FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCAGATTTTATTTTGTTGGCTTTCTCTATGTAATATATTTGGACTT12.857142857142857No Hit
TTCAGTCTCTTTTACCTAAGGCAAACAGAAGCCGGTACATTGGATTCGTA12.857142857142857No Hit
ACTCTCATATTGAAGACACATATACATCTCAGCGACATCTCCTTGCTTGG12.857142857142857No Hit
ATTCACCAGCATCATTTCAAAGACCTGTAGGTCGTTTGGTTCCCTATTTT12.857142857142857No Hit
GAGCAATTGTCGAAAGGATGTTTATCAGGTTGTTTCTGTATAATCATGAC12.857142857142857No Hit
GTATAAGTTTTGTACAACATCTGATACTAATAGCCCTACCTAATATTGGG12.857142857142857No Hit
TCCCATCACATTGTCGATGGATTCAAATCACCATTTATGCAAAATCACTT12.857142857142857No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC12.857142857142857No Hit
ATCTTCATGATCGCAGAGAACTCGTCTTTCTTGCGCCGGCCCGACGCGAA12.857142857142857No Hit
CCATGCATGTACGCTGATTTTCAGTGTGTGGAGGTTGCCCGGTAGCACTT12.857142857142857No Hit
ATGACGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTTCAGC12.857142857142857No Hit
GAAAAAGTACACTAAGAACAATGAAAAGATGTACCACACCTAAAACAGTA12.857142857142857No Hit
CATTATAGGGGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGAGTTC12.857142857142857No Hit
TTGTTTTCCCAAATGAAGTGGGGGCAAGAATACTGACATCAGAGTCACAG12.857142857142857No Hit
GTCAGTAAGTAAGCTAGAGTCCAGTTTTCGTTGAATTTCCCACAATACGG12.857142857142857No Hit
TCCCCTGAATAACTTTGTCCGTCATAAAGAGATTGATTATTTAATCAATG12.857142857142857No Hit
CCGGATGGGGATGATGAATTCGCAATGGTTGGGAGAAGCGCAACAGCTTT12.857142857142857No Hit
ATATGAACCAATCAAATGAATGAACACATTCAAAGGCTGGAAAGACCATA12.857142857142857No Hit
GCTGTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAAC12.857142857142857No Hit
CAGTTCATAACCATAACGAATAAAAAACTTATTTCGAAAAGGTAGAAATT12.857142857142857No Hit
CTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTATTATC12.857142857142857No Hit
GGGAAAAATACTGTGTTCTTGAAATAGGAGACATGCTCTTGAGGACTGCG12.857142857142857No Hit
GAATTGGAGATGATCTTGACCCGCTTGCAGATATCGAAAGCATGTGGTGG12.857142857142857No Hit
AGTGATAGAAGAGATACAGGACATAGAAAAAGAAGACAAAATCCCAAGAA12.857142857142857No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCGATGTTTGTATTCATTACAAA12.857142857142857No Hit
ATTCATACGCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT12.857142857142857No Hit
TCCATCTACCATACCTGACAAACCACCTTAAATGAAAACGGCAATGTCCC12.857142857142857No Hit
CTCCTAGATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT12.857142857142857No Hit
CGTACTGATCCAAAATCGAAGACTAAGTGCGACAATGCTTCAATACAATG12.857142857142857No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA12.857142857142857No Hit
TCTTCTAACCGAGTTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCC12.857142857142857No Hit
AGTGACTCGGGTCTTCAATACATAATTTAAGAGCATACATCAGCAGGAAC12.857142857142857No Hit
GCACAAAACTAAGTTGAATTAAAATCAAACTCCTCGAATGTTCCTGGAGC12.857142857142857No Hit
ATGCTAGAGTCCCGTGTTCGTTTAATTACCCCCACAACCGCCCCATGCCC12.857142857142857No Hit
GAGTAAATGAATCAGCGGACATTAGTATTTGAGTAACAGTGATAACGACA12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)