FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_H02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_H02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG50.4930966469428008No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG30.2958579881656805No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA30.2958579881656805No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA30.2958579881656805No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGT20.19723865877712032No Hit
ATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATG20.19723865877712032No Hit
CTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGGCTAATAGATT20.19723865877712032No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC20.19723865877712032No Hit
ACTTATGATTGGACATTAAACAGAAATCAACCGGCAGCAACTGCATTGGC20.19723865877712032No Hit
CCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAAT20.19723865877712032No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC20.19723865877712032No Hit
GTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGA20.19723865877712032No Hit
AATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGAGGCA20.19723865877712032No Hit
CTCCTATTTCAAGAACACAGTATTTTTCCCATTTGTGTGGCTCCAGTCTC20.19723865877712032No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA20.19723865877712032No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT20.19723865877712032No Hit
CAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGA20.19723865877712032No Hit
GTATGGAGTTCTCACTCACTGACCCGAGACTGGAGCCACACAAATGGGAA20.19723865877712032No Hit
CACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATA20.19723865877712032No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC20.19723865877712032No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA20.19723865877712032No Hit
GTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGC20.19723865877712032No Hit
TCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGA20.19723865877712032No Hit
CTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAA20.19723865877712032No Hit
GATAAGTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATT20.19723865877712032No Hit
CTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCCTCT20.19723865877712032No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA20.19723865877712032No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20.19723865877712032No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT20.19723865877712032No Hit
GGCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAA20.19723865877712032No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT20.19723865877712032No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC20.19723865877712032No Hit
CTTTTACTCTCGAACATGTACCCTTTCCCTAGTCTTGCCATTTTGTTTGA20.19723865877712032No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG20.19723865877712032No Hit
GCATTGACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAA20.19723865877712032No Hit
TCCTTTTATAATGAACCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGG20.19723865877712032No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)