FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTGGGACCAAAACCTCTAGTACAGATTGCATTAAACTTACAGTTTTT14.545454545454546No Hit
GCCCTGTACTAGTTTTGCTTCTTTGACCAAGAGCTGACATAAGAAGGTGA14.545454545454546No Hit
CATCTATTCGGTGGCATGAGGGTGGTCGTTGTAGCGGTGCTGGTGGTTGG14.545454545454546No Hit
GGGGGGGGGGGGGGGGGTGGGGGGAGGGGGGTGGGGGGGCGGGGGGGGGG14.545454545454546No Hit
GGTCTATTACAGGTACCCTCCCTTTCGGTTCTCACCAAAACCAGGATGAT14.545454545454546No Hit
CATCCACACAGCTTCCGATCACAGCGGGCTCTCCCTGCTGGTACCTACAG14.545454545454546No Hit
CATAAGAGCTGAGTGTTTCCGGTAAGGTCATACTCACAGGAATGTTACCA14.545454545454546No Hit
ATATTCTATTATAGTTGTATTTCTGTGGCGTTTTAATTTATCCTGTTTCA14.545454545454546No Hit
CTGTTCAAGGTGCTATTAGCCTGTATTGTCCCTGTGCCTCTGACGGTGTC14.545454545454546No Hit
TATTTGGACAGTGTCAAATGTCCCAAGAAAATCCCGCATTTGCTGGAAAA14.545454545454546No Hit
TAATTCTCCAGGCTCTCTTCCCTCATCTGGGAGAGGATCCAGCAAACCCA14.545454545454546No Hit
GAACTGGACCCAGAATGGAAATAGAAGGGCTCTTAAGAAAAAAGCATGGA14.545454545454546No Hit
TCTCTCCCCTGGGGAAGGTTCATTAATTGTCGGTAGCTTGTCTCTCTCCA14.545454545454546No Hit
CCTTAAATCCACCAAAATACTGAGGTCACATGGCAAACTGCTGCTCTAAA14.545454545454546No Hit
CAATGGCAGATCCCCCATTCCATGATCCGCTCCCCCGACATCAAAAATCC14.545454545454546No Hit
CCTTGGGGGTTATGCTCACCGGCCCCCGACGGATTCGGTGACGGGACCTA14.545454545454546No Hit
GTATCGCCCTCTTAACAATATTGTTCTTACAAGCCAGTAGACTGGATTGG14.545454545454546No Hit
CACCGACGCCCATCTCAGCACCGCGCCAGTAACTCCCACATCAGCATCAG14.545454545454546No Hit
GCTACATACTCTCCTCATACTCAGATAATCGTCTTTATGGCCACACAGTG14.545454545454546No Hit
GTAGGGGAGAGCCTAAACGCCCGGAGACCAGCAGGTGCTATAACGCGAGA14.545454545454546No Hit
CCACAAAAAACTAGATAACGCTGAGAGGGAAAACAGTACGAAAAAAGGGA14.545454545454546No Hit
TGTCATAATAAATCTATTTAAAGTACATTTTCAGTATTATGTAAATATCA14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)