FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTATCTGCAGAAGAGACTGAAGAAGTCAGCGCCGCATTTCCATGCC14.0No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT14.0No Hit
GGAGTATTCATCTACTCCCATTTAACTCACTCATATCCCTCTCAGCGACA14.0No Hit
TAATTAATAAGTCAATACTCGATAGCTTAATATAGCCCCCCAAGATCGAA14.0No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT14.0No Hit
TTAGTAAACTATCTTACAACTATAGATATGATAAATAGCGAAAAACTAGA14.0No Hit
CTCCAACACCATGTCAAGCTTTAATGTAGACTGTTTACTTTGGCATATCC14.0No Hit
GGCCAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGA14.0No Hit
GATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGA14.0No Hit
TTTTTGGACAGTAGGGATAGAAAAGAGTCGCATGGCCACAACTACGGCAG14.0No Hit
AATCAACGGAGAAACAACGATAAAATGACAATTCTACAAATATGGACCAC14.0No Hit
TGGGTAACGATCACATTGAAAATTTAGCGATGACGGCATCTGCTCCAATT14.0No Hit
TCTCAACCTACTTCTCGCAACATTTCTGAAATCACCCACGACCAAATTTC14.0No Hit
GGTGACCCGAATGCTCTATTGAAGCACCGATTTGAGATGATTGAAGGAAG14.0No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTGTAGCTAGGAGCATTTGCG14.0No Hit
GTATCCTCAACCCAGATCCTCTCACATTCACATTCAATGAGGAAAATTGC14.0No Hit
GTGCAGCAGCTACTGAAAACAATATGGCAGTTCATCAAAAAATTAAAAAT14.0No Hit
TTCCTTGGTCAGGTATTTCTCTTTCACAGAAGCCTCGGCCTCAATCATGA14.0No Hit
CATATACCACAGGATGGGCACAGTGACAACAGCAGCTGCTTGTGGTCTAG14.0No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA14.0No Hit
CTACAGATACAAAAATAGGTGCCCTAGGGTCGACACACAAAATCAGACGA14.0No Hit
CCGATATTACCGAGAGACAGCCAACAAAATAATATCTGAGAAGCCACGCA14.0No Hit
TAAGGACACTGTTCCAGAAAATGCGGGATGTGCTTGGGACATTTGACACT14.0No Hit
GAATTATGCACAAGATTACAGAGACGAATGAACAAGGACACACACTCTGG14.0No Hit
CAGCGACATCTCATTGCTTGGGGTCATGTCGGTCAGTATTCCTATCATTC14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)