FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_H04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_H04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCG21.4492753623188406No Hit
GAGGTGGGGGCGGATTGGGGGGAGGGGGGGAGTTCGGGCGCGGGGGGTGG10.7246376811594203No Hit
GCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCACTCGAT10.7246376811594203No Hit
AGCGAGACACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCT10.7246376811594203No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG10.7246376811594203No Hit
TCTCTAACACTTAAAAATCGGTCAATACTCACTACCACTCTCTCCGTGCT10.7246376811594203No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT10.7246376811594203No Hit
GTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTCCAATTTCCTT10.7246376811594203No Hit
CGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCA10.7246376811594203No Hit
ATTGAAGGAAGACACCGGATAATGGACTCGACAGTGTTGAACAGTATATG10.7246376811594203No Hit
CAATACAACTTGTCAAACACCCAAGGGTGCTATAAACACCAGCCTCCCAT10.7246376811594203No Hit
GGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCTC10.7246376811594203No Hit
CCATCATTCTTATAACTTCTGTTCGCATGTCGGATGTCCGGCCTTCTTTG10.7246376811594203No Hit
GGGGGGGGTGGGGGGGGGGGAGTGGGGGGGGGGGGGGGGGCGGGCGGGGC10.7246376811594203No Hit
CTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCACATTCTTTCAT10.7246376811594203No Hit
GACAGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATC10.7246376811594203No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG10.7246376811594203No Hit
TGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTT10.7246376811594203No Hit
GGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGTACATCTGTTCATCC10.7246376811594203No Hit
GTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCG10.7246376811594203No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT10.7246376811594203No Hit
AGTTAAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTATTAGTA10.7246376811594203No Hit
TTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCT10.7246376811594203No Hit
AACAAGTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGG10.7246376811594203No Hit
GCTGATCCCAATGATAAGCAAATGTAGGACCAAAGAAGGAAGACGGAAAA10.7246376811594203No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGATG10.7246376811594203No Hit
GCATTTGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTCTGGTT10.7246376811594203No Hit
CCCTGCAGTGGGACACTTCTGCTGTAAAATAGTCCCTCCTCATGCTTGCG10.7246376811594203No Hit
TTGCAGGGGGGCTGCGCGGGGGTGTGCGGCGGCTGGGGGGCGTGGTGGGT10.7246376811594203No Hit
GGACTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCATATTA10.7246376811594203No Hit
AATGGGAGTAGATGAATACTCCAGCGCGGAGAGAGTGGTAGTGAGTATTG10.7246376811594203No Hit
CCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGT10.7246376811594203No Hit
AGAAAGAAGAAGAAGTGCTAACGGGCAACCTCCAAACACTGAAAATAAGA10.7246376811594203No Hit
CGTTTGAGGCGGTTGGCGTGGCAGGTGGGGTGGGGGGAAAGGAGGTGGGT10.7246376811594203No Hit
ATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGACCCG10.7246376811594203No Hit
GTGTTAAACACCAGCAGAGCCGCGATCCCAACGCGTCCATAATAGGTGGA10.7246376811594203No Hit
GGCCTCCGCTGCCTGTTCACTCGATCCAGCCATCTGTTCCATAGCCTTTG10.7246376811594203No Hit
ATCCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAG10.7246376811594203No Hit
GTTTGGCGCCAAGCAAACAATGGCGAAGATGCAACAGCAGGTCTTACTCA10.7246376811594203No Hit
GGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCC10.7246376811594203No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA10.7246376811594203No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC10.7246376811594203No Hit
ATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGA10.7246376811594203No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT10.7246376811594203No Hit
CGTTTAATCATTCTGATTAACTCCATTGCTATTGTTCCAACTCCTTTCAC10.7246376811594203No Hit
ATTCACTGGAGAGGAGATGGCCACCCAAGCGGACTGCACCCTTGACGATG10.7246376811594203No Hit
TCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGC10.7246376811594203No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT10.7246376811594203No Hit
TCTCCCAACCATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCA10.7246376811594203No Hit
GGGCCGGCGGGGCGTGGGCGGGGAGTGTGGGTGTGTGGGGGGTGGCGGGG10.7246376811594203No Hit
AGGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT10.7246376811594203No Hit
CTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATTTAATGTCAG10.7246376811594203No Hit
CGATTTGCAGACAATGGATTGGGTGATGACCCATTCCTTGATCGGCTCCG10.7246376811594203No Hit
GGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATT10.7246376811594203No Hit
TTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCATG10.7246376811594203No Hit
CCAGTATATTGTCTTGGTGAATTTCTTTTGTCCAAGATTCAGTATCGAGA10.7246376811594203No Hit
GGTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGA10.7246376811594203No Hit
ATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTATG10.7246376811594203No Hit
GAGAATAGCTGTTGCTCTTCTCCCAACCATTGTGAATTCTTCATACCCTT10.7246376811594203No Hit
CAATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCT10.7246376811594203No Hit
ATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATT10.7246376811594203No Hit
GGGGCAGGTTGCCGAGGAGGGGGGAAGGTGCGTCCAGGCGGGGGGCGTTG10.7246376811594203No Hit
CATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAAC10.7246376811594203No Hit
TTGTGGCCTGGGGCCTGGGGTAGGTGGTGGCGGCGCAGGGGTTGGGTGCC10.7246376811594203No Hit
TAAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTATTAGTATCA10.7246376811594203No Hit
CTTGCACCCACTTCATTTGGGAAAACAACTTCCATAATCACATCCTGTGC10.7246376811594203No Hit
ATTTAAAGCAGAATAAAGGAATGTTAAGATTCCGTATATTGTATAACTTT10.7246376811594203No Hit
TTTGTCTCCCGGCACTCCTAGTGTCCAGACAAAGTTCAGTCTCCCAACTC10.7246376811594203No Hit
CTTCTGTGGTCACTGTTCCCATCCTGTTGTATATGAGGCCCATGCAACTG10.7246376811594203No Hit
CTCTAGACCTAAAGCAGCTTCTGTGGTCACTGTTCCCATGCTGTTGTATA10.7246376811594203No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG10.7246376811594203No Hit
TTTATGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10.7246376811594203No Hit
CCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTGAAAATG10.7246376811594203No Hit
CATTTCTGCTGGTATTTGTGTTCGAATCTTCATTCTTTTACTCTCGAACA10.7246376811594203No Hit
GAGGCGACCAATTGAGGGGCCAGCTCAGAAGAGTCTATTGGTGTGGATGG10.7246376811594203No Hit
GTCCTACATTTGCTTATCATTGGGATCAGCTGAAAGTCATCCATGGCTGC10.7246376811594203No Hit
GGATTCAATTCCCCAGTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAAT10.7246376811594203No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA10.7246376811594203No Hit
TCCTCAACCCTATTGCTGCCTTGCATATGTCTACGGCTTGTTCCTCAGTT10.7246376811594203No Hit
AATTGCTGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT10.7246376811594203No Hit
CAATGGAGTTAATCAGAATGATCAAACGTGGAATCAATGACCGAAATTTC10.7246376811594203No Hit
GATCAGAATGGTGCTGGCTAGCCCTGCGGGAATGGCGATGGGACAGAGGG10.7246376811594203No Hit
CTCCATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGC10.7246376811594203No Hit
GGCAATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGC10.7246376811594203No Hit
CTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACCAGCAC10.7246376811594203No Hit
TGCTTGTATTCCCTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCGT10.7246376811594203No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT10.7246376811594203No Hit
GGGGGGGGGGTCGGGGGTGGGGGGAGGGGGGGGGGGCCGGGGGGGGGGGG10.7246376811594203No Hit
TCAATGTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATT10.7246376811594203No Hit
GCCTTAGGGACAAGAGACTGAAATGGTTCAAATTCCATTTTGTTGTATAA10.7246376811594203No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG10.7246376811594203No Hit
GTGGGGCTGGTGCCGGAGGGGGGGCGGTGGGGGGGGGGGGGGCGGTGGGG10.7246376811594203No Hit
AATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAAC10.7246376811594203No Hit
CTTTTGGAAGGTAGATTCAACAAATATGTTATCAAGAGCACGCACTCACG10.7246376811594203No Hit
ATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA10.7246376811594203No Hit
CAAAATGACCATCGTCAACATCCACAGCACTCTGCTGTTCCTGTTGATAT10.7246376811594203No Hit
ATGTTGCCCCGATTGAACATATCGCAAGCATGAGGAGGAACTATTTTACA10.7246376811594203No Hit
ACTAAGACCACTGTGGACCATTTGGCCATAATCTAAAAGTACACAGCAGG10.7246376811594203No Hit
GCGGATCATCATTCATGTAAGAAGAAGATGTGCTACCAGGCAACCTGCAA10.7246376811594203No Hit
CTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGTGA10.7246376811594203No Hit
GTAATGGTGCATTTTCTCCCGCGATTGCTCCCTCCTCAGTGAAAGGCCTT10.7246376811594203No Hit
AACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGACAT10.7246376811594203No Hit
AAACAAGAGATACTCCAAACAGAGAGGGCAGACGAACCGGAAACACAATG10.7246376811594203No Hit
GGTCAAGAATCTCAACTAGGCTTTTCACTATAAGTCTAGAACTGCCCAGG10.7246376811594203No Hit
GATCGCGCTGGGGTGTGGGGGGTGGGGGGGGGGGGGTTGAGGCGGGGCGG10.7246376811594203No Hit
GCTTGCTAGAGATCTGGGCTCTGGCTCATCACTGTCATACTGTTTAAGGT10.7246376811594203No Hit
GGCTCCAATCATCCGACGATTTTGCTCTCATAGTGAATGCACCAAACCAT10.7246376811594203No Hit
ACATACCCACCACACGCCGAGAGCATGCTCTGTTCTGCCGCCCAGAAGCT10.7246376811594203No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10.7246376811594203No Hit
GGTATGAAGAATTCACAATGGTTGGGTGAAGAGCAACAGCTTTTCTCAGA10.7246376811594203No Hit
GACCCGAGTCACGAGGGGGAGGGAATACCACTATATGATGCAATCAAATG10.7246376811594203No Hit
GTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTT10.7246376811594203No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC10.7246376811594203No Hit
ATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTGCATC10.7246376811594203No Hit
GGCCAGGGGTGGCGGAGGGGCGCCGGTGGAAGGGGCGGAGGGGCGGGGTT10.7246376811594203No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT10.7246376811594203No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA10.7246376811594203No Hit
GTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAA10.7246376811594203No Hit
GTATTTATGTACACTACCTTCATTATGTATTCAGTAGACCTGCAGTCTGA10.7246376811594203No Hit
GTCTTCATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTG10.7246376811594203No Hit
GAATGAGTGCTGACCGAGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT10.7246376811594203No Hit
AATAAAAATTCTCCCATTAGATGCTGCCACAACAGAACATAGTAGGATGC10.7246376811594203No Hit
CTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGC10.7246376811594203No Hit
GTATCACATCCAGATGGCTACTACCACCCATCAAATAATACGGAATGAAA10.7246376811594203No Hit
TTTCATGCCTGATTAGTGGATTGGTGGTAGTAGCCATCTGTCTGTGAGAC10.7246376811594203No Hit
GAAGAAGACGGTCTGCACCGACAGCATTGACACGATGGTCAGCAGCTAGT10.7246376811594203No Hit
TCCCACCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTAT10.7246376811594203No Hit
ACTTATTCGTCATCAATGATGTGGGAGATCAATGGCCCTGAGTCAGTGCT10.7246376811594203No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG10.7246376811594203No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC10.7246376811594203No Hit
CTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTGCCT10.7246376811594203No Hit
GAACTTGATGAAATAGGAGAAGATGTTGCCCCGATTGAACATATCGCAAG10.7246376811594203No Hit
CCTAGACAAAATTGACTCCACCAAGCTAGAAATTAGACCCCGGCGCCTAT10.7246376811594203No Hit
CTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGC10.7246376811594203No Hit
GCTTCAGGGACATACGGATCTACAGAAGCAACAAGCACCATAGGTAAAAT10.7246376811594203No Hit
CATCTTTTCATTCACTGGAGAGGAGATGGCCACCAAAGCGGACTACACCC10.7246376811594203No Hit
TCTAGGGTGTTGGCCAATGCCGGTGCGGCCGGTTGAGTTCTGTTGAATGT10.7246376811594203No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers