATCAGAAGTGACCCACTGCAATCCTACTCAATAAATAATCAACGGATTC11.3157894736842104No Hit
TATCACTGTTACTACAATACTCGTGTAAGCTGATTCATTTACTCCAGACA11.3157894736842104No Hit
TGGTGGAATAGGAATGGCCCAAAACCAAGTACAGTTCATTACCCTAAGGT11.3157894736842104No Hit
CGCATATAGACTTTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTG11.3157894736842104No Hit
TGATTATGGAAGCTGTTTTCCCAAATGAAGTGGGGGCAAGAATACTGACA11.3157894736842104No Hit
CAGGATGCATTGAGCAAGGCCGTATTTATGTAAACTCCCTTCATTATGTA11.3157894736842104No Hit
GCGCAGGGTGCCGGTTGGGGGTGGGGCCGTTTTGTGTTGTAGGGTGGGCG11.3157894736842104No Hit
GTCTACGGCTAGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATAC11.3157894736842104No Hit
CCTCGGGTCTTAAATACTTAATTTCAGATAATCAATAAGAAGGAAAGTTT11.3157894736842104No Hit
CTATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCC11.3157894736842104No Hit
GAACGAAGCTGGTTCAGAGCCGCACATCGAAACTACACAGGGGGATGCAA11.3157894736842104No Hit
AGTAGAAACAACGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT11.3157894736842104No Hit
GGCAAGAATTGTGGCAAAGGTAATATCAAATCAGTATTTCTTGAGCAAGT11.3157894736842104No Hit
TATGGCCCCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCC11.3157894736842104No Hit
GTCTAAGCATCAGGGCCAAACAAACAGCACCTATCATTCACCTCAGGGAG11.3157894736842104No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT11.3157894736842104No Hit
TAGTCAAACCACATGAGAAAGGCATAAATCCCAATTACCTCATGGCTTGG11.3157894736842104No Hit
CTAGAGATACTCACTAAGACCACAGTGCAACATATGGACATAATCAAAAA11.3157894736842104No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT11.3157894736842104No Hit
CCCCTATTGAACCTATATGTGTATCTGTAGTCTATGATGAACAATTGAAG11.3157894736842104No Hit
AAGGATGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCATGAGATT11.3157894736842104No Hit
TTCCCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTT11.3157894736842104No Hit
CGCTTATCTGTTGTTCTACTTCAAGCAGTAGTTGTAAGGCGTGCATAAAT11.3157894736842104No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGATACTATTCTCTGTC11.3157894736842104No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAAAAC11.3157894736842104No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATAGCAATGGAT11.3157894736842104No Hit
ATGCTTAATGCTGGGCCATATCTCTGGTCTTCTTTGCCCAAAATGAGAAA11.3157894736842104No Hit
ACTGGGATGCAGATTAGAGGTTTAGTATACTTTGTTGAAACTTTAGCTAG11.3157894736842104No Hit
GGACCACCCAAATTTAAAAAGATATCCTTCCAACAAGACACGGGGAAGAG11.3157894736842104No Hit
GTCCCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCT11.3157894736842104No Hit
AACACCACTTGGGTAAATCAGAAATATGTTAACATCAGCAACACAAACTT11.3157894736842104No Hit
CTATTAGAAGAGGCCTTATTTTATCAATTTTCTTCTTTGTTGATTCATTG11.3157894736842104No Hit
TCGTATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTT11.3157894736842104No Hit
ATCAAACTTTGGTCAACATCATCATTTCTCACTTCTCCTCCTGGAGTGTA11.3157894736842104No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTGTTTCTTCCCTATTGT11.3157894736842104No Hit
GCACTCCATACTTACAAAGTTCACCACATCAGTATCATTTCTCAAATGAG11.3157894736842104No Hit
GAATTTGTGTGTCTCCCCTATGGCACCTATATGTTTATCTCTAGTCTTTG11.3157894736842104No Hit
GGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCAT11.3157894736842104No Hit
GATCTGGGCTCTGGCTCATCACTGTCATACTGTTTAAGGTCTCCAACATC11.3157894736842104No Hit
GTATTATATACCTTAGGCTAATGAACTGTACTTGTTGTTGGGCCATTCCT11.3157894736842104No Hit
CCTATATGACCTGCTTGGGAAAAATTTCTCGATTAGCTTGCAGCACTTCT11.3157894736842104No Hit
AGCTAGGAGCAGATGCGAAAAGCTTGAGCATTCTGGTCTCCCAGTAGGGC11.3157894736842104No Hit
CATTTTGGACCCAGCTTGCTAGAGATCTGGGCTCTGGCTCATCACTGTCA11.3157894736842104No Hit
CTCATTAGGCTTCGATATGGGCTCCTGTCTTTAATGTTTCCATTGGAATT11.3157894736842104No Hit
CTGCCTAAGGATGTCCACCATCCATACTCCTCCAATCTGTGTGCTGTGGC11.3157894736842104No Hit
CCATGAGGGAATACAAGCAGGAGTGGACAGATTCTCCAGGACCTGCAAGT11.3157894736842104No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA11.3157894736842104No Hit
GTATACAACAACTACCATTTAGCTGACTCTTATACCTAACTGCGACATCT11.3157894736842104No Hit
ATCATAAGTCTGGCGACCTTGAGTTAGTTTATCTACCCTTGTTTGTTGAA11.3157894736842104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAT50.0145.04
TTATTAG50.0145.02
ATAGCCT50.0145.09
CTTATTA50.0145.01
TCCATTA50.0145.0145
TATTAGA50.0145.03
TAGATAG50.0145.06
GATAGCC50.0145.08
AGATAGC50.0145.07
TTAGATA50.0145.05
Produced by FastQC (version 0.11.5)