FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n02_FD_S3_R2_G05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n02_FD_S3_R2_G05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAATAGACAAAATGGCAGAACCACTTCTAGCAATGAAAACAGTTCTCA13.125No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCACGAGTGGTCAG13.125No Hit
TGATCATTCTGATTAACTACATTGCTATTGATCAAACTCCTATCACCGCA13.125No Hit
GACCCTCTCTCGGCAGCGTGCCGGCCGGGCCCGCCGCCCCTCCGGCCCTG13.125No Hit
ATTCGACACTAAATGATGGCCATCCGAAGTCTATTGGACGCTGTCTGGCT13.125No Hit
TTCAATAATACAAGCGGCACATAAGTCAAAAAAGAACATTAACTAATTAC13.125No Hit
CAACATGACCAAGAAGATGGTCACGCATAGAACAATAGGGAAGAAAAAAC13.125No Hit
CATCCACAGCACTATTCTCTTCCTGTTGATATTCTTCCATCATGGACTAA13.125No Hit
AAATTGCATCCTACTCTTTTCTGGTGTGTAAGCATCAAAGGGGAGAATTG13.125No Hit
GCTTTGGCAAGAGATAATGGTGGCTTGAACTAGTATGGGAGTCATAAAGT13.125No Hit
AGCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAGAACCGGTTCATT13.125No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG13.125No Hit
ACTGAGCAACGCCTGCTTAAGGGCTCACTACCACACCCCTGAGCGCATGA13.125No Hit
AGGGTGGAACCCCTCAGGCCCGAGCGTGGCCCTGCACCCGCAGCACCGCT13.125No Hit
ATAAAAGGAAGGTCTCAGTTGAGAAATGAGACTGATGTGGTGAACTTTGT13.125No Hit
GGCTCAGGCCCTCGGGGGGTTGGGGGGGGTCTGTTGGGGGGGTTGTGTGG13.125No Hit
CTAATGGGAATAACTGAGCTAGCATAATGTCCAACAGGTTCTTAAACAGA13.125No Hit
TTTCCAAGACAGATCCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACT13.125No Hit
CTTGCGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGTGA13.125No Hit
GAGCAAGTACTCAATAAATATTTATTTGTTCAGCATAGTGAGATATGAAA13.125No Hit
CTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGC13.125No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA13.125No Hit
GAAGACAGCAGCGCAAGAATAAAAGCTACGCTTTTCACTATAAGAAAAGA13.125No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA13.125No Hit
AAATATCTACAGAATAAAAGAAATGAGAGATCTAATCTCGCAGTCCCGCA13.125No Hit
GATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTG13.125No Hit
GCCGGGGCGCACGGCAGTCTGCGTGCCGCCGGGCACCAACAAAGTGATGA13.125No Hit
GATTGAGAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATGA13.125No Hit
CTCCGGAGGTAGTTGCAATGGCTGGGATAATCTGGCCCAAATTTAGCTTG13.125No Hit
GTGTGACATTGGGCCAGAGCCTTGGGAAGGGTCACGTAGGGCGGCAGATA13.125No Hit
CGTATAATGTATAAGTTTGGTCCTCCATCTCATACTAATAGCCCTACCTT13.125No Hit
ACACAAGAGCATATCTCCTATTTCCAGAACACAGTAATTTTCACATTAGT13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)