FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158568
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT16740.14448871365340663No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC16410.14164036983586636No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG16100.13896465291635882No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA15940.13758363773209686No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT14610.1261039490129194No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA14560.12567238176783752No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA13910.12006200758177338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCG12200.030.296169
GTATTAC14150.030.1963181
AGCGAAA3600.030.174991
ATATATG11750.029.5907213
TTACATC14650.028.678824
CAATTCG3301.8189894E-1228.5500359
TATATGT14150.027.6444684
TATAGAC17050.027.608792
TACATCA14800.026.9221275
TATACAG14300.026.8502465
TATACGT1907.2038547E-626.687964
TAACGAC4350.026.6429843
TATATAT13600.026.0924932
ATAGACC7300.025.7989863
TATTACA17150.025.758712
GTATGTA12150.025.6489419
CGAAAGC4800.025.6487452
ACATCAA16550.024.0764166
ATTACAT16500.023.7062023
GTATATA26050.023.630321
Produced by FastQC (version 0.11.5)