FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_H06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_H06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG50.2762430939226519No Hit
GAGGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGA40.22099447513812157No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA40.22099447513812157No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40.22099447513812157No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG30.16574585635359115No Hit
TATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACGG30.16574585635359115No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT30.16574585635359115No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG30.16574585635359115No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG30.16574585635359115No Hit
ACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGGAGGAGAAGTGAG30.16574585635359115No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30.16574585635359115No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA20.11049723756906078No Hit
TATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGG20.11049723756906078No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT20.11049723756906078No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG20.11049723756906078No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA20.11049723756906078No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA20.11049723756906078No Hit
ACACGGAGGGAAGTCCACATATATTACCTAGAGAAAGCCAACAAAATAAA20.11049723756906078No Hit
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT20.11049723756906078No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG20.11049723756906078No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC20.11049723756906078No Hit
TTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTA20.11049723756906078No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20.11049723756906078No Hit
GTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAATAT20.11049723756906078No Hit
GGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAATGAGAA20.11049723756906078No Hit
AAAATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGC20.11049723756906078No Hit
GCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGGATAAGAGTCA20.11049723756906078No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC20.11049723756906078No Hit
ATTCTCTTTCTAGCATGTACGCCACCATCAAGGGAGCAATTTTACAATCC20.11049723756906078No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG20.11049723756906078No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA20.11049723756906078No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG20.11049723756906078No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT20.11049723756906078No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT20.11049723756906078No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG20.11049723756906078No Hit
GAGTCACAGCTGGCAATAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTG20.11049723756906078No Hit
ATTCAGTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAACATGCCCA20.11049723756906078No Hit
TCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAA20.11049723756906078No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC20.11049723756906078No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG20.11049723756906078No Hit
ACATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAG20.11049723756906078No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20.11049723756906078No Hit
GGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAATAGGG20.11049723756906078No Hit
AGCCGAGATCGCGCAGAGACTGGAAAGTGTCTTTGCAGGAAAGAACACAG20.11049723756906078No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT20.11049723756906078No Hit
CTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAAGCAAATGTA20.11049723756906078No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA20.11049723756906078No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC20.11049723756906078No Hit
AATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAAC20.11049723756906078No Hit
GATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTT20.11049723756906078No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC20.11049723756906078No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT20.11049723756906078No Hit
CCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTC20.11049723756906078No Hit
GTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGATGC20.11049723756906078No Hit
CCAATCCACTAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCACTACG20.11049723756906078No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20.11049723756906078No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTC20.11049723756906078No Hit
CTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTG20.11049723756906078No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA20.11049723756906078No Hit
CTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGCATT20.11049723756906078No Hit
CGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTAT20.11049723756906078No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG20.11049723756906078No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.11049723756906078No Hit
ACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAACAAAGACT20.11049723756906078No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20.11049723756906078No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT20.11049723756906078No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT20.11049723756906078No Hit
ATGTTCAATCGGGGCAACATCTTCTCCTATTTCATCAAGTTCTATCCAGC20.11049723756906078No Hit
AAAATTAGCCACAAATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATG20.11049723756906078No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)