FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_G11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_G11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG130.41881443298969073No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC110.3543814432989691No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA100.3221649484536082No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC80.25773195876288657No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT70.22551546391752575No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT70.22551546391752575No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT70.22551546391752575No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG70.22551546391752575No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT60.19329896907216496No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA60.19329896907216496No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT60.19329896907216496No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC60.19329896907216496No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC60.19329896907216496No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA60.19329896907216496No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAG60.19329896907216496No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA60.19329896907216496No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG60.19329896907216496No Hit
GGATGCTCTGAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGGGAA60.19329896907216496No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC60.19329896907216496No Hit
GAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTATCTAATA60.19329896907216496No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT50.1610824742268041No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA50.1610824742268041No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC50.1610824742268041No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG50.1610824742268041No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG50.1610824742268041No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT50.1610824742268041No Hit
GATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGAT50.1610824742268041No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA50.1610824742268041No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG50.1610824742268041No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC50.1610824742268041No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.1610824742268041No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA50.1610824742268041No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT50.1610824742268041No Hit
CTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGATTTA50.1610824742268041No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT50.1610824742268041No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG40.12886597938144329No Hit
ATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTC40.12886597938144329No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA40.12886597938144329No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC40.12886597938144329No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG40.12886597938144329No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT40.12886597938144329No Hit
CTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGA40.12886597938144329No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA40.12886597938144329No Hit
GAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG40.12886597938144329No Hit
GAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCATCTTGATCTTGGAG40.12886597938144329No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG40.12886597938144329No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC40.12886597938144329No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA40.12886597938144329No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT40.12886597938144329No Hit
GAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGG40.12886597938144329No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG40.12886597938144329No Hit
GTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTA40.12886597938144329No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA40.12886597938144329No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA40.12886597938144329No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA40.12886597938144329No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT40.12886597938144329No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG40.12886597938144329No Hit
TTCTTTGGCTGGAAAGAGCCTAACATAGTCAAACCACATGAGAAAGGCAT40.12886597938144329No Hit
GAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCA40.12886597938144329No Hit
GCTTGAATCAGTCAATTCACATGCCTTATTGAATTCATTTTGGACCCAGC40.12886597938144329No Hit
CGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTAT40.12886597938144329No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG40.12886597938144329No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC40.12886597938144329No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG40.12886597938144329No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT40.12886597938144329No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCA40.12886597938144329No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)